Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Tutorials for Globals Software

Tutorials for the Globals Software for Spectroscopy and Images, written by Enrico Gratton and other members of the Laboratory for Fluorescence Dynamics (LFD).

  1. LFD-Globals-Software-Tutorials  [PDF · 72.5 MB · Jan 23, 2017]
  2. pCF cross-correlation  [PDF · 323 KB · Jan 22, 2017]
  3. L-STICS correlation function analysis  [PDF · 710 KB · Sep 26, 2016]
  4. iMSD analysis with scripts  [PDF · 597 KB · Sep 26, 2016]
  5. 2D pair correlation analysis  [PDF · 765 KB · Sep 26, 2016]
  6. Select arbitrary objects in images and save as sub images  [PDF · 280 KB · Jul 10, 2016]
  7. Determining the absolute concentration of NADH  [PDF · 566 KB · Apr 06, 2016]
  8. The Distance program  [PDF · 985 KB · Mar 05, 2016]
  9. Converting very large Zeiss LSM files into BIN files for spectral and 3D analysis  [PDF · 375 KB · Feb 14, 2016]
  10. Pair correlation for images (2D maps and connectivity maps)  [PDF · 610 KB · Sep 20, 2015]
  11. Reading and analyzing FBD files acquired with ISS Vista software  [PDF · 1539 KB · Aug 23, 2015]
  12. Using the correlation of the B carpet  [PDF · 201 KB · Jul 23, 2015]
  13. Brightness-ACF and pCF analysis  [PDF · 238 KB · Jun 30, 2015]
  14. Spectral phasor analysis  [PDF · 329 KB · Jun 18, 2015]
  15. The RICS-scan analysis  [PDF · 430 KB · Jun 14, 2015]
  16. Analyzing tICS using exponentials or phasors  [PDF · 638 KB · May 24, 2015]
  17. Using the hand-draw tool to create masks  [PDF · 505 KB · Mar 17, 2015]
  18. Using PicoQuant PT3 format for FLIM  [PDF · 418 KB · Mar 07, 2015]
  19. The SimFCS64 file formats  [PDF · 209 KB · Nov 30, 2014]
  20. Digital output of the IOtech card  [PDF · 198 KB · Oct 28, 2014]
  21. Reading SDT image files for phasor analysis  [PDF · 510 KB · Oct 19, 2014]
  22. Using masks in the phasor analysis  [PDF · 445 KB · Oct 04, 2014]
  23. The FIT file format  [PDF · 241 KB · Oct 02, 2014]
  24. The iMSD screen and description of the algorithms  [PDF · 1326 KB · Sep 09, 2014]
  25. Reading TIF files for Phasor-FLIM analysis  [PDF · 468 KB · Aug 08, 2014]
  26. Calculation and display of z-phasors  [PDF · 988 KB · Jun 13, 2014]
  27. The compare-next option in the phasor FLIM analysis  [PDF · 312 KB · May 05, 2014]
  28. Using the batch option in the RICS screen  [PDF · 1031 KB · Mar 23, 2014]
  29. Using intensity masks for the FLIM analysis  [PDF · 338 KB · Jan 24, 2014]
  30. Using multiple points in a pixel or multiple lines in a frame for data acquisition using the IOtech 3000 card  [PDF · 242 KB · Jan 09, 2014]
  31. Reading text spectral files and calculate the spectral phasor  [PDF · 203 KB · Nov 03, 2013]
  32. Using the diffusion phasors for the analysis of tICS  [PDF · 568 KB · Oct 13, 2013]
  33. Using the concentration track bar to calculate the concentration of ions or pH in the phasor plot  [PDF · 279 KB · Sep 01, 2013]
  34. N&B analysis using Gaussian cursors  [PDF · 419 KB · Aug 29, 2013]
  35. New file structure for the FLIMbox FBD format  [PDF · 308 KB · Aug 22, 2013]
  36. Code for the trap formula and j-analysis  [PDF · 258 KB · Aug 19, 2013]
  37. Calculating volumes from LSM 3D image stacks  [PDF · 828 KB · Aug 04, 2013]
  38. Deriving the laser frequency from PicoQuant BIN files  [PDF · 465 KB · Aug 03, 2013]
  39. Reading the PicoQuant BIN file  [PDF · 284 KB · Aug 01, 2013]
  40. Using phasors to analyze single point FCS images using the multiple dwell  [PDF · 619 KB · Jun 16, 2013]
  41. Opening PicoqQant line scans in PT3 format  [PDF · 332 KB · Jun 14, 2013]
  42. 3D reconstructions of z-stacks and phasor masks  [PDF · 431 KB · Jun 03, 2013]
  43. Reading SPC files  [PDF · 362 KB · May 30, 2013]
  44. Reading STD file for spectral detector  [PDF · 271 KB · Apr 30, 2013]
  45. GP imaging  [PDF · 490 KB · Apr 30, 2013]
  46. Reading SDT files  [PDF · 311 KB · Apr 29, 2013]
  47. iMSD scan analysis  [PDF · 1041 KB · Mar 31, 2013]
  48. Analyzing TIRF BIN files for iMSD  [PDF · 1035 KB · Mar 30, 2013]
  49. Using separate stage drivers for x-y and z motion  [PDF · 238 KB · Mar 23, 2013]
  50. Changing laser power during the z-stack  [PDF · 225 KB · Mar 23, 2013]
  51. Simulating flow  [PDF · 270 KB · Mar 19, 2013]
  52. New RICS scan-image  [PDF · 929 KB · Mar 09, 2013]
  53. Using the program FORMULA to display 3D surfaces and masks from phasor analysis  [PDF · 208 KB · Jan 15, 2013]
  54. Processing data acquired in the edge tracking mode  [PDF · 346 KB · Dec 11, 2012]
  55. Simulating rotating sticks  [PDF · 264 KB · Nov 25, 2012]
  56. Using the sensitivity parameter in particle tracking  [PDF · 250 KB · Nov 17, 2012]
  57. Reading phosphorescence files  [PDF · 519 KB · Oct 31, 2012]
  58. Correcting photon counts due to detector pile up  [PDF · 313 KB · Oct 22, 2012]
  59. The new scanner waveform  [PDF · 218 KB · Oct 05, 2012]
  60. Using ramps in the tracking screen  [PDF · 261 KB · Jul 23, 2012]
  61. Spectral phasor simulation  [PDF · 366 KB · Jul 15, 2012]
  62. Moving averages columns in the Scanning FCS screen  [PDF · 420 KB · Jun 03, 2012]
  63. Phasor analysis with a heterogeneous reference  [PDF · 1178 KB · Jun 02, 2012]
  64. The IOtech 3001 PCI card  [PDF · 295 KB · May 27, 2012]
  65. Segmentation of trajectories according to the J parameter  [PDF · 580 KB · Apr 29, 2012]
  66. Reading RAW files from the LFD-Lambert acquisition program  [PDF · 212 KB · Apr 20, 2012]
  67. Reading Lambert camera files in RAW format and convert to phasor  [PDF · 269 KB · Apr 15, 2012]
  68. Brightness and number cross-variance  [PDF · 1012 KB · Apr 15, 2012]
  69. Using Becker & Hickl data for phasor analysis  [PDF · 569 KB · Apr 14, 2012]
  70. Calculating the spectral phasor  [PDF · 253 KB · Apr 13, 2012]
  71. Autocorrelation and pair correlation analysis of a line scan  [PDF · 491 KB · Jan 20, 2012]
  72. New intensity scales for the Phasors page  [PDF · 329 KB · Jan 14, 2012]
  73. Using weights in FCS fits  [PDF · 266 KB · Jan 07, 2012]
  74. Reading FLIM line data from the Olympus FV1000  [PDF · 523 KB · Jan 07, 2012]
  75. Dividing the huge vector in two parts when tracking 2 particles simultaneously  [PDF · 381 KB · Dec 20, 2011]
  76. Using a single file for frequency domain fitting of intensity and anisotropy decay  [PDF · 350 KB · Dec 18, 2011]
  77. Using the cell phasor in a time sequence  [PDF · 355 KB · Dec 10, 2011]
  78. Processing FBD files averaging a number of files  [PDF · 202 KB · Dec 06, 2011]
  79. Acquiring a number of frames using the FLIMbox screen  [PDF · 233 KB · Dec 06, 2011]
  80. Using the ISS TDU unit  [PDF · 237 KB · Nov 14, 2011]
  81. Hardware programming of the ISS motor controller box  [PDF · 406 KB · Nov 12, 2011]
  82. BIFL analysis  [PDF · 428 KB · Nov 07, 2011]
  83. Displaying two objects in the modulation tracking screen  [PDF · 492 KB · Nov 05, 2011]
  84. The BIFL page  [PDF · 353 KB · Oct 31, 2011]
  85. Changes to the track screen  [PDF · 261 KB · Aug 24, 2011]
  86. Calibrating galvo and piezo  [PDF · 283 KB · Aug 21, 2011]
  87. Calibrating the phase and amplitude of the scanner to compensate for its slow response at high frequency  [PDF · 306 KB · Aug 01, 2011]
  88. Processing FLIM line files  [PDF · 370 KB · Jul 12, 2011]
  89. Function readthePICOQUANTfile  [PDF · 243 KB · Jun 14, 2011]
  90. Analyzing N&B in AVI movies  [PDF · 283 KB · Jun 13, 2011]
  91. Analyzing FCS and PCH raw data from the Zeiss Confocor 2  [PDF · 336 KB · Jun 09, 2011]
  92. FLIM-FRET phasor analysis of FBD files  [PDF · 699 KB · Jun 02, 2011]
  93. Extracting ramp segments in MT nanoimaging  [PDF · 329 KB · May 28, 2011]
  94. Decoding the FBD file  [PDF · 266 KB · May 21, 2011]
  95. FLIM measurements using the Lambert camera  [PDF · 232 KB · Apr 04, 2011]
  96. Using the Ti Sapphire laser and the FLIMbox  [PDF · 238 KB · Mar 05, 2011]
  97. Fitting time domain anisotropy data in Globals for Spectroscopy  [PDF · 383 KB · Jan 15, 2011]
  98. Calibrating the time response of the scanner  [PDF · 262 KB · Jan 10, 2011]
  99. Using the FLIMbox with the image acquire screen  [PDF · 204 KB · Dec 17, 2010]
  100. Using linked cursors in the Phasor plot and obtain the histogram of fractional intensities  [PDF · 263 KB · Dec 12, 2010]
  101. Straighten up lines in scanning FCS  [PDF · 321 KB · Dec 08, 2010]
  102. Analyzing single point time-time domain data using phasors  [PDF · 405 KB · Dec 01, 2010]
  103. Reading PicoQuant PT3 files for FCS and lifetime FCS  [PDF · 438 KB · Oct 29, 2010]
  104. Analyzing anisotropy decay using associative models in Globals for Spectroscopy  [PDF · 391 KB · Oct 22, 2010]
  105. Simulating cylinders of elliptical shape for 3D tracking  [PDF · 453 KB · Oct 04, 2010]
  106. Simulating actin flow in cell protrusion  [PDF · 311 KB · Oct 04, 2010]
  107. Functions in the Scanning FCS analysis page for the 3D reconstruction using modulation tracking  [PDF · 308 KB · Oct 04, 2010]
  108. Assessing the effect of background counts on the determination of the correct radius of a cylinder using the MT method  [PDF · 327 KB · Oct 04, 2010]
  109. Merging images in the RICS and scanning FCS screen  [PDF · 232 KB · Oct 02, 2010]
  110. Analyzing cell protrusions using Modulation Tracking  [PDF · 542 KB · Sep 06, 2010]
  111. Simulating regions of different diffusion for testing pCF  [PDF · 1366 KB · Sep 03, 2010]
  112. Reading and processing image frames larger than 256 in the FLIMbox screen  [PDF · 1090 KB · Aug 08, 2010]
  113. Determining the laser repetition rate for the FLIMbox when using the TiSapphire laser  [PDF · 494 KB · Aug 01, 2010]
  114. Reading FCS files (ISS format) for FCS and images. RICS and N&B with the Leica SP5  [PDF · 885 KB · Jul 04, 2010]
  115. Measure MSD using correlation  [PDF · 390 KB · Jun 17, 2010]
  116. Reading M format ASCII files in Globals  [PDF · 669 KB · May 16, 2010]
  117. Reading BIN files from the Olympus TIRF  [PDF · 580 KB · Feb 19, 2010]
  118. GP analysis of TIRF dual view images  [PDF · 402 KB · Feb 19, 2010]
  119. F-FCS, Lifetime resolved FCS using the FLIMbox card  [PDF · 348 KB · Feb 14, 2010]
  120. Calibrating the 3-axis card after January 15, 2010  [PDF · 308 KB · Jan 15, 2010]
  121. Tracking surfaces (villi) in 3D  [PDF · 279 KB · Jan 03, 2010]
  122. Tracking surfaces in xz and yz planes  [PDF · 219 KB · Dec 30, 2009]
  123. Analyzing TIRF data  [PDF · 737 KB · Dec 30, 2009]
  124. FLIMbox connections  [PDF · 237 KB · Oct 27, 2009]
  125. Simplified alignment procedures for the ALBA system  [PDF · 238 KB · Oct 25, 2009]
  126. BIFL analysis  [PDF · 911 KB · Oct 25, 2009]
  127. External clock for synchronization  [PDF · 315 KB · Oct 24, 2009]
  128. Improvements to GP analysis  [PDF · 502 KB · Sep 24, 2009]
  129. Calculating and displaying GP images and histograms  [PDF · 484 KB · Sep 20, 2009]
  130. Code for the N&B functions  [PDF · 290 KB · Jul 20, 2009]
  131. Nanometer resolution Circular Tracking (nrCT)  [PDF · 1291 KB · Jul 17, 2009]
  132. Extracting lines in an image for pair correlation analysis  [PDF · 222 KB · Jul 05, 2009]
  133. Fitting autocorrelation curves using the anomalous diffusion term  [PDF · 314 KB · Jul 02, 2009]
  134. Selecting ROI in the phasor analysis  [PDF · 1231 KB · Jun 23, 2009]
  135. Saving mask and histogram images in the phasor screen  [PDF · 352 KB · Jun 23, 2009]
  136. Exporting N&B primitives for further display and analysis  [PDF · 271 KB · Jun 19, 2009]
  137. Installing the FLIMbox in a new computer  [PDF · 204 KB · Jun 08, 2009]
  138. The multiple-cursor selector for phasors and multiple files processing  [PDF · 279 KB · May 26, 2009]
  139. FLIM with multiple channels  [PDF · 214 KB · Mar 16, 2009]
  140. Temporal image correlation spectroscopy (tICS)  [PDF · 326 KB · Feb 08, 2009]
  141. GP images selection using the FRET screen  [PDF · 318 KB · Feb 05, 2009]
  142. The FLIMbox as of January 27, 2009  [PDF · 375 KB · Jan 27, 2009]
  143. Calibrating the ALBA box  [PDF · 394 KB · Dec 15, 2008]
  144. Phasor analysis  [PDF · 676 KB · Dec 02, 2008]
  145. Reading PicoQuant BIN files  [PDF · 440 KB · Nov 18, 2008]
  146. The FLIMbox as of November 11, 2008  [PDF · 219 KB · Nov 11, 2008]
  147. N&B analysis using the analog mode in the LSM510  [PDF · 523 KB · Oct 23, 2008]
  148. Particle tracking for images  [PDF · 320 KB · Oct 22, 2008]
  149. FRET intensity image calculations  [PDF · 357 KB · Oct 22, 2008]
  150. Using the ISS ALBA with the two-photon laser  [PDF · 227 KB · Oct 12, 2008]
  151. Using the FLIMbox with the ISS ALBA microscope  [PDF · 258 KB · Oct 12, 2008]
  152. Reading Lambert instrument files  [PDF · 614 KB · Sep 28, 2008]
  153. Reading PixeLINK camera files in RAW format  [PDF · 244 KB · Aug 19, 2008]
  154. Cross-N&B  [PDF · 809 KB · Apr 21, 2008]
  155. ALBA box commands  [PDF · 236 KB · Apr 14, 2008]
  156. Using frame freezing for FLIMbox acquisition with the Zeiss 510  [PDF · 331 KB · Apr 13, 2008]
  157. Using the SCAN function in the RICS page  [PDF · 605 KB · Mar 31, 2008]
  158. GP image displays in the RICS screen  [PDF · 274 KB · Mar 19, 2008]
  159. RICS cross-correlation  [PDF · 191 KB · Mar 18, 2008]
  160. Reading RAW files in the RICS screen  [PDF · 211 KB · Feb 26, 2008]
  161. RICS cross-correlation analysis  [PDF · 198 KB · Feb 21, 2008]
  162. Using the Zeiss Confocor 3 with the FLIMbox  [PDF · 510 KB · Feb 01, 2008]
  163. Particle Tracking  [PDF · 1111 KB · Oct 16, 2007]
  164. Using the tracking routine with an image stack  [PDF · 220 KB · Sep 18, 2007]
  165. Using calibration equations in FLIM  [PDF · 239 KB · Sep 02, 2007]
  166. Concepts in Fluorescence Correlation Spectroscopy  [PDF · 364 KB · Jun 26, 2007]
  167. Simulating FCS and particle trajectories  [PDF · 327 KB · Mar 18, 2007]
  168. Phasor autofluorescence subtraction  [PDF · 268 KB · Feb 01, 2007]
  169. Line scan analysis  [PDF · 198 KB · Nov 06, 2006]
  170. N&B analysis for the line scan of Paxillin-EGFP  [PDF · 284 KB · Oct 12, 2006]
  171. GP image analysis  [PDF · 314 KB · Aug 17, 2006]
  172. The brightness and N button in the RICS screen  [PDF · 606 KB · Jul 01, 2006]
  173. FLIM-FRET phasor analysis of files from the Lambert instrument  [PDF · 440 KB · Jun 27, 2006]
  174. The time derivative and velocity buttons  [PDF · 382 KB · Jun 05, 2006]
  175. Calibrating the PSF volume for PCH analysis  [PDF · 220 KB · Jan 06, 2001]