Features

• Developed by Enrico Gratton
• Fluorescence data analysis, simulation, and acquisition
• Fluorescence Correlation Spectroscopy (FCS)
• Fluorescence Lifetime Imaging (FLIM) and Phasor plots
• Förster Resonance Energy Transfer (FRET)
• Generalized Polarization (GP)
• Number and Brightness (N&B)
• Photon Counting Histogram (PCH)
• Raster and Spatio-temporal Image Correlation Spectroscopy (RICS and STICS)
• Single Particle Tracking (SPT)

Globals Software for Spectroscopy and Images

Globals, originally developed in the 1980s at the Laboratory for Fluorescence Dynamics (LFD) for the analysis of multiple files from spectroscopy, is now available for the Windows operating system (Globals for Spectroscopy aka GlobalsWE) and for image analysis using the RICS and phasor approaches (Globals for Images aka SimFCS).

Download Globals

Setup/Installers

• Globals for Images aka SimFCS
  (simfcs-setup.exe: 16.60 MB; October 21, 2009)
• Globals for Spectroscopy
  (globals-setup.exe: 11.79 MB; November 30, 2008)

These are the official installation files of Globals software for the Microsoft Windows XP, Vista, and 7 operating systems. Please read the installation and compatibility notes. Each version may be used free of charge for 30 days. After the 30 days they cannot be used again without purchasing a password.

Latest Executables

• Globals for Images aka SimFCS
  (simfcs.exe: 9.73 MB; November 20, 2009)
• Globals for Spectroscopy
  (globals.exe: 4.56 MB; May 02, 2009)

These executables are updated frequently but may be unstable or not work at all. Use at your own risk: just overwrite the installed executable file with the updated one.

Installation and Compatibility Notes

Requirements

• Operating system: Microsoft Windows XP 32-bit with Service Pack 3 (or compatible).
• Minimum CPU: Pentium IV (Globals for Images) respectively Intel Pentium III (Globals for Spectroscopy) compatible.
• Minimum memory: 1 GB for Globals for Images (2 GB recommended); 256 MB for Globals for Spectroscopy; 512 MB virtual memory.
• Minimum display resolution: 1024x768 pixel.
• OpenGL 2.0 compatible graphics card and display driver (Globals for Images).
• Administrator rights for installation.

Compatibility Issues

Globals software has been reported to run in analysis and simulation mode under Windows 7 and Windows Vista 32-bit and 64-bit, the Wine compatibility layer under Linux, as well as Windows XP running under VirtualPC, VMware Workstation, and the Parallels Desktop for MacOS X. Please note that these configurations are not officially supported and can not be used for data acquisition.

Globals software does not yet fully support non-english language systems, working with non-administrator privileges, any 64-bit Windows systems, or the Windows Vista\7 operating system:

• The installer programs ("globals-setup.exe", "simfcs-setup.exe") must be run with administrator privileges. Either log on as a local administrator before installation or right click the downloaded installer executable and select "Run as" providing administrator credentials. Under Windows Vista\7 the installers will automatically prompt for administrator credentials.
• If Globals software is to be used by non-administrator users, those users must be granted "modify" permissions to the program directories (usually "C:\Program Files\Globals" and "C:\Program Files\SimFCS"). In Windows Explorer, right click on the installation directory and select "Properties" and then the "Security" tab. Select the "Users" group and check the "Allow Modify" permission. This requires administrator privileges. The new installer simfcs-setup.exe will automatically try to change those permissions on the installation directory.
• The operating system must use US decimal symbol (".") and digit grouping (","). These can be set in the "Regional and Language Options" control panel.
• It is recommended to use the "Windows Classic" theme with default scale font size (96 dpi). These can be set in the "Display" respectively "Personalization" control panels.
• In order to use SimFCS in data acquisition mode, devcon.exe must be executed once with administrator privileges. Installation of additional drivers for data acquisition hardware such as Traditional NI-DAQ (Legacy), Becker Hickl TCSPC, ISS cards, and PixeLink might be required. Depending on the type of acquisition hardware, one or more of the following dummy DLL files need to be deleted from the installation directory: nidaq32.dll, PimMegaApi.dll, and spcm32.dll.