Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Notes

Refer to the visitors guide for location and directions.

Confirm date and location with the LFD Front Desk.

LFD Colloquium

Every week or two, invited guests or members of the Laboratory for Fluorescence Dynamics (LFD) present their work or review related literature. The audience is expected to ask questions and to evaluate the work presented.

The events are free and open to the public.

Location: Natural Sciences II, Room 3201, University of California, Irvine.

Upcoming

Past

  • Thursday, April 20, 2017. 15:00

    Enrico Gratton: Tracking with the STED microscope: when resolution matters.

  • Thursday, February 2, 2017. 15:00

    Enrico Gratton: TTBA

2016

  • Friday, December 2, 2016. 13:00

    Rachel Cinco: Redox state and ovarian function

  • Thursday, November 17, 2016. 15:30

    Estella Rao: Epidermal Growth Factor Receptor (EGFR) diffusion across the membrane.

  • Thursday, November 10, 2016. 15:30

    Ylenia Santoro: Improving diagnosis of breast cancer using a noninvasive optical technique.

  • Thursday, October 20, 2016. 14:30

    Enrico Gratton: Measuring molecular diffusion in live cells using camera.

  • Tuesday, October 11, 2016. 15:30

    Suman Ranjit: Spatial dynamics of SIRT1 dictate metabolic transitions in the cell nucleus.

  • Thursday, September 22, 2016. 15:30

    Per Niklas Hedde: SPIM in the conventional sample geometry.

  • Wednesday, September 14, 2016. 15:30

    Enrico Gratton: New methods for 2-dimensional fluctuation analysis (continued).

  • Wednesday, September 7, 2016. 15:30

    Enrico Gratton: New methods for 2-dimensional fluctuation analysis (continued).

  • Wednesday, August 31, 2016. 16:00

    Enrico Gratton: New methods for 2-dimensional fluctuation analysis.

  • Wednesday, May 4, 2016. 15:00

    Enrico Gratton: Measurements of absolute concentration of NADH in cells using the phasor FLIM method.

2015

  • Tuesday, December 1, 2015. 11:00

    Enrico Gratton: Multi-dimensional phasor analysis.

  • Tuesday, September 22, 2015. 10:00

    Enrico Gratton: 2D pair correlation functions diffusion tensor connectivity maps.

  • Thursday, July 30, 2015. 11:00

    Enrico Gratton: TTBA.

  • Tuesday, June 16, 2015. 11:00

    Elina Staaf (Karolinska Institutet, Sweden): TTBA.

  • Wednesday, June 10, 2015. 15:00

    Rupsa Datta: Fluorescence lifetime imaging of endogenous biomarker of oxidative stress.

  • Wednesday, March 4, 2015. 15:00

    Carmine di Rienzo: TTBA.

    Per Niklas Hedde: TTBA.

  • Thursday, February 26, 2015. 14:00

    Belinda Wright (University of Western Sydney, School of Science and Health): Analysis of NADH autofluorescence in early differentiated rat myoblasts and developing Zebrafish skeletal mussle tissue.

2014

  • Wednesday, December 3, 2014. 14:00

    Christopher Heylman: Non-invasive image-based assessment of human induced pluripotent stem cell-derived cardiomyocytes.

  • Monday, November 24, 2014. 15:30

    Christian Poulsen (University of Copenhagen, Denmark): TTBA.

    Nadir Planes (INDICASAT-AIP, Panama): TTBA.

  • Wednesday, October 8, 2014. 12:00

    Enrico Gratton: The Nobel Prize in Chemistry 2014 was awarded jointly to Eric Betzig, Stefan W. Hell and William E. Moerner "for the development of super-resolved fluorescence microscopy".

  • Thursday, September 18, 2014. 14:00

    Suman Ranjit: Three photon excitation FCS.

  • Thursday, August 21, 2014. 10:00

    Enrico Gratton: iMSD.

  • Friday, August 1, 2014. 10:00

    Julian Pohlan: TTBA.

  • Tuesday, July 8, 2014. 11:00

    Christopher Heylman: Noninvasive image based assessment of engineered human cardiac tissues.

  • Thursday, May 29, 2014. 15:00

    Agustina Olivera-Couto (Institut Pasteur de Montevideo, Uruguay): Protein dynamics in eisosomal plasma membrane domains.

  • Thursday, May 22, 2014. 10:30

    Himanshu Sharma (Chemical Engineering and Materials Science, University of California, Irvine): Shrink-induced wrinkled structures for enhanced fluorescence sensing.

  • Thursday, May 15, 2014. 9:30

    Manuela Gabriel (Physics Department, University of Buenos Aires, Argentina): Characterization of metallic nanoparticles as alternative probes to fluorescence microscopy and structural studies at the nanoscale.

  • Tuesday, April 15, 2014. 14:30

    Elina Staaf (Karolinska Institutet, Sweden): Natural killer cells: assessing dynamics and interactions at single molecule level.

  • Tuesday, February 25, 2014. 15:00

    Dolores Carrer (Ferreyra Research Institute, Córdoba, Argentina): Skin architecture and liposomes epidermal penetration: two-photon microscopy insights.

  • Thursday, February 20, 2014. 15:00

    Ylenia Santoro: Design of a rectal probe for diffuse optical spectroscopy imaging for chemotherapy and radiotherapy monitoring.

  • Thursday, February 6, 2014. 15:30

    Enrico Gratton: Image Mean Square Displacement (iMSD)

  • Thursday, January 30, 2014. 14:00

    Alexander Small (California State Polytechnic University, Pomona): Theory of super-resolution.

  • Thursday, January 9, 2014. 16:00

    Enrico Gratton: New developments in tICS.

2013

  • Tuesday, November 5, 2013. 16:00

    Geoffrey Harlow (Department of Botany & Plant Sciences, University of California, Riverside): Quantifying cell functional traits.

  • Wednesday, August 28, 2013. 15:00

    Rupsa Datta: Study of indices of metabolic activity at single cell level by optical imaging.

  • Tuesday, August 27, 2013. 15:30

    Enrico Gratton: Single point FCS in whole images: is it feasible?

  • Thursday, July 18, 2013. 11:00

    Parkson Lee-Gau Chong (Department of Biochemistry, Temple University): Physical properties of archaeal tetraether liposomes.

  • Wednesday, May 1, 2013. 15:30

    Belinda Wright (University of Western Sydney, Australia): Real-time analysis of endogenous NADH autofluorescence in live progenitor stem cells.

  • Tuesday, March 12, 2013. 13:30

    Paolo Annibale: Investigating the impact of single molecule fluorescence dynamics on photoactivated localization microscopy experiments.

  • Friday, March 1, 2013. 11:00

    Agustina Olivera-Couto (Institut Pasteur de Montevideo, Uruguay): In vivo fluorescence microscopy analysis of membrane domains called Eisosomes in Yeast.

  • Friday, February 15, 2013. 11:30

    Matthew J Paszek (University of California, San Francisco): Imaging cellular adhesion at the nanoscale reveals that the glycocalyx mechanically activates integrin function.

2012

  • Monday, October 22, 2012. 17:30

    Scott Jordan (Physik Instrumente): Recent advancements in high-throughput interfacing of relevance to fluorescence microscopy.

  • Monday, September 10, 2012. 14:30

    Nicoletta Plotegher: Alpha-synuclein aggregation process and related toxic mechanisms in live cells.

  • Friday, July 13, 2012. 14:00

    Shanshan Xu: Characterization of breast cancer using an endogenous tumor spectral marker: high-spectral-resolution diffuse optical spectroscopy and its clinical applications.

  • Thursday, May 3, 2012. 15:00

    Ottavia Golfetto: Laurdan fluorescence lifetime as a biosensor of changes in membrane of living cells.

  • Monday, April 2, 2012. 12:00

    Maxime Dahan (Ecole normale supérieure, Paris; and Janelia Farm Research Center): Investigating the target search of DNA-binding proteins in mammalian cells at the single molecule level.

  • Thursday, March 8, 2012. 16:30

    Chiara Stringari: Review of YP Hung et al. Imaging cytosolic NADH-NAD(+) redox state with a genetically encoded fluorescent biosensor. Cell Metab. 2011;14(4):545-54. PMCID: PMC3190165.

  • Thursday, February 23, 2012. 16:00

    Shanshan Xu: Characterization of breast cancer using an endogenous malignancy specific tumor biomarker.

  • Wednesday, February 8, 2012. 16:00

    Chi-Li Chiu: Measuring actin flow in cell protrusions in 3D.

  • Thursday, February 2, 2012. 16:30

    Maria Teresa Fernandez Luna (Department of Entomology, University of California, Riverside): Aggregation state of Cyt1A toxin in the cytoplasm membrane of Culex quinquefasciatus mosquito midgut cells.

  • Tuesday, January 31, 2012. 16:30

    Viera Crosignani: Deep in vivo enhanced resolution imaging system.

  • Wednesday, January 25, 2012. 16:00

    Ylenia Santoro and Cosimo Arnesano: Digital frequency-domain parallel spectroscopy for tissue imaging.

2011

  • Thursday, August 18, 2011. 15:00

    Emmanuel Adu-Gyamfi (University of Notre Dame, South Bend, IN): Targeting Ebola virus at the plasma membrane.

  • Friday, August 12, 2011. 9:00

    Undergraduate Student Initiative for Biomedical Research (USIBR)

  • Thursday, August 4, 2011. 15:00

    Enrico Gratton: Data processing in the phasor analysis of FLIM.

  • Thursday, July 28, 2011. 15:00

    Mark Jones and Laura Andrews (University of Western Sydney, Richmond, Australia): Understanding the intracellular dynamics of RNA.

  • Wednesday, July 13, 2011. 10:00

    Ylenia Santoro: Heterogeneity in breast lesions.

  • Wednesday, July 6, 2011. 10:00

    Enrico Gratton: STICS - statistical analysis of trajectories.

  • Wednesday, June 29, 2011. 10:00

    Viera Crosignani: In vivo deep tissue imaging.

  • Thursday, May 19, 2011. 15:30

    Stefano Coppola and Federica Sabatini (Facoltà di SMFN, Sapienza University of Rome, Italy): Single particle tracking of lipoplexes in live cells.

  • Wednesday, May 11, 2011. 10:00

    Enrico Gratton: The FLIM camera project.

  • Thursday, March 31, 2011. 14:00

    Laura Estrada: A 3D method to map fibers inside cells using nanoparticles as optical antennas.

  • Wednesday, March 23, 2011. 10:00

    Luca Lanzanò: Measurement of distance with the Modulation Tracking method.

  • Thursday, March 17, 2011. 14:00

    Chi-Li Chiu: Cell-matrix adhesion function on cell protrusion in 3D shown by modulation tracking.

  • Wednesday, February 23, 2011. 10:00

    Elizabeth Hinde: Biosensor FRET detection: a comparative study of the phasor approach to FLIM with ratiometric analysis in live cells.

  • Wednesday, February 16, 2011. 10:00

    Chiara Stringari: In vivo identification of changes in metabolic state as stem cells differentiate, by phasor analysis of fluorescence lifetime imaging.

  • Wednesday, February 9, 2011. 9:30

    Francesco Cutrale: Oblique Light Sheet Microscopy.

2010

  • Friday, December 3, 2010. 10:00

    Shanshan Xu: Characterization of breast lesion using endogenous tumor spectral marker: high-spectral-resolution diffuse optical spectroscopy.

  • Friday, October 29, 2010. 16:00

    Christopher Hammang (School of Natural Sciences, University of Western Sydney, Australia): Actin in the nucleus.

  • Thursday, September 9, 2010. 10:00

    Elizabeth Hinde: Pair correlation analysis of intranuclear diffusion.

  • Tuesday, August 31, 2010. 10:00

    Cosimo Arnesano: Deep tissue raman spectroscopy in vivo.

  • Friday, August 27, 2010. 10:00

    Peter Fwu: Nanometer-scale imaging technique: modulation tracking.

  • Thursday, August 12, 2010. 10:00

    Ylenia Santoro: Characterization of breast cancer and prediction of chemotherapy response via near-infrared biomarkers.

  • Wednesday, July 28, 2010. 15:00

    Professor Ulrike Alexiev (Department of Physics, Universität Berlin, Germany): Dynamics and function of protein nanomachines: from membranes to single molecules.

  • Tuesday, May 11, 2010. 14:00

    Giulia Ossato: Protein aggregation in neurodegenerdative disease.

  • Thursday, April 1, 2010. 10:00

    Kaveh Azartash: A technique to quantify the precorneal tear film and its components.

  • Thursday, March 11, 2010. 10:00

    Laura Estrada: Near field fluorescence correlation spectroscopy using metallic nanoparticles.

  • Thursday, March 4, 2010. 14:00

    Alexander Dvornikov: Imaging in depth in scattering media by two-photon fluorescence microscopy.

  • Wednesday, February 3, 2010. 10:00

    Chiara Stringari: Multiple components mapping of live tissue by phasor analysis of fluorescence lifetime imaging.

  • Wednesday, January 27, 2010. 10:00

    Thomas Gensch (Institute of Structural Biology and Biophysics 1, Forschungszentrum Jülich, Germany): Fluorescence microscopy & spectroscopy: from protein conformational changes to intracellular ion concentrations & superresolution.

  • Thursday, January 14, 2010. 10:00

    Enrico Gratton: Imaging nanostructures in live cells.

2009

  • Thursday, December 3, 2009. 14:00

    Francesco Cardarelli: In vivo imaging of single molecule translocation through nuclear pore complexes by pair correlation function.

  • Tuesday, November 17, 2009. 10:00

    Bo Chen: Nanometer-scale imaging of collagen fibers using gold beads.

  • Monday, October 5, 2009. 10:00

    Michelle Digman: Raster image correlation spectroscopy (RICS).

    Michelle Digman: Number and brightness (N & B) analysis.

  • Thursday, October 1, 2009. 9:30

    Giulia Ossato: Huntingtin (Htt) oligomer formation in live cells.

    Luca Lanzanò: Particle tracking.

  • Thursday, September 3, 2009. 10:00

    Chiara Stringari: Phasor analysis of fluorescence lifetime imaging microscopy.

  • Thursday, August 20, 2009. 10:00

    Hongtao Chen: Advanced instrumentation for fluorescence imaging.

  • Thursday, August 13, 2009. 10:00

    Peter Fwu: nrCT application on the 3D microvillus imaging.

  • Wednesday, July 22, 2009. 10:00

    Enrico Gratton: New features of SimFCS (part 2).

  • Friday, July 17, 2009. 12:00

    Enrico Gratton: New features of SimFCS.

  • Wednesday, July 8, 2009. 10:00

    Enrico Gratton: The structure and protein distribution in microvilli of renal cells by nrCT.

  • Wednesday, July 1, 2009. 10:00

    Bo Chen: Labeling collagen fibers with gold beads.

  • Wednesday, June 24, 2009. 10:00

    William Mantulin: Review of TW Gadella and TM Jovin. Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy: A stereochemical model for tyrosine kinase receptor activation. J Cell Biol. 1995. 129(6): 1543-1558. PMCID: PMC2291181.

  • Wednesday, June 17, 2009. 10:00

    Enrico Gratton: Imaging barriers to diffusion by pair correlation functions.

  • Wednesday, June 10, 2009. 14:30

    Hongtao Chen (Chemistry, Purdue University): In vitro and in vivo studies of drug delivery systems by fluorescence and non-linear optical methods.

  • Thursday, June 4, 2009. 14:00

    Enrico Gratton: More selected topics in tissue engineering.

  • Friday, May 29, 2009. 14:30

    Enrico Gratton: Selected topics in tissue engineering.

  • Thursday, May 21, 2009. 14:00

    Enrico Gratton and Kaveh Azartash: Recent developments in fluctuation analysis of speckle technique for tearfilm studies.

  • Thursday, May 14, 2009. 14:00

    Molly Rossow: Analysis of flow using spatial-temporal correlation.

  • Friday, April 3, 2009. 11:30

    Shanshan Xu: Intrinsic near-infrared spectroscopic markers of breast tumors: a review of S. Kukreti's publication and future directions for the project.

  • Wednesday, March 18, 2009. 10:00

    William Mantulin: Review of MA Digman et al. Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method. Biophys J. 2009; 96(2): 707-716. doi:10.1016/j.bpj.2008.09.051.

  • Wednesday, March 11, 2009. 15:00

    Enrico Gratton and Luca Lanzanò: Coherent motion of cell layers.

  • Wednesday, February 25, 2009. 10:00

    Enrico Gratton: Diffusion or binding? The PSF is the answer.

  • Wednesday, February 18, 2009. 10:00

    Kaveh Azartash: A new technique for measuring the dynamics of tearfilm.

  • Wednesday, February 11, 2009. 14:30

    Molly Rossow: Applications of scanning laser image correlation (SLIC).

  • Tuesday, February 3, 2009. 11:30

    Milka Stakic and Jennifer Sasaki: Review of AD Elder et al. A quantitative protocol for dynamic measurements of protein interactions by Förster resonance energy transfer-sensitized fluorescence emission. J R Soc Interface (2009) 6, S59–S81. doi:10.1098/rsif.2008.0381.focus.

  • Wednesday, January 28, 2009. 10:00

    Molly Rossow: Scanning laser image correlation (SLIC).

  • Wednesday, January 21, 2009. 10:00

    Luca Lanzanò: Delayed luminescence as a tool to Investigate the biological organization.

  • Wednesday, January 14, 2009. 10:00

    Francesco Cardarelli (Molecular Biophysics, Scuola Normale Superiore, Pisa, Italy): Nanostructuring HIV-1 Tat arginine-rich motif for targeted cellular delivery.

  • Wednesday, January 7, 2009. 13:30

    Enrico Gratton: Comparing light sources for fluorescence microscopy and spectroscopy applications.

    Carole Lezers (Lumencor, Inc., Beaverton, OR): a demonstration of the Lumencor light engine.

2008

  • Wednesday, December 17, 2008. 11:00

    Michelle Digman: Imaging breast cancer cells in 3D matrices.

  • Tuesday, December 2, 2008. 11:00

    Enrico Gratton: The pair correlation function.

  • Wednesday, November 26, 2008. 10:00

    Enrico Gratton: The new FlimBox: control of excitation and emission from picoseconds to seconds.

  • Wednesday, November 12, 2008. 9:30

    Christian Hellriegel, Valeria Caiolfa and Moreno Zamai (Microscopy and Imaging Unit, Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain): uPAR-uPA binding stoichiometry in live cells.

  • Wednesday, November 5, 2008. 10:00

    Enrico Gratton: What is ratiometric FRET imaging and how to do it.

  • Wednesday, October 22, 2008. 10:00

    Michelle Digman: Number & brightness analysis.

  • Wednesday, October 15, 2008. 10:00

    Giulia Ossato: Applications of N&B: Huntingtin (Htt) oligomer formation in live cells.

  • Thursday, October 2, 2008. 13:00

    Enrico Gratton and Michelle Digman: Novel methods to calculate protein interaction in live cells.

  • Wednesday, September 24, 2008. 15:30

    Kaveh Azartash: Measuring the cell induced deformation of collagen with digital holographic microscopy.

  • Wednesday, September 17, 2008. 15:30

    Valeria Vetri: Concanavalin A aggregation processes: insight on amyloid cytotoxicity.

  • Wednesday, September 10, 2008. 15:30

    Giulia Ossato: N & B fluctuation analysis in studying Huntingtin protein aggregation in live cells.

  • Wednesday, August 27, 2008. 13:00

    Francesco Cutrale: PALM: photoactivated localization microscopy.

  • Wednesday, August 13, 2008. 15:00

    Enrico Gratton: Pair correlation functions.

  • Wednesday, August 6, 2008. 15:00

    Peter Fwu: Particle tracking: research directions.

  • Wednesday, July 30, 2008. 13:00

    Enrico Gratton: The co-variance principle and the derivation of the cross-correlation number and brightness method.

  • Wednesday, July 23, 2008. 14:30

    Enrico Gratton: Cross-variance and binding dynamics.

  • Friday, June 20, 2008. 15:30

    Ryan Colyer: Development of a fluorescence lifetime based method to detect and analyze single molecule reactions in solution.

  • Friday, May 9, 2008. 15:30

    Enrico Gratton: Discussion of recent developments at the LFD: fluorescence lifetime imaging (FLIM), Globals for Images software development, and collaborative applications of LFD techniques and technologies.

  • Tuesday, May 6, 2008. 14:30

    Christian Hellriegel: 3D single particle tracking with gold nanoparticles in collagen.

  • Thursday, May 1, 2008. 14:30

    Ryan Colyer: Direct observation of conformational dynamics in Calmodulin with a phasor trajectory analysis.

    Christoph Gohlke: Training and dissemination at the LFD.

  • Tuesday, April 29, 2008. 14:30

    Kandice Tanner: New developments: cell migration in collagen matrices.

    Susana Sanchez: Interaction of rHDL particles with cells.

  • Tuesday, April 15, 2008. 9:30

    Michelle Digman: Cross number and brightness (N&B) correlation.

  • Tuesday, April 8, 2008. 10:30

    Enrico Gratton: Cross number & brightness (N&B): new applications for 2-color aggregation analysis.

  • Thursday, March 20, 2008. 10:30

    Sergio Perfetto: Development of an instrument for measuring extremely low concentrations of particles.

    Enrico D'Amico: Digital frequency-domain fluorescence lifetime imaging system: new features and directions.

  • Monday, March 3, 2008. 13:00

    Ryan Colyer: Development of a fluorescence lifetime based method to analyze single molecule reactions and applications to the conformation dynamics of calmodulin.

  • Wednesday, February 27, 2008. 13:30

    Kandice Tanner: Cell migration in collagen matrices. Assessment of local stiffness by fluctuation spectroscopy.

  • Wednesday, February 20, 2008. 13:30

    Enrico D'Amico and Sergio Perfetto: The return of the FlimBox.

  • Thursday, February 14, 2008. 14:15

    Theodore L Hazlett: Membrane diffusion measured using fluorescence correlation spectroscopy in polarized S. cerevisiae.

  • Friday, February 1, 2008. 12:00

    Claus Seidel (Molekulare Physikalische Chemie, Heinrich Heine Universität Düsseldorf, Germany): Biomolecular function studied by multiparameter fluorescence imaging and spectroscopy.

  • Thursday, January 24, 2008. 12:00

    Jay Unruh: Total internal reflection microscopy (TIRF) instrumentation.

  • Wednesday, January 16, 2008. 13:00

    Molly Rossow: Blood flow mapping through spatial temporal image correlation.

2007

  • Friday, December 14, 2007. 10:00

    Christian Hellriegel: Collagen and Au nanoparticles.

  • Friday, December 7, 2007. 10:00

    Molly Rossow: Near infrared spatial temporal image correlation spectroscopy.

  • Thursday, November 15, 2007. 14:00

    Jay Unruh: N&B analysis of uPAR aggregation on the basal membrane of living cells.

  • Thursday, November 8, 2007. 13:30

    Ryan Colyer: Development of a fluorescence lifetime based method to detect and analyze single molecule reactions in solution.

  • Thursday, October 18, 2007. 11:30

    Michelle Digman: Applications of RICS.

  • Monday, September 24, 2007. 15:30

    Paolo Minzioni (Postdoctoral scholar, Electronics Department, University of Pavia, Italy): A new approach to single-fiber optical tweezers: an integrated probe for all-optical 3D-trapping, manipulation and analysis.

  • Friday, April 20, 2007. 15:00

    Yun Chen (Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill): Nanoscale signal transduction across the plasma membrane: how clustered GPI-anchored proteins accomplish transmembrane signalling.