Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Book Chapters

Book chapters authored or coauthored by members of the Laboratory for Fluorescence Dynamics (LFD) from 1987 to present. Access to most PDF documents is password protected due to copyright restrictions. Reprints can be requested from the authors.

2016

  1. Gratton E.
    Measurements of fluorescence decay time by the frequency domain method.
    Perspectives on Fluorescence: A Tribute to Gregorio Weber (Springer Series on Fluorescence). By DM Jameson (Editors). Springer Berlin Heidelberg, 2016.
  2. Cardarelli F Gratton E.
    Spatiotemporal fluorescence correlation spectroscopy of inert tracers: a journey within cells, one molecule at a time.
    Perspectives on Fluorescence: A Tribute to Gregorio Weber (Springer Series on Fluorescence). By DM Jameson (Editors). Springer Berlin Heidelberg, 2016.

2014

  1. Gratton E Digman MA.
    Laurdan identifies different lipid membranes in eukaryotic cells.
    Cell Membrane Nanodomains: From Biochemistry to Nanoscopy. By A Cambi and DS Lidke (Editors). CRC Press, pp. 283-304, 2014. ISBN: 9781482209891
  2. Digman MA.
    Fluctuation spectroscopy methods for the analysis of membrane processes.
    Cell Membrane Nanodomains: From Biochemistry to Nanoscopy. By A Cambi and DS Lidke (Editors). CRC Press, pp. 215-238, 2014. ISBN: 9781482209891
  3. Golfetto O, Hinde E, Gratton E.
    The Laurdan spectral phasor method to explore membrane micro-heterogeneity and lipid domains in live cells.
    Methods in Membrane Lipids (Methods in Molecular Biology, Vol. 1232). By DM Owen (Editors). Springer New York, pp. 273-290, 2014. ISBN: 9781493917518
  4. Digman MA Gratton E.
    The phasor approach to fluorescence lifetime imaging: exploiting phasor linear properties.
    Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. By L Marcu, PMW French, and DS Elson (Editors). CRC Press, pp. 235-248, 2014. ISBN: 9781439861677

2013

  1. Gratton E, Stakic M, Digman MA.
    Raster image correlation spectroscopy and number and brightness analysis.
    Fluorescence Fluctuation Spectroscopy (FFS) Part A (Methods in Enzymology, Vol. 518). By SY Tetin (Editors). Academic Press, pp. 121-144, 2013. ISBN: 9780123884220

2010

  1. Levi V Gratton E.
    Three-dimensional particle tracking in a laser scanning fluorescence microscope.
    Single-particle tracking and single molecule energy transfer. By C Bräuchle, DC Lamb, and J Michaelis (Editors). Wiley VCH, pp. 3-24, 2010. ISBN: 9783527322961

2009

  1. Gratton E, Toronov VY, Wolf U, Wolf M.
    Detection of brain activity by near-infrared light.
    Biomedical Optical Imaging. By JG Fujimoto and D Farkas (Editors). Oxford University Press, pp. 378-395, 2009. ISBN: 9780195150445

2007

  1. Sánchez SA, Tricerri MA, Gunther G, Gratton E.
    Laurdan generalized polarization: from cuvette to microscope.
    Modern Research and Educational Topics in Microscopy. Vol. 3: Applications in Physical/Chemical Sciences (Microscopy Book Series, Vol. 3). By A Méndez-Vilas and J Díaz (Editors). Formatex Research Center, Badajoz, Spain, pp. 1007-1014, 2007. ISBN: 9788461194209
  2. Celli A, Lee CY, Gratton E.
    Fluorescence microscopy to study pressure between lipids in giant unilamellar vesicles.
    Methods in Membrane Lipids (Methods in Molecular Biology, Vol. 400). By AM Dopico (Editors). Humana Press, pp. 333-340, 2007. ISBN: 9781588296627
  3. Digman MA Gratton E.
    The RICS method: measurement of fast dynamics in cells with the laser scanning microscope.
    Image Analysis in Medical Microscopy and Pathology. By HS Wu and AJ Einstein (Editors). Research Signpost, Kerala, India, pp. 207-224, 2007. ISBN: 8130801167

2006

  1. Gratton E vandeVen MJ.
    Laser sources for confocal microscopy.
    The Handbook of Biological Confocal Microscopy (Third Edition). By JB Pawley (Editors). Springer-Verlag New York, pp. 80-125, 2006. ISBN: 9780387259215
  2. Gratton E, Breusegem SY, Barry NP, Ruan Q, Eid JS.
    Fluctuation correlation spectroscopy in cells: determination of molecular aggregation.
    Biophotonics: Optical Science and Engineering for the 21st Century (Vol. XVIII). By X Shen and R van Wijk (Editors). Springer Verlag, pp. 1-14, 2006. ISBN: 9780387249957

2005

  1. Redford GI Clegg RM.
    Real-time fluorescence lifetime imaging and FRET using fast gated image intensifiers.
    Molecular Imaging: FRET Microscopy and Spectroscopy (Methods in Physiology Series). By A Periasamy and RN Day (Editors). Academic Press, pp. 193-226, 2005. ISBN: 9780195177206
  2. Clegg RM.
    Nuts and bolts of excitation energy migration and energy transfer.
    Chlorophyll a Fluorescence: A Signature of Photosynthesis (Advances in Photosynthesis and Respiration, Vol. 19). By GC Papageorgiou and Govindjee (Editors). Springer, pp. 83-105, 2005. ISBN: 9781402032172
  3. Hazlett TL, Ruan Q, Tetin SY.
    Application of fluorescence correlation spectroscopy to hapten-antibody binding.
    Protein-Ligand Interactions: Methods and Applications (Methods in Molecular Biology, Vol. 305). By GU Nienhaus (Editors). pp. 415-438, 2005. ISBN: 9781588293725
  4. Gratton E Fantini S.
    Reflectance and transmittance spectroscopy.
    Laser and Current Optical Techniques in Biology (Comprehensive Series in Photochemical & Photobiological Sciences). By G Palumbo and R Pratesi (Editors). Royal Society of Chemistry, pp. 211-258, 2005. ISBN: 9780854043217
  5. Hanson KM Clegg RM.
    Two-photon fluorescence imaging and reactive oxygen species detection within the epidermis.
    Epidermal Cells: Methods and Protocols (Methods in Molecular Biology, Vol. 289). By K Turksen (Editors). Humana Press, pp. 413-421, 2005. ISBN: 9781592598304

2004

  1. Palumbo G, Pratesi R, Gratton E, Fantini S, Häder DP, Jori G.
    Reflectance and transmittance spectroscopy.
    Lasers and Current Optical Techniques in Biology (Comprehensive Series in Photochemical & Photobiological Sciences). By G Palumbo and R Pratesi (Editors). Royal Society of Chemistry, pp. 213-247, 2004. ISBN: 9780854043217

2003

  1. Dong CY, French TE, So PTC, Buehler C, Berland KM, Gratton E.
    Fluorescence-lifetime imaging techniques for microscopy.
    Digital Microscopy. A second edition of "Video Microscopy" (Methods in Cell Biology, Vol. 72). By G Sluder and DE Wolf (Editors). Academic Press, pp. 431-464, 2003. ISBN: 9780125641692
  2. Beechem JM, Gratton E, Ameloot M, Knutson JR, Brand L.
    The global analysis of fluorescence intensity and anisotropy decay data: second-generation theory and programs.
    Topics in Fluorescence Spectroscopy: "Principles" (Vol. 2). By JR Lakowicz (Editors). Springer US, pp. 241-305, 2003. ISBN: 9780306438752
  3. Müller JD, Chen Y, Gratton E.
    Fluorescence correlation spectroscopy.
    Biophotonics, Part B (Methods in Enzymology, Vol. 361). By G Marriott and I Parker (Editors). Academic Press, pp. 69-92, 2003. ISBN: 9780121822644
  4. Bagatolli LA, Sánchez SA, Hazlett TL, Gratton E.
    Giant vesicles, Laurdan, and two-photon fluorescence microscopy: evidence of lipid lateral separation in bilayers.
    Biophotonics, Part A (Methods in Enzymology, Vol. 360). By G Marriott and I Parker (Editors). Academic Press, pp. 481-500, 2003. ISBN: 9780121822637
  5. Clegg RM, Holub O, Gohlke C.
    Fluorescence lifetime-resolved imaging: measuring lifetimes in an image.
    Biophotonics, Part A (Methods in Enzymology, Vol. 360). By G Marriott and I Parker (Editors). Academic Press, pp. 509-42, 2003. ISBN: 9780121822637
  6. Bell JD, Sánchez SA, Hazlett TL.
    Liposomes in the study of phospholipase A2 activity.
    Liposomes, Part B (Methods in Enzymology, Vol. 372). By N Duzgunes (Editors). Academic Press, pp. 19-48, 2003. ISBN: 9780121822750

2002

  1. Tramier M, Holub O, Croney JC, Ishii T, Seifried SE, Jameson DM.
    Binding of ethidium to yeast tRNA(Phe): a new perspective on an old Bromide.
    Fluorescence Spectroscopy, Imaging and Probes. By R Kraayenhof, AJWG Visser, and HC Gerritsen (Editors). Springer-Verlag Berlin, Heidelberg, New York, pp. 111-121, 2002. ISBN: 9783540427681
  2. Learmonth RP Gratton E.
    Assessment of membrane fluidity in individual yeast cells by Laurdan Generalized Polarization and multi-photon scanning fluorescence microscopy.
    Fluorescence Spectroscopy, Imaging and Probes (Vol. 2). By R Kraayenhof, AJWG Visser, and HC Gerritsen (Editors). Springer-Verlag Berlin, Heidelberg, New York, pp. 241-252, 2002. ISBN: 9783540427681

2001

  1. Dong CY, Buehler C, So PTC, French T, Gratton E.
    Biological applications of time-resolved, pump-probe fluorescence microscopy and spectroscopy in the frequency domain.
    Methods In Cellular Imaging (Methods in Physiology Series, Vol. IV). By A Periasamy (Editors). Springer New York, pp. 324-340, 2001. ISBN: 9780195139365
  2. Parasassi T, Bagatolli LA, Gratton E, Levi M, Ursini F, Yu W, Zajicek HK.
    Two-photon excitation microscopy for image spectroscopy and biochemistry of tissues, cells, organelles and lipid vesicles under physiological conditions.
    Confocal and Two-Photon Microscopy: Foundations, Applications and Advances. By A Diaspro (Editors). John Wiley & Sons, New York, pp. 469-482, 2001. ISBN: 9780471409205
  3. Chen Y, Müller JD, Eid JS, Gratton E.
    Two-photon fluorescence fluctuation spectroscopy.
    New Trends in Fluorescence Spectroscopy:Applications to Chemical and Life Sciences (Springer Series on Fluorescence). By B Valeur and JC Brochon (Editors). pp. 277-296, 2001. ISBN: 9783540677796
  4. Müller JD, Chen Y, Gratton E.
    Photon counting histogram statistics.
    Fluorescence Correlation Spectroscopy. Theory and Applications (Springer Series in Chemical Physics, Vol. 65). By R Riegler and EL Elson (Editors). Springer-Verlag, Berlin, pp. 410-437, 2001. ISBN: 9783540674337
  5. Breusegem SY, Loontiens FG, Regenfuss P, Clegg RM.
    Kinetics of binding of Hoechst dyes to DNA studied by stopped-flow fluorescence techniques.
    Drug-Nucleic Acid Interactions (Methods in Enzymology, Vol. 340). Academic Press, pp. 212-33, 2001. ISBN: 9780121822415

2000

  1. Stühmeier F, Clegg RM, Hillisch A, Diekmann S.
    Practical aspects of fluorescence resonance energy transfer (FRET) and its applications in nucleic acid biochemistry.
    DNA-Protein Interactions: A Practical Approach. By A Travers and M Buckle (Editors). Oxford University Press, pp. 77-94, 2000. ISBN: 9780199636914
  2. Yu W, Mantulin WW, Gratton E.
    Fluorescence lifetime imaging: new microscopy technologies.
    Emerging Tools for Single-Cell Analysis (Advances in Optical Measurement Technologies). By G Durack and JP Robinson (Editors). Wiley-Liss, pp. 139-173, 2000. ISBN: 9780471315759
  3. Dong CY, Kim KH, Buehler C, Hsu L, Kim H, So PTC, Masters BR, Gratton E, Kochevar IE.
    Probing deep-tissue structures by two-photon fluorescence microscopy.
    Emerging Tools for Single-Cell Analysis (Advances in Optical Measurement Technologies). By G Durack and JP Robinson (Editors). Wiley-Liss, Inc, pp. 221-237, 2000. ISBN: 9780471315759

1999

  1. Masters BR, So PTC, Gratton E.
    Multiphoton excitation microscopy and spectroscopy of cells, tissues, and human skin in vivo.
    Fluorescent and Luminescent Probes for Biological Activity: A Practical Guide to Technology for Quantitative Real-Time Analysis (Second Edition) (Biological Techniques Series). By WT Mason (Editors). Academic Press, pp. 414-432, 1999. ISBN: 9780124778306
  2. Masters BR, So PTC, Kim KH, Buehler C, Gratton E.
    Multiphoton excitation microscopy, confocal microscopy, and spectroscopy of living cells and tissues; functional metabolic imaging of human skin in vivo.
    Confocal Microscopy (Methods in Enzymology, Vol. 307). By JN Abelson, MI Simon, and PM Conn (Editors). Academic Press, pp. 513-36, 1999. ISBN: 0121822087

1998

  1. French TE, So PTC, Dong CY, Berland KM, Gratton E.
    Fluorescence lifetime imaging techniques for microscopy.
    Video Microscopy (Methods in Cell Biology, Vol. 56). By G Sluder and DE Wolf (Editors). Academic Press, pp. 277-304, 1998. ISBN: 9780126491609
  2. Masters BR, So PTC, Gratton E.
    Multiphoton excitation microscopy of in vivo human skin. Functional and morphological optical biopsy based on three-dimensional imaging, lifetime measurements and fluorescence spectroscopy.
    Advances in Optical Biopsy and Optical Mammography (Annals of the New York Academy of Sciences, Vol. 838). pp. 58-67, 1998. ISBN: 9781573311267

1997

  1. So PTC, Dong CY, Berland KM, French TE, Gratton E.
    Time-resolved stimulated-emission and transient-absorption microscopy and spectroscopy.
    Nonlinear and Two-Photon-Induced Fluorescence (Topics in Fluorescence Spectroscopy, Vol. 5). By JR Lakowicz (Editors). Plenum Press, New York, pp. 427-469, 1997. ISBN: 9780306455537
  2. Gratton G, Fabiani M, Corballis PM, Gratton E.
    Noninvasive detection of fast signals from the cortex using frequency-domain optical methods.
    Imaging Brain Structure and Function: Emerging Technologies in the Neurosciences (Annals of the New York Academy of Sciences, Vol. 820). By DS Lester, CC Felder, and EN Lewis (Editors). pp. 286-298, 1997. ISBN: 9781573310697

1996

  1. So PTC, French TE, Yu W, Berland KM, Dong CY, Gratton E.
    Two-photon fluorescence microscopy: time-resolved and intensity imaging.
    Fluorescence Imaging Spectroscopy and Microscopy (Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications. Vol. 137). By XF Wang and B Herman (Editors). John Wiley & Sons, pp. 351-374, 1996. ISBN: 9780471015277

1995

  1. Gratton E vandeVen MJ.
    Laser sources for confocal microscopy.
    The Handbook of Biological Confocal Microscopy (Second Edition). By JB Pawley (Editors). Springer-Verlag New York, pp. 69-97, 1995. ISBN: 0306448262

1992

  1. vandeVen MJ Gratton E.
    Time-resolved fluorescence lifetime imaging.
    Optical Microscopy: Emerging Methods and Applications. By B Herman and JJ Lemasters (Editors). Academic Press, pp. 373-402, 1992. ISBN: 9780123420602
  2. Ferreira ST Gratton E.
    Enzyme activity in reverse micelles: roles of diffusion and extent of hydration.
    Biomolecules in Organic Solvents. By A Gómez-Puyou (Editors). CRC Press, pp. 189-202, 1992. ISBN: 9780849348235

1990

  1. Gratton E, Alcala JR, Barbieri BB.
    Frequency-domain fluorometry.
    Luminescence Techniques in Chemical and Biochemical Analysis (Luminescence Spectroscopy, Vol. 12). By WRG Baeyens, D de Keukeleire, and K Korkidis (Editors). CRC Press, pp. 47-72, 1990. ISBN: 9780824783693

1989

  1. Gratton E vandeVen MJ.
    Laser sources for confocal microscopy.
    The Handbook of Biological Confocal Microscopy. By JB Pawley (Editors). Springer-Verlag New York, pp. 47-59, 1989. ISBN: 0306448262

1988

  1. Gratton E, Silva N Jr, Ferreira ST.
    Fluorescence of tryptophan and protein dynamics.
    Biological and Artificial Intelligence Systems. By E Clementi and S Chin (Editors). ESCOM Science Publishers, Leiden, The Netherlands, pp. 49-55, 1988. ISBN: 9789072199027

1986

  1. Careri G Gratton E.
    The statistical time correlation approach to enzyme action: the role of hydration.
    The Fluctuating Enzyme (Nonequilibrium Problems in the Physical Sciences & Biology). By GR Welch (Editors). John Wiley & Sons, New York, pp. 227-262, 1986. ISBN: 9780471888703