Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

SPIM

SPIM 3D reconstruction

Single plane illumination microscopy (SPIM) in an upright configuration allows for fast 3D imaging of single cells grown on glass coverslips, and of large, multicellular organisms such as zebrafish embryos.

The custom-built SPIM system at the LFD can simultaneously image two color channels, e.g. the nucleus (blue, H2B-EGFP) and mitochondria (green, TMRE) of live CHO-K1 cells (shown).


News

Welcome to the LFD

The Laboratory for Fluorescence Dynamics (LFD) is a national research resource center for biomedical fluorescence spectroscopy, supported by the National Institute of General Medical Sciences (NIGMS, 8P41GM103540) divisions of the National Institutes of Health (NIH) and the University of California, Irvine (UCI).

The founder and Principal Investigator of the LFD is Enrico Gratton. The Co-Investigator is Michelle Digman.

The LFD's main activities are:

  • Services and Resources: the LFD provides a state-of-the-art laboratory for fluorescence measurements, microscopy and spectroscopy, with technical assistance to visiting scientists.
  • Research and Development: the LFD designs, tests, and implements advances in the technology of hardware, software, and biomedical applications.
  • Training and Dissemination: the LFD disseminates knowledge of fluorescence spectroscopic principles, instrumentation, and applications to the scientific community.

Read more about the LFD's activities, history, funding, and organization.