LFD Colloquium

Every week or two, invited guests or members of the Laboratory for Fluorescence Dynamics (LFD) present their work or review related literature. The audience is expected to ask questions and to evaluate the work presented.

The events are free and open to the public.

Location: Room 3201, Natural Sciences 2 building, University of California, Irvine.

Upcoming

Past

• Tuesday, November 17, 2009. 10:00 AM

  Bo Chen: Nanometer-scale imaging of collagen fibers using gold beads.

• Monday, October 5, 2009. 10:00 AM

  Michelle Digman: Raster image correlation spectroscopy (RICS).

  Michelle Digman: Number and brightness (N & B) analysis.

• Thursday, October 1, 2009. 9:30 AM

  Giulia Ossato: Huntingtin (Htt) oligomer formation in live cells.

  Luca Lanzanò: Particle tracking.

• Thursday, September 3, 2009. 10:00 AM

  Chiara Stringari: Phasor analysis of fluorescence lifetime imaging microscopy.

• Thursday, August 20, 2009. 10:00 AM

  Hongtao Chen: Advanced instrumentation for fluorescence imaging.

• Thursday, August 13, 2009. 10:00 AM

  Peter Fwu: nrCT application on the 3D microvillus imaging.

• Wednesday, July 22, 2009. 10:00 AM

  Enrico Gratton: New features of SimFCS (part 2).

• Friday, July 17, 2009. 12:00 PM

  Enrico Gratton: New features of SimFCS.

• Wednesday, July 8, 2009. 10:00 AM

  Enrico Gratton: The structure and protein distribution in microvilli of renal cells by nrCT.

• Wednesday, July 1, 2009. 10:00 AM

  Bo Chen: Labeling collagen fibers with gold beads.

• Wednesday, June 24, 2009. 10:00 AM

  William Mantulin: Review of TW Gadella and TM Jovin. "Oligomerization of epidermal growth factor receptors on A431 cells studied by time-resolved fluorescence imaging microscopy: A stereochemical model for tyrosine kinase receptor activation." J Cell Biol. 1995. 129(6): 1543-1558. PMCID: PMC2291181.

• Wednesday, June 17, 2009. 10:00 AM

  Enrico Gratton: Imaging barriers to diffusion by pair correlation functions.

• Wednesday, June 10, 2009. 2:30 PM

  Hongtao Chen (Chemistry, Purdue University): In vitro and in vivo studies of drug delivery systems by fluorescence and non-linear optical methods.

• Thursday, June 4, 2009. 2:00 PM

  Enrico Gratton: More selected topics in tissue engineering.

• Friday, May 29, 2009. 2:30 PM

  Enrico Gratton: Selected topics in tissue engineering.

• Thursday, May 21, 2009. 2:00 PM

  Enrico Gratton and Kaveh Azartash: Recent developments in fluctuation analysis of speckle technique for tearfilm studies.

• Thursday, May 14, 2009. 2:00 PM

  Molly Rossow: Analysis of flow using spatial-temporal correlation.

• Friday, April 3, 2009. 11:30 AM

  Shanshan Xu: Intrinsic near-infrared spectroscopic markers of breast tumors: a review of S. Kukreti's publication and future directions for the project.

• Wednesday, March 18, 2009. 10:00 AM

  William Mantulin: Review of M. A. Digman et al. "Detecting protein complexes in living cells from laser scanning confocal image sequences by the cross correlation raster image spectroscopy method." Biophys J. 2009; 96(2): 707-716. doi:10.1016/j.bpj.2008.09.051.

• Wednesday, March 11, 2009. 3:00 PM

  Enrico Gratton and Luca Lanzanò: Coherent motion of cell layers.

• Wednesday, February 25, 2009. 10:00 AM

  Enrico Gratton: Diffusion or binding? The PSF is the answer.

• Wednesday, February 18, 2009. 10:00 AM

  Kaveh Azartash: A new technique for measuring the dynamics of tearfilm.

• Wednesday, February 11, 2009. 2:30 PM

  Molly Rossow: Applications of scanning laser image correlation (SLIC).

• Tuesday, February 3, 2009. 11:30 AM

  Milka Stakic and Jennifer Sasaki: Review of A. D. Elder et al. "A quantitative protocol for dynamic measurements of protein interactions by Förster resonance energy transfer-sensitized fluorescence emission." J R Soc Interface (2009) 6, S59–S81. doi:10.1098/rsif.2008.0381.focus.

• Wednesday, January 28, 2009. 10:00 AM

  Molly Rossow: Scanning laser image correlation (SLIC).

• Wednesday, January 21, 2009. 10:00 AM

  Luca Lanzanò: Delayed luminescence as a tool to Investigate the biological organization.

• Wednesday, January 14, 2009. 10:00 AM

  Francesco Cardarelli (Molecular Biophysics, Scuola Normale Superiore, Pisa, Italy): Nanostructuring HIV-1 Tat arginine-rich motif for targeted cellular delivery.

• Wednesday, January 7, 2009. 1:30 PM

  Enrico Gratton: Comparing light sources for fluorescence microscopy and spectroscopy applications.

  Carole Lezers (Lumencor, Inc., Beaverton, OR): a demonstration of the Lumencor light engine.

2008

• Wednesday, December 17, 2008. 11:00 AM

  Michelle Digman: Imaging breast cancer cells in 3D matrices.

• Tuesday, December 2, 2008. 11:00 AM

  Enrico Gratton: The pair correlation function.

• Wednesday, November 26, 2008. 10:00 AM

  Enrico Gratton: The new FlimBox: control of excitation and emission from picoseconds to seconds.

• Wednesday, November 12, 2008. 9:30 AM

  Christian Hellriegel, Valeria Caiolfa and Moreno Zamai (Microscopy and Imaging Unit, Fundación Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain): uPAR-uPA binding stoichiometry in live cells.

• Wednesday, November 5, 2008. 10:00 AM

  Enrico Gratton: What is ratiometric FRET imaging and how to do it.

• Wednesday, October 22, 2008. 10:00 AM

  Michelle Digman: Number & brightness analysis.

• Wednesday, October 15, 2008. 10:00 AM

  Giulia Ossato: Applications of N&B: Huntingtin (Htt) oligomer formation in live cells.

• Thursday, October 2, 2008. 1:00 PM

  Enrico Gratton and Michelle Digman: Novel methods to calculate protein interaction in live cells.

• Wednesday, September 24, 2008. 3:30 PM

  Kaveh Azartash: Measuring the cell induced deformation of collagen with digital holographic microscopy.

• Wednesday, September 17, 2008. 3:30 PM

  Valeria Vetri: Concanavalin A aggregation processes: insight on amyloid cytotoxicity.

• Wednesday, September 10, 2008. 3:30 PM

  Giulia Ossato: N & B fluctuation analysis in studying Huntingtin protein aggregation in live cells.

• Wednesday, August 27, 2008. 1:00 PM

  Francesco Cutrale: PALM: photoactivated localization microscopy.

• Wednesday, August 13, 2008. 3:00 PM

  Enrico Gratton: Pair correlation functions.

• Wednesday, August 6, 2008. 3:00 PM

  Peter Fwu: Particle tracking: research directions.

• Wednesday, July 30, 2008. 1:00 PM

  Enrico Gratton: The co-variance principle and the derivation of the cross-correlation number and brightness method.

• Wednesday, July 23, 2008. 2:30 PM

  Enrico Gratton: Cross-variance and binding dynamics.

• Friday, June 20, 2008. 3:30 PM

  Ryan Colyer: Development of a fluorescence lifetime based method to detect and analyze single molecule reactions in solution.

• Friday, May 9, 2008. 3:30 PM

  Enrico Gratton: Discussion of recent developments at the LFD: fluorescence lifetime imaging (FLIM), Globals for Images software development, and collaborative applications of LFD techniques and technologies.

• Tuesday, May 6, 2008. 2:30 PM

  Christian Hellriegel: 3D single particle tracking with gold nanoparticles in collagen.

• Thursday, May 1, 2008. 2:30 PM

  Ryan Colyer: Direct observation of conformational dynamics in Calmodulin with a phasor trajectory analysis.

  Christoph Gohlke: Training and dissemination at the LFD.

• Tuesday, April 29, 2008. 2:30 PM

  Kandice Tanner: New developments: cell migration in collagen matrices.

  Susana Sanchez: Interaction of rHDL particles with cells.

• Tuesday, April 15, 2008. 9:30 AM

  Michelle Digman: Cross number and brightness (N&B) correlation.

• Tuesday, April 8, 2008. 10:30 AM

  Enrico Gratton: Cross number & brightness (N&B): new applications for 2-color aggregation analysis.

• Thursday, March 20, 2008. 10:30 AM

  Sergio Perfetto: Development of an instrument for measuring extremely low concentrations of particles.

  Enrico D'Amico: Digital frequency-domain fluorescence lifetime imaging system: new features and directions.

• Monday, March 3, 2008. 1:00 PM

  Ryan Colyer: Development of a fluorescence lifetime based method to analyze single molecule reactions and applications to the conformation dynamics of calmodulin.

• Wednesday, February 27, 2008. 1:30 PM

  Kandice Tanner: Cell migration in collagen matrices. Assessment of local stiffness by fluctuation spectroscopy.

• Wednesday, February 20, 2008. 1:30 PM

  Enrico D'Amico and Sergio Perfetto: The return of the FlimBox.

• Thursday, February 14, 2008. 2:15 PM

  Theodore L Hazlett: Membrane diffusion measured using fluorescence correlation spectroscopy in polarized S. cerevisiae.

• Friday, February 1, 2008. 12:00 PM

  Claus Seidel (Molekulare Physikalische Chemie, Heinrich Heine Universität Düsseldorf, Germany): Biomolecular function studied by multiparameter fluorescence imaging and spectroscopy.

• Thursday, January 24, 2008. 12:00 PM

  Jay Unruh: Total internal reflection microscopy (TIRF) instrumentation.

• Wednesday, January 16, 2008. 1:00 PM

  Molly Rossow: Blood flow mapping through spatial temporal image correlation.

2007

• Friday, December 14, 2007. 10:00 AM

  Christian Hellriegel: Collagen and Au nanoparticles.

• Friday, December 7, 2007. 10:00 AM

  Molly Rossow: Near infrared spatial temporal image correlation spectroscopy.

• Thursday, November 15, 2007. 2:00 PM

  Jay Unruh: N&B analysis of uPAR aggregation on the basal membrane of living cells.

• Thursday, November 8, 2007. 1:30 PM

  Ryan Colyer: Development of a fluorescence lifetime based method to detect and analyze single molecule reactions in solution.

• Thursday, October 18, 2007. 11:30 AM

  Michelle Digman: Applications of RICS.

• Monday, September 24, 2007. 3:30 PM

  Paolo Minzioni (Postdoctoral scholar, Electronics Department, University of Pavia, Italy): A new approach to single-fiber optical tweezers: an integrated probe for all-optical 3D-trapping, manipulation and analysis.

• Friday, April 20, 2007. 3:00 PM

  Yun Chen (Department of Cell and Developmental Biology, University of North Carolina School of Medicine, Chapel Hill): Nanoscale signal transduction across the plasma membrane: how clustered GPI-anchored proteins accomplish transmembrane signalling.

• Friday, January 26, 2007. 2:30 PM–5:30 PM

  Trevor Smith (Ultrafast and Micro-Spectroscopy Laboratories School of Chemistry, University of Melbourne, Australia): Time-resolved total internal reflection fluorescence studies of macromolecular adsorption.

  Karsten Koenig (Institute of Anatomy II, Friedrich-Schiller University Jena, Germany): Time-resolved multiphoton tomography of human skin.

  Damian Bird (Ultrafast and Micro-Spectroscopy Laboratories School of Chemistry, University of Melbourne, Australia): Two-photon fluorescence lifetime microscopy of porphyrin photosensitizers for photodynamic therapy.