Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Publications by Robert M Clegg (1945-2012)

2014

  1. Eichorst JP, Teng KW, Clegg RM.
    Fluorescence lifetime imaging techniques: Frequency-domain FLIM.
    Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics. By L Marcu, PMW French, and DS Elson (Editors). CRC Press, pp. 165-186, 2014. ISBN: 9781439861677

2013

  1. Eichorst JP, Teng KW, Clegg RM.
    Polar plot representation of time-resolved fluorescence.
    Fluorescence Spectroscopy and Microscopy (Methods in Molecular Biology, Vol. 1076). By Y Engelborghs and AJWG Visser (Editors). Humana Press, pp. 97-112, 2014.

2012

  1. Eichorst JP, Clegg RM, Wang Y.
    Red-shifted fluorescent proteins monitor enzymatic activity in live HT-1080 cells with fluorescence lifetime imaging microscopy (FLIM).
    J Microsc. 2012; 248(1): 77-89. PMCID: PMC3872149
  2. Brown G, Butler PJ, Chang DW, Chien S, Clegg RM, Dewey CF, Dong C, Guo XE, Helmke BP, Hess H, Jacobs CR, Kaunas RR, Kumar S, Lu HH, Mathur AB, van Mow C, Schmid-Schönbein GW, Skoracki R, Wang N, Wang Y, Zhu C.
    Cellular and molecular bioengineering: a tipping point.
    Cell Mol Bioeng. 2012; 5(3): 239-253. PMCID: PMC3525706
  3. Chen YC, Spring BQ, Clegg RM.
    Fluorescence lifetime imaging comes of age how to do it and how to interpret it.
    Spectroscopic Methods of Analysis Methods and Protocols (Methods in Molecular Biology, Vol. 875). By WM Bujalowski (Editors). pp. 1-22, 2012.
  4. Periasamy A, Vogel SS, Clegg RM.
    FRET 65: A celebration of Förster.
    J Biomed Opt. 2012; 17(1): 011001.

2011

  1. Chen YC Clegg RM.
    Spectral resolution in conjunction with polar plots improves the accuracy and reliability of FLIM measurements and estimates of FRET efficiency.
    J Microsc. 2011; 244(1): 21-37.
  2. Matsubara S, Chen YC, Caliandro R, Govindjee, Clegg RM.
    Photosystem II fluorescence lifetime imaging in avocado leaves: Contributions of the lutein-epoxide and violaxanthin cycles to fluorescence quenching.
    J Photochem Photobiol B. 2011; 104(1-2): 271-284.
  3. Eichorst JP, Huang H, Clegg RM, Wang Y.
    Phase differential enhancement of FLIM to distinguish FRET components of a biosensor for monitoring molecular activity of membrane Type 1 matrix metalloproteinase in live cells.
    J Fluoresc. 2011; 21(4): 1763-1777. PMCID: PMC3637990
  4. Clegg RM.
    Bioassays, FLIM and FRET.
    8th International Weber Symposium. Kauai, Hawaii. June 12-17, 2011.
  5. Kolossov VL, Spring BQ, Clegg RM, Henry JJ, Sokolowski A, Kenis PJA, Gaskins HR.
    Development of a high-dynamic range, GFP-based FRET probe sensitive to oxidative microenvironments.
    Exp Biol Med (Maywood). 2011; 236(6): 681-691. PMCID: PMC3158092

2009

  1. Chen YC Clegg RM.
    Fluorescence lifetime-resolved imaging.
    Photosynth Res. 2009; 102(2-3): 143-155.
  2. Noomnarm U Clegg RM.
    Fluorescence lifetimes: fundamentals and interpretations.
    Photosynth Res. 2009; 101(2-3): 181-194.
  3. Spring BQ Clegg RM.
    Image analysis for denoising full-field frequency-domain fluorescence lifetime images.
    J Microsc. 2009; 235(2): 221-237.
  4. Clegg RM.
    Fluorescence lifetime-resolved imaging: what, why, how - a prologue.
    FLIM Microscopy in Biology and Medicine. By A Periasamy and RM Clegg (Editors). Chapman & Hall/CRC, pp. 3-34, 2009. ISBN: 9781420078909
  5. Spring BQ Clegg RM.
    Frequency-domain FLIM.
    FLIM Microscopy in Biology and Medicine. By A Periasamy and RM Clegg (Editors). Chapman & Hall/CRC, pp. 115-142, 2009. ISBN: 9781420078909
  6. Chen YC, Spring BQ, Buranachai C, Tong B, Malachowski GC, Clegg RM.
    General concerns of FLIM data representation and analysis: frequency-domain model-free analysis.
    FLIM Microscopy in Biology and Medicine. By A Periasamy and RM Clegg (Editors). Chapman & Hall/CRC, pp. 291-339, 2009. ISBN: 9781420078909
  7. Periasamy A Clegg RM.
    FLIM applications in the biomedical sciences.
    FLIM Microscopy in Biology and Medicine. By A Periasamy and RM Clegg (Editors). Chapman & Hall/CRC, pp. 385-400, 2009. ISBN: 9781420078909
  8. Chen YC, Spring BQ, Buranachai C, Malachowski GC, Clegg RM.
    What is behind all those lifetimes anyway, and where do we go from here?.
    BiOS 2009. Part of SPIE Photonics West. San Jose, California, January 24-29, 2009.
    Multiphoton Microscopy in the Biomedical Sciences IX (Proceedings of SPIE, Vol. 7183). By A Periasamy and PTC So (Editors). 7183-1, 2009. ISBN: 9780819474292
  9. Clegg RM.
    Förster Resonance Energy Transfer - FRET what is it, why do it, and how it's done.
    FRET and FLIM Techniques (Laboratory Techniques in Biochemistry and Molecular Biology, Vol. 33). By TWJ Gadella (Editors). Elsevier Science, pp. 1-57, 2009. ISBN: 9780080549583

2008

  1. Vámosi G Clegg RM.
    Helix-coil transition of a four-way DNA junction observed by multiple fluorescence parameters.
    J Phys Chem B. 2008; 112(41): 13136-13148.
  2. Buranachai C, Kamiyama D, Chiba A, Williams BD, Clegg RM.
    Rapid frequency-domain FLIM spinning disk confocal microscope: lifetime resolution, image improvement and wavelet analysis.
    J Fluoresc. 2008; 18(5): 929-942.
  3. Kolossov VL, Spring BQ, Sokolowski A, Conour JE, Clegg RM, Kenis PJA, Gaskins HR.
    Engineering redox-sensitive linkers for genetically encoded FRET-based biosensors.
    Exp Biol Med. 2008; 233(2): 238-248.

2007

  1. Malachowski GC, Clegg RM, Redford GI.
    Analytic solutions to modelling exponential and harmonic functions using Chebyshev polynomials: fitting frequency-domain lifetime images with photobleaching.
    J Microsc. 2007; 228(3): 282-295.
  2. Spring BQ Clegg RM.
    Fluorescence measurements of duplex DNA oligomers under conditions conducive for forming M-DNA (a metal-DNA complex).
    J Phys Chem B. 2007; 111(33): 10040 -10052.
  3. Ermolenko DN, Spiegel PC, Majumdar ZK, Hickerson RP, Clegg RM, Noller HF.
    The antibiotic viomycin traps the ribosome in an intermediate state of translocation.
    Nat Struct Mol Biol. 2007; 14: 493-497.
  4. Holub O, Seufferheld MJ, Gohlke C, Govindjee, Heiss GJ, Clegg RM.
    Fluorescence lifetime imaging microscopy of chlamydomonas reinhardtii: non-photochemical quenching mutants and the effect of photosynthetic inhibitors on the slow chlorophyll fluorescence transient.
    J Microsc. 2007; 226(2): 90-120.
  5. Ermolenko DN, Majumdar ZK, Hickerson RP, Spiegel PC, Clegg RM, Noller HF.
    Observation of intersubunit movement of the ribosome in solution using FRET.
    J Mol Biol. 2007; 370(3): 530-540.
  6. Clegg RM.
    The history of FRET: from conception through the labors of birth.
    Reviews in Fluorescence (Vol. 2006). By JR Lakowicz and CD Geddes (Editors). Springer, New York, pp. 1-45, 2007. ISBN: 9780387293424

2006

  1. Clegg RM.
    A simple derivation of the luminescence anisotropy decay from randomly distributed cylinders rotating about a single axis.
    J Fluoresc. 2006; 16(6): 761-771.
  2. Chandler DE, Majumdar ZK, Heiss GJ, Clegg RM.
    Ruby crystal for demonstrating time- and frequency-domain methods of fluorescence lifetime measurements.
    J Fluoresc. 2006; 16(6): 793-807.
  3. Spring BQ Clegg RM.
    Microsecond mixing with polarization and spectral resolution for fast reaction kinetics.
    50th Annual Meeting of the Biophysical Society, Salt Lake City, Utah, 2006.
    Biophys J. 2006; Suppl, 693-Pos/B559.
  4. Noomnarm U, Sutin JDB, Clegg RM.
    Repetitive pressure-jump for relaxation kinetics.
    50th Annual Meeting of the Biophysical Society, Salt Lake City, Utah, 2006.
    Biophys J. 2006; Suppl, 692-Pos/B558.

2005

  1. Redford GI, Majumdar ZK, Sutin JDB, Clegg RM.
    Properties of microfluidic turbulent mixing revealed by fluorescence lifetime imaging.
    J Chem Phys. 2005; 123(22): 224504.
  2. Majumdar ZK, Sutin JDB, Clegg RM.
    Microfabricated continuous-flow, turbulent, microsecond mixer.
    Rev Sci Instrum. 2005; 76(12): 125103.
  3. Hickerson RP, Majumdar ZK, Baucom A, Clegg RM, Noller HF.
    Measurement of internal movements within the 30 S ribosomal subunit using Förster resonance energy transfer.
    J Mol Biol. 2005; 354(2): 459-72.
  4. Eckhoff DA, Sutin JDB, Clegg RM, Gratton E, Rogozhina EV, Braun PV.
    Optical characterization of ultrasmall Si nanoparticles prepared through electrochemical dispersion of bulk Si.
    J Phys Chem B. 2005; 109(42): 19786-97.
  5. Majumdar ZK, Hickerson RP, Noller HF, Clegg RM.
    Measurements of internal distance changes of the 30S ribosome using FRET with multiple donor-acceptor pairs: quantitative spectroscopic methods.
    J Mol Biol. 2005; 351(5): 1123-45.
  6. Redford GI Clegg RM.
    Polar plot representation for frequency-domain analysis of fluorescence lifetimes.
    J Fluoresc. 2005; 15(5): 805-15.
  7. Redford GI Clegg RM.
    Real-time fluorescence lifetime imaging and FRET using fast gated image intensifiers.
    Molecular Imaging: FRET Microscopy and Spectroscopy (Methods in Physiology Series). By A Periasamy and RN Day (Editors). Academic Press, pp. 193-226, 2005. ISBN: 9780195177206
  8. Clegg RM, Breusegem SY, Barry NP, Holub O, Govindjee, Redford GI.
    How can one choose the best method for measuring FRET in a microscope with my biological system?.
    Microscopy and Microanalysis 2005. Honolulu, Hawaii, USA, July 31-August 4, 2005.
    Microsc Microanal. 2005; 11(Suppl 2).
  9. Majumdar ZK, Hickerson R, Noller HF, Clegg RM.
    Measurements of internal distance changes of the 30s ribosome using FRET with multiple donor-acceptor pairs: quantitative spectroscopic methods.
    6th International Weber Symposium. Kauai, Hawaii. July 22-28, 2005.
  10. Clegg RM.
    Nuts and bolts of excitation energy migration and energy transfer.
    Chlorophyll a Fluorescence: A Signature of Photosynthesis (Advances in Photosynthesis and Respiration, Vol. 19). By GC Papageorgiou and Govindjee (Editors). Springer, pp. 83-105, 2005. ISBN: 9781402032172
  11. Spring BQ Clegg RM.
    Enhanced acceptor FRET facilitated by M-DNA.
    49th Annual Meeting of the Biophysical Society, Long Beach, California, 2005.
    Biophys J. 2005; Suppl, 799-Pos/B638.
  12. Buranachai C, Ha T, Clegg RM.
    Single molecule study of dynamic characteristics of Tetramethyrhodamine-DNA.
    49th Annual Meeting of the Biophysical Society, Long Beach, California, 2005.
    Biophys J. 2005; Suppl, 1705-Pos/B691.
  13. Majumdar ZK, Clegg RM, Noller HF, Hickerson RP.
    Monitoring internal movements of the 30S E. Coli Ribosome with quantitative and kinetic FRET measurements.
    49th Annual Meeting of the Biophysical Society, Long Beach, California, 2005.
    Biophys J. 2005; Suppl, 1981-Pos/B93.
  14. Hanson KM Clegg RM.
    Two-photon fluorescence imaging and reactive oxygen species detection within the epidermis.
    Epidermal Cells: Methods and Protocols (Methods in Molecular Biology, Vol. 289). By K Turksen (Editors). Humana Press, pp. 413-421, 2005. ISBN: 9781592598304

2004

  1. Rao S, Sutin JDB, Clegg RM, Gratton E, Nayfeh MH, Habbal S, Tsolakidis A, Martin RM.
    Excited states of tetrahedral single-core Si29 nanoparticles.
    Phys Rev B. 2004; 69(20): 205319.
  2. Hanson KM, Hayden PJ, Kubilus J, Clegg RM.
    Detecting reactive oxygen species in skin using two-photon fluorescence imaging microscopy.
    65th Annual Meeting of the Society for Investigative Dermatology. Providence, RI. April 28-May 1, 2004.
    J Invest Dermatol. 2004; 122(3): A140, 839.
  3. Buranachai C, Leuba S, Ordahl CP, Kun E, Clegg RM.
    Histone H1 and DNA interaction study using several methods in fluorescence spectroscopy.
    48th Annual Meeting of the Biophysical Society, Baltimore, Maryland, 2004.
    Biophys J. 2004; Suppl, 3055-Pos/B307.
  4. Spring BQ Clegg RM.
    Measuring enhanced acceptor fluorescence as a probe for electron transfer in m-DNA.
    48th Annual Meeting of the Biophysical Society, Baltimore, Maryland, 2004.
    Biophys J. 2004; Suppl, 2443-Pos/B540.
  5. Majumdar ZK, Clegg RM, Noller HF, Hickerson RP.
    Visualizing ribosomal movements with Förster resonance energy transfer.
    48th Annual Meeting of the Biophysical Society, Baltimore, Maryland, 2004.
    Biophys J. 2004; Suppl, 1644-Pos/B629.
  6. Eckhoff DA, Sutin JDB, Rogozhina EV, Stuart JN, Sweedler JV, Braun PV, Clegg RM, Gratton E.
    Effects of oxidation on the photoluminescence from silicon nanoparticles.
    48th Annual Meeting of the Biophysical Society, Baltimore, Maryland, 2004.
    Biophys J. 2004; Suppl, 3156-Pos/B409.
  7. Hohng S, Wilson TJ, Tan E, Clegg RM, Lilley DMJ, Ha T.
    Conformational flexibility of four-way junctions in RNA.
    J Mol Biol. 2004; 336(1): 69-79.
  8. McKinney SA, Tan E, Wilson TJ, Nahas MK, Déclais AC, Clegg RM, Lilley DMJ, Ha T.
    Single-molecule studies of DNA and RNA four-way junctions.
    Nucleic Acids Chemistry and Biology: The 5th Cambridge Symposium, Queen's College, Cambridge, 31 August–3 September 2003.
    Biochem Soc Trans. 2004; 32(1): 41-45.
  9. Clegg RM.
    The vital contributions of Perrin and Förster.
    Biophotonics Int. 2004; 11(9): 42-45.

2003

  1. Hanson KM Clegg RM.
    Bioconvertible vitamin antioxidants improve sunscreen photoprotection against UV-induced reactive oxygen species.
    J Cosmet Sci. 2003; 54(5): 589-98.
  2. Tan E, Wilson TJ, Nahas MK, Clegg RM, Lilley DMJ, Ha T.
    A four-way junction accelerates hairpin ribozyme folding via a discrete intermediate.
    Proc Natl Acad Sci USA. 2003; 100(16): 9308-13. PMCID: PMC170914
  3. Behne MJ, Barry NP, Hanson KM, Aronchik I, Clegg RM, Gratton E, Feingold K, Holleran WM, Elias PM, Mauro TM.
    Neonatal development of the stratum corneum pH gradient: localization and mechanisms leading to emergence of optimal barrier function.
    J Invest Dermatol. 2003; 120(6): 998-1006.
  4. Redford GI, Gohlke C, Clegg RM.
    Versatile rapid lifetime imaging.
    47th Annual Meeting of the Biophysical Society, San Antonio, Texas, 2003.
    Biophys J. 2003; 84(2): 584, 2860-Pos/B459.
  5. Majumdar ZK, Sutin JDB, Redford GI, Clegg RM.
    Microsecond kinetics in a continuous flow turbulent mixer - detection with fluorescence intensity and fluorescence lifetime imaging.
    47th Annual Meeting of the Biophysical Society, San Antonio, Texas, 2003.
    Biophys J. 2003; 84(2), 2321-Pos/B697.
  6. Breusegem SY, Mackinnon AC, Barry NP, Lin X, Gratton E, Williams BD, Clegg RM.
    In vivo visualization of protein-protein interactions in C. Elegans muscle attachment structures by FRET microscopy.
    47th Annual Meeting of the Biophysical Society, San Antonio, Texas, 2003.
    Biophys J. 2003; 84(2), 105-Plat.
  7. Tan E, Nahas MK, Wilson TJ, Clegg RM, Lilley DMJ, Ha T.
    Dynamics of four-way junction enhances the active site formation of hairpin ribozyme.
    47th Annual Meeting of the Biophysical Society, San Antonio, Texas, 2003.
    Biophys J. 2003; 84(2), 888-Pos/B140.
  8. Clegg RM, Holub O, Gohlke C.
    Fluorescence lifetime-resolved imaging: measuring lifetimes in an image.
    Biophotonics, Part A (Methods in Enzymology, Vol. 360). By G Marriott and I Parker (Editors). Academic Press, pp. 509-42, 2003. ISBN: 9780121822637

2002

  1. Behne MJ, Meyer JW, Hanson KM, Barry NP, Murata S, Crumrine D, Clegg RM, Gratton E, Holleran WM, Elias PM, Mauro TM.
    NHE1 regulates the stratum corneum permeability barrier homeostasis. Microenvironment acidification assessed with fluorescence lifetime imaging.
    J Biol Chem. 2002; 277(49): 47399-406.
  2. Hanson KM, Behne MJ, Barry NP, Mauro TM, Gratton E, Clegg RM.
    Two-photon fluorescence lifetime imaging of the skin stratum corneum pH gradient.
    Biophys J. 2002; 83(3): 1682-90. PMCID: PMC1302264
  3. Hanson KM Clegg RM.
    Observation and quantification of ultraviolet-induced reactive oxygen species in ex vivo human skin.
    Photochem Photobiol. 2002; 76(1): 57-63.
  4. Breusegem SY, Barry NP, Lin X, Gratton E, Clegg RM, Williams BD.
    Protein interactions in C. Elegans muscle attachment structures studied in vivo by fluorescence resonance energy transfer (FRET).
    Midwest Worm Meeting. June 28-30, 2002. Washington University in St. Louis.
  5. Hanson KM, Holub O, Gohlke C, Barry NP, Behne MJ, Gratton E, Clegg RM.
    Imaging in skin and plants: using photons and fluorescence lifetimes to find the molecules and quantify the information.
    8th Biophysical Society Annual Meeting and Frontier Biophysics Symposium, Taiwan, ROC, 2002.
  6. Behne MJ, Barry NP, Hanson KM, Meyer JW, Crumrine D, Clegg RM, Gratton E, Holleran WM, Elias PM, Mauro TM.
    NHE1 regulates stratum corneum acidification and permeability barrier homeostasis: identification of acidic microenvironments with FLIM.
    Biomedical Topical Meeting, Miami Beach, Florida, 2002.
  7. Barry NP, Hanson KM, Gratton E, Clegg RM, Behne MJ, Mauro TM.
    Applications of ultrafast lasers to two-photon fluorescence and lifetime imaging.
    Commercial and Biomedical Applications of Ultrafast and Free-Electron Lasers (Proceedings of SPIE, Vol. 4633). By GS Edwards, J Neev, A Ostendorf, and JC Sutherland (Editors). pp. 50-61, 2002.
  8. Breusegem SY, Barry NP, Mackinnon AC, Lin X, Williams BD, Gratton E, Clegg RM.
    Protein interactions in C. elegans muscle attachment structures studied in vivo by fluorescence resonance energy transfer (FRET).
    46th Annual Meeting of the Biophysical Society, San Francisco, California, 2002.
    Biophys J. 2002; 82(1): 491e.
  9. Hanson KM, Barry NP, Behne MJ, Mauro TM, Gratton E, Clegg RM.
    Two-photon fluorescence lifetime imaging of the skin's stratum corneum pH gradient.
    46th Annual Meeting of the Biophysical Society, San Francisco, California, 2002.
    Biophys J. 2002; 82(1): 494c.
  10. vandeVen MJ, Holub O, Gohlke C, Govindjee, Valcke R, Ameloot M, Clegg RM.
    Fast macroscopic chlorophyll fluorescence lifetime imaging of apple fruit skin.
    46th Annual Meeting of the Biophysical Society, San Francisco, California, 2002.
    Biophys J. 2002; 82(1): 502a.
  11. Behne MJ, Hanson KM, Barry NP, Clegg RM, Gratton E, Holleran WM, Elias PM, Mauro TM.
    Correlation of extracellular enzyme activity and pH in Stratum Corneum by fluorescence lifetime imaging.
    46th Annual Meeting of the Biophysical Society, San Francisco, California, 2002.
    Biophys J. 2002; 82(1): 501a.
  12. Majumdar ZK, Sutin JDB, Clegg RM.
    A continuous flow, turbulent mixer for studying fast reaction kinetics.
    46th Annual Meeting of the Biophysical Society, San Francisco, California, 2002.
    Biophys J. 2002; 82(1): 483d.
  13. Breusegem SY, Clegg RM, Loontiens FG.
    Base-sequence specificity of Hoechst 33258 and DAPI binding to five (A/T)4 DNA sites with kinetic evidence for more than one high-affinity Hoechst 33258-AATT complex.
    J Mol Biol. 2002; 315(5): 1049-61.
  14. Clegg RM.
    FRET tells us about proximities, distances, orientations and dynamic properties.
    J Biotechnol. 2002; 82(3): 177-9.

2001

  1. Breusegem SY, Sadat-Ebrahimi SE, Douglas KT, Bichenkova EV, Clegg RM, Loontiens FG.
    Experimental precedent for the need to involve the primary hydration layer of DNA in lead drug design.
    J Med Chem. 2001; 44(16): 2503-6.
  2. Breusegem SY, Sadat-Ebrahimi SE, Douglas KT, Clegg RM, Loontiens FG.
    Increased stability and lifetime of the complex formed between DNA and meta-phenyl-substituted Hoechst dyes as studied by fluorescence titrations and stopped-flow kinetics.
    J Mol Biol. 2001; 308(4): 649-63.
  3. Breusegem SY, Barry NP, Mackinnon AC, Williams BD, Clegg RM.
    Integrin interactions in C. Elegans muscle studied in vivo by fluorescence resonance energy transfer (FRET).
    45th Annual Meeting of the Biophysical Society, Boston, Massachusetts, 2001.
    Biophys J. 2001; 80(1 Pt 2): 162a, 656.2-Pos.
  4. Gohlke C, Holub O, Clegg RM.
    FliFast: Software for fast fluorescence lifetime-resolved image acquisition with concurrent analysis and visual feedback.
    45th Annual Meeting of the Biophysical Society, Boston, Massachusetts, 2001.
    Biophys J. 2001; 80(1 Pt 2): 169a, 656.54-Pos.
  5. Holub O, Seufferheld MJ, Gohlke C, Govindjee, Clegg RM.
    Application of real-time fluorescence lifetime-resolved imaging in photosynthesis: studies of maize leaves (Zea Mays), small mustard leaves (Arabidopsis Thaliana), and of individual wild-type and mutant cells of the green alga Chlamydomonas Reinhardtii.
    45th Annual Meeting of the Biophysical Society, Boston, Massachusetts, 2001.
    Biophys J. 2001; 80(1 Pt 2): 428a, 1819-Pos.
  6. Majumdar ZK, D’Amico ML, Clegg RM.
    Fluorescent Properties of Cy5 (5-N-N’-diethyltetramethylindodicarbocyanine) covalently linked to DNA.
    45th Annual Meeting of the Biophysical Society, Boston, Massachusetts, 2001.
    Biophys J. 2001; 80(1 Pt 2): 483a, 2066-Pos.
  7. Tan E Clegg RM.
    Conformational behavior of DNA-Cy3 complex.
    45th Annual Meeting of the Biophysical Society, Boston, Massachusetts, 2001.
    Biophys J. 2001; 80(1 Pt 2): 483a, 2065-Pos.
  8. Breusegem SY, Loontiens FG, Regenfuss P, Clegg RM.
    Kinetics of binding of Hoechst dyes to DNA studied by stopped-flow fluorescence techniques.
    Drug-Nucleic Acid Interactions (Methods in Enzymology, Vol. 340). Academic Press, pp. 212-33, 2001. ISBN: 9780121822415

2000

  1. Holub O, Seufferheld MJ, Gohlke C, Govindjee, Clegg RM.
    Fluorescence lifetime imaging (FLI) in real-time - a new technique in photosynthesis research.
    Photosynthetica. 2000; 8(4): 581-599.
  2. Stühmeier F, Hillisch A, Clegg RM, Diekmann S.
    Fluorescence energy transfer analysis of DNA structures containing several bulges and their interaction with CAP.
    J Mol Biol. 2000; 302(5): 1081-100.
  3. Stühmeier F, Clegg RM, Hillisch A, Diekmann S.
    Practical aspects of fluorescence resonance energy transfer (FRET) and its applications in nucleic acid biochemistry.
    DNA-Protein Interactions: A Practical Approach. By A Travers and M Buckle (Editors). Oxford University Press, pp. 77-94, 2000. ISBN: 9780199636914
  4. Clegg RM, Stühmeier F, Gohlke C, Vámosi G.
    Conformations and stabilities of nucleic acids using FRET.
    Abstr Paper Am Chem Soc Natl Meet. 2000; 220: U239-U239 568-PHYS Part 2.
  5. Holub O, Seufferheld MJ, Gohlke C, Clegg RM, Govindjee.
    Real-time fluorescence lifetime-resolved images of individual cells of wild type and NPQ mutants of Chlamydomonas reinhardtii.
    9th International Conference on the Cell and Molecular Biology of Chlamydamonas, Amsterdam, The Netherlands, May 21-26, 2000.
  6. Holub O, Gohlke C, Clegg RM.
    Want to know something about your fluorescent samples no lenses can resolve? Big scale scanning setup for real-time fluorescence lifetime-resolved imaging.
    44th Annual Meeting of the Biophysical Society, New Orleans, Louisiana, 2000.
    Biophys J. 2000; 78(1 Pt 2), 1464-Pos.
  7. Hanson KM, Barry NP, Gratton E, Clegg RM.
    Fluorescence lifetime imaging of pH in the stratum corneum.
    44th Annual Meeting of the Biophysical Society, New Orleans, Louisiana, 2000.
    Biophys J. 2000; 78(1 Pt 2), 1478-Pos.
  8. Breusegem SY, Loontiens FG, Clegg RM.
    Binding of Hoechst 33258 and DAPI to (A/T)4 DNA sites: base-sequence specificity determined by fluorescence titrations and stopped flow kinetics.
    44th Annual Meeting of the Biophysical Society, New Orleans, Louisiana, 2000.
    Biophys J. 2000; 78(1 Pt 2), 1804-Pos.

1999

  1. Clegg RM.
    A glimpse of nucleic acid conformations through the eyes of fluorescence.
    4th International Weber Symposium. Maui, Hawaii. June 23-27, 1999.
  2. Breusegem SY, Loontiens FG, Clegg RM.
    Binding kinetics of Hoechst 33258 and some of its derivatives to DNA.
    43rd Annual Meeting of the Biophysical Society, Baltimore, Maryland, 1999.
    Biophys J. 1999; 76(1 Pt 2).
  3. Stühmeier F, Hillisch A, Diekmann S, Clegg RM.
    Fluorescence resonance energy transfer analysis of DNS-structures containing several A5 bulges and their interaction with proteins.
    43rd Annual Meeting of the Biophysical Society, Baltimore, Maryland, 1999.
    Biophys J. 1999; 76(1 Pt 2).
  4. Holub O Clegg RM.
    "Second order" sequence specificity of DAPI-binding to AT-regions in DNA.
    43rd Annual Meeting of the Biophysical Society, Baltimore, Maryland, 1999.
    Biophys J. 1999; 76(1 Pt 2).

1998

  1. Vámosi G Clegg RM.
    The helix-coil transition of DNA duplexes and hairpins observed by multiple fluorescence parameters.
    Biochemistry. 1998; 37(40): 14300-16.
  2. Erijman L Clegg RM.
    Reversible stalling of transcription elongation complexes by high pressure.
    Biophys J. 1998; 75(1): 453-62. PMCID: PMC1299718
  3. Holub O Clegg RM.
    Stereochemistry of DNA molecules by homo-energy transfer.
    32nd Annual Meeting of the Biophysical Society, Kansas City, Missouri, 1998.
    Biophys J. 1998; 74(2 Pt 2): A288, W-Pos192.
  4. Schneider PC, Holub O, Clegg RM.
    Real-time fluorescence lifetime-resolved images of 5-aminolevulinic acid (ALA)-induced Protoporphyrin IX (PPIX) in cells.
    32nd Annual Meeting of the Biophysical Society, Kansas City, Missouri, 1998.
    Biophys J. 1998; 74(2 Pt 2), Tu-Pos250.

1997

  1. Bassi GS, Murchie AIH, Walter F, Clegg RM, Lilley DMJ.
    Ion-induced folding of the hammerhead ribozyme: a fluorescence resonance energy transfer study.
    EMBO J. 1997; 16(24): 7481-9.
  2. Stühmeier F, Lilley DMJ, Clegg RM.
    Effect of additional unpaired bases on the stability of three-way DNA junctions studied by fluorescence techniques.
    Biochemistry. 1997; 36(44): 13539-51.
  3. Stühmeier F, Welch JB, Murchie AIH, Lilley DMJ, Clegg RM.
    Global structure of three-way DNA junctions with and without additional unpaired bases: a fluorescence resonance energy transfer analysis.
    Biochemistry. 1997; 36(44): 13530-8.
  4. Schneider PC Clegg RM.
    Rapid acquisition, analysis, and display of fluorescence lifetime-resolved images for real-time applications.
    Rev Sci Instrum. 1997; 68(11): 4107-4119.
  5. Duckett DR, Murchie AIH, Clegg RM, Bassi GS, Giraud-Panis MJE, Lilley DMJ.
    Nucleic acid structure and recognition.
    Biophys Chem. 1997; 68(1-3): 53-62.
  6. Schneider PC, Holub O, Clegg RM.
    Real-time fluorescence lifetime-resolved imaging.
    Second Conference on Fluorescence Microscopy & Fluorescent Probes. April 9-12, 1997. Prague, Czechia.
  7. Holub O Clegg RM.
    Stereochemistry of DNA molecules by homo-energy transfer.
    Second Conference on Fluorescence Microscopy & Fluorescent Probes. April 9-12, 1997. Prague, Czechia.
  8. Erijman L, Villas-Boas M, Clegg RM.
    The effect of high pressure on protein-DNA interactions: nucleosomes and RNA polymerase enzymatic activity.
    22nd Annual Lorne Conference On Protein Structure And Function. February 9-13, 1997. Lorne, Australia.

1996

  1. Clegg RM, Schneider PC, Jovin TM.
    Fluorescence lifetime-resolved imaging microscopy.
    Biomedical Optical Instrumentation and Laser-assisted Biotechnology. Erice, Italy. Nov 10-22, 1995.
    Biomedical Optical Instrumentation and Laser-assisted Biotechnology (NATO Science Series E, Vol. 325). By AMV Scheggi, S Martellucci, AN Chester, and R Pratesi (Editors). Kluwer Academic Press, pp. 143-156, 1996. ISBN: 0792341724
  2. Vámosi G, Gohlke C, Clegg RM.
    Photophysics of 5-carboxytetramethylrhodamine linked to the 5'-end of ss & ds DNA molecules.
    XIIth International Biophysics Congress, Amsterdam, The Netherlands, August 11-16, 1996.
    Prog Biophys Mol Biol. 1996; 65(Suppl 1): 76, P-B1-31.
  3. Stühmeier F, Welch JB, Lilley DMJ, Clegg RM.
    Fluorescence measurements of DNA three-way junctions.
    XIIth International Biophysics Congress, Amsterdam, The Netherlands, August 11-16, 1996.
    Prog Biophys Mol Biol. 1996; 65(Suppl 1): 131, P-B1-29.
  4. Erijman L Clegg RM.
    Transcriptional inhibition by high pressure in vitro.
    XIIth International Biophysics Congress, Amsterdam, The Netherlands, August 11-16, 1996.
    Prog Biophys Mol Biol. 1996; 65(Suppl 1): 82, P-B2-10.
  5. Vámosi G, Gohlke C, Clegg RM.
    Fluorescence characteristics of 5-carboxytetramethylrhodamine linked covalently to the 5' end of oligonucleotides: multiple conformers of single-stranded and double-stranded dye-DNA complexes.
    Biophys J. 1996; 71(2): 972-94. PMCID: PMC1233554
  6. Schneider PC, Gadella TWJ Jr, Jovin TM, Clegg RM.
    Analytica. April 23-25 1996, Munich, Germany.
  7. Clegg RM.
    Fluorescence resonance energy transfer.
    Fluorescence Imaging Spectroscopy and Microscopy (Chemical Analysis: A Series of Monographs on Analytical Chemistry and Its Applications). By XF Wang and B Herman (Editors). John Wiley & Sons, pp. 179-252, 1996. ISBN: 9780471015277
  8. Erijman L Clegg RM.
    High pressure electrophoresis in narrow bore glass tubes: one- and two-dimensional separations of protein subunits.
    Rev Sci Instrum. 1996; 67(3): 813-817.
  9. Clegg RM Schneider PC.
    Fluorescence lifetime-resolved imaging microscopy: a general description of the lifetime-resolved imaging measurements.
    Based on the proceedings of a conference held in Prague, Czech Republic, June 25-28, 1995.
    Fluorescence Microscopy and Fluorescent Probes. By J Slavik (Editors). Plenum Press, New York, pp. 15-33, 1996. ISBN: 0306453924
  10. Gadella TWJ Jr, van Hoek A, Visser AJWG, Schneider PC, Jovin TM, Clegg RM.
    Frequency-domain fluorescence lifetime imaging microscopy: methodology and examples.
    Scanning. 1996; 18(1): 59-60.

1995

  1. Erijman L Clegg RM.
    Heterogeneity of E. coli RNA polymerase revealed by high pressure.
    J Mol Biol. 1995; 253(2): 259-65.
  2. Lilley DMJ, Clegg RM, Diekmann S, Seeman NC, von Kitzing E, Hagerman PJ.
    A nomenclature of junctions and branchpoints in nucleic acids.
    Nucleic Acids Res. 1995; 23(17): 3363-3364.
  3. Arndt-Jovin DJ, Clegg RM, Jovin TM.
    Intracellular pH and ion distributions in living cells determined by phase-detected fluorescence lifetime imaging, FLIM.
    3rd International Weber Symposium. Maui, Hawaii. July 30-August 2, 1995.
  4. Jurkiewicz E, Villas-Boas M, Silva JL, Weber G, Hunsmann G, Clegg RM.
    Inactivation of simian immunodeficiency virus by hydrostatic pressure.
    Proc Natl Acad Sci USA. 1995; 92(15): 6935-7. PMCID: PMC41445
  5. Lilley DMJ, Clegg RM, Diekmann S, Seeman NC, von Kitzing E, Hagerman PJ.
    Nomenclature Committee of the International Union of Biochemistry and Molecular Biology (NC-IUBMB). A nomenclature of junctions and branchpoints in nucleic acids. Recommendations 1994.
    Eur J Biochem. 1995; 230(1): 1-2.
  6. Clegg RM.
    Fluorescence resonance energy transfer.
    Curr Opin Biotechnol. 1995; 6(1): 103-110.

1994

  1. Gohlke C, Murchie AIH, Lilley DMJ, Clegg RM.
    Kinking of DNA and RNA helices by bulged nucleotides observed by fluorescence resonance energy transfer.
    Proc Natl Acad Sci USA. 1994; 91(24): 11660-4. PMCID: PMC45291
  2. Gadella TWJ Jr, Clegg RM, Jovin TM.
    Fluorescence lifetime imaging microscopy: pixel-by-pixel analysis of phase-modulation data.
    Bioimaging. 1994; 2(3): 139-159.
  3. Clegg RM, Gadella TWJ Jr, Jovin TM.
    Lifetime-resolved fluorescence imaging.
    Time-Resolved Laser Spectroscopy in Biochemistry IV (Proceedings of SPIE, Vol. 2137). By JR Lakowicz (Editors). pp. 105-118, 1994.
  4. Clegg RM, Murchie AIH, Lilley DMJ.
    The solution structure of the four-way DNA junction at low-salt conditions: a fluorescence resonance energy transfer analysis.
    Biophys J. 1994; 66(1): 99-109. PMCID: PMC1275668

1993

  1. Gadella TWJ Jr, Jovin TM, Clegg RM.
    Fluorescence lifetime imaging microscopy (FLIM): spatial resolution of microstructures on the nanosecond time scale.
    Biophys Chem. 1993; 48(2): 221-239.
  2. Vámosi G, Gohlke C, Murchie AIH, Lilley DMJ, Clegg RM.
    Probing the conformation of DNA structures - 4-way junctions and bulges - with fluorescence.
    11th International Biophysics Congress and IUPAB General Assembly at Budapest, Hungary, July 25-30,1993.
  3. Lilley DMJ Clegg RM.
    The structure of the four-way junction in DNA.
    Annu Rev Biophys Biomol Struct. 1993; 22: 299-328.
  4. Villas-Boas M, Silva JL, Clegg RM.
    Pressure studies on protein-DNA interaction.
    NATO Advanced Study Institute. Aquafredda di Maratea, Italy. September 20-October 3, 1992.
    High Pressure Chemistry, Biochemistry and Materials Sciences (NATO Science Series C, Vol. 401). By R Winter and J Jonas (Editors). Kluwer Academic Press, pp. 579-602, 1993. ISBN: 9780792322900
  5. Lilley DMJ Clegg RM.
    The structure of branched DNA species.
    Q Rev Biophys. 1993; 26(2): 131-175.
  6. Clegg RM, Murchie AIH, Lilley DMJ.
    The four-way DNA junction: a fluorescence resonance energy transfer study.
    Braz J Med Biol Res. 1993; 26(4): 405-16.
  7. Clegg RM, Murchie AIH, Zechel A, Lilley DMJ.
    Observing the helical geometry of double-stranded DNA in solution by fluorescence resonance energy transfer.
    Proc Natl Acad Sci USA. 1993; 90(7): 2994-8. PMCID: PMC46223

1992

  1. Clegg RM.
    Fluorescence resonance energy transfer and nucleic acids.
    DNA Structures Part A: Synthesis and Physical Analysis of DNA (Methods in Enzymology, Vol. 211). Academic Press, pp. 353-388, 1992. ISBN: 0121821129
  2. Duckett DR, Murchie AIH, Bhattacharyya A, Clegg RM, Diekmann S, von Kitzing E, Lilley DMJ.
    The structure of DNA junctions and their interaction with enzymes.
    Eur J Biochem. 1992; 207(1): 285-95.
  3. Clegg RM, Murchie AIH, Zechel A, Carlberg C, Diekmann S, Lilley DMJ.
    Fluorescence resonance energy transfer analysis of the structure of the four-way DNA junction.
    Biochemistry. 1992; 31(20): 4846-56.
  4. Clegg RM, Feddersen BA, Gratton E, Jovin TM.
    Time-resolved imaging fluorescence microscopy.
    SPIE Conference, Los Angeles, California, 1990.
    Time-Resolved Laser Spectroscopy in Biochemistry III (Proceedings of SPIE, Vol. 1640). By JR Lakowicz (Editors). pp. 448-460, 1992.
  5. Clegg RM, Marriott GM, Federson BA, Gratton E, Arndt-Jovin DJ, Jovin TM.
    Time resolved image microscopy.
    36th Annual Meeting of the Biophysical Society, Houston, Texas, 9-13 February 1992.
    Biophys J. 1992; 61(2 Pt 2): A415, 2393.
  6. Feddersen BA, Gratton E, Clegg RM.
    Binary solvent studies of Hoechst 33258.
    36th Annual Meeting of the Biophysical Society, Houston, Texas, 9-13 February 1992.
    Biophys J. 1992; 61(2 Pt 2): A180, 1030.

1991

  1. Marriott GM, Clegg RM, Arndt-Jovin DJ, Jovin TM.
    Time resolved imaging microscopy. Phosphorescence and delayed fluorescence imaging.
    Biophys J. 1991; 60(6): 1374-87. PMCID: PMC1260198
  2. Clegg RM Arndt-Jovin DJ.
    Spectroscopic analysis of AO bound to RNA and DNA.
    XV Congress of the International Society for Analytical Cytology Bergen, Norway, August 25-30, 1991.
    Cytometry. 1991; 12(Suppl 5): 103, 549C.
  3. Feddersen BA, Gratton E, Clegg RM, Jovin TM.
    An optical and electronic heterodyning technique for use with CCD cameras and array detectors for time-resolved fluorescence with sub-nanosecond resolution.
    35th Annual Meeting of the Biophysical Society, San Francisco, California, February 1991.
    Biophys J. 1991; 59(2 Pt 2): 156a, MI-Po405.
  4. Loontiens FG, McLaughlin LW, Diekmann S, Clegg RM.
    Binding of Hoechst 33258 and 4',6'-diamidino-2-phenylindole to self-complementary decadeoxynucleotides with modified exocyclic base substituents.
    Biochemistry. 1991; 30(1): 182-9.

1990

  1. Loontiens FG, Regenfuss P, Zechel A, Dumortier L, Clegg RM.
    Binding characteristics of Hoechst 33258 with calf thymus DNA, poly[d(A-T)], and d(CCGGAATTCCGG): multiple stoichiometries and determination of tight binding with a wide spectrum of site affinities.
    Biochemistry. 1990; 29(38): 9029-39.
  2. Duckett DR, Murchie AIH, Clegg RM, Zechel A, von Kitzing E, Diekmann S, Lilley DMJ.
    The structure of the Holliday junction.
    Structure & Methods: Human Genome Initiative & DNA Recombination. By RH Sarma (Editors). Adenine Pr, pp. 157-181, 1990. ISBN: 9780940030299
  3. Jovin TM, Arndt-Jovin DJ, Marriott GM, Clegg RM, Robert-Nicoud M, Schormann T.
    Distance, wavelength and time: the versatile 3rd dimensions in light emission microscopy.
    International Conference on Video Microscopy. June 4-7, 1989. Chapel Hill, North Carolina.
    Optical Microscopy for Biology. By B Herman and K Jacobson (Editors). Wiley-Liss, New York, pp. 575-602, 1990. ISBN: 9780471567622
  4. Clegg RM, Marriott GM, Feddersen BA, Gratton E, Jovin TM.
    Sensitive and rapid determinations of fluorescence lifetimes in the frequency domain in a light microscope.
    34th Annual Meeting of the Biophysical Society, Baltimore, Maryland, February 1990.
    Biophys J. 1990; 57(2 Pt 2): 375a, Tu-Pos574.

1989

  1. Murchie AIH, Clegg RM, von Kitzing E, Duckett DR, Diekmann S, Lilley DMJ.
    Fluorescence energy transfer shows that the four-way DNA junction is a right-handed cross of antiparallel molecules.
    Nature. 1989; 341(6244): 763-6.
  2. Piston DW, Marriott GM, Radivoyevich T, Clegg RM, Jovin TM, Gratton E.
    Wide-band acousto-optic light modulator for frequency domain fluorometry and phosphorimetry.
    Rev Sci Instrum. 1989; 60(8): 2596-2600.
  3. Jovin TM, Arndt-Jovin DJ, Robert-Nicauld M, Schormann T, Marriott GM, Clegg RM.
    Luminescence digital imaging microscopy.
    33rd Annual Meeting of the Biophysical Society, Cincinnati, Ohio 1989.
    Biophys J. 1989; 55(2 Pt 2): 432a, W-PM-Sym-2.
  4. Jovin TM, Marriott GM, Clegg RM, Arndt-Jovin DJ.
    Photophysical processes exploited in digital imaging microscopy: fluorescence resonance energy transfer and delayed luminescence.
    Ber Bunsen Phys Chem. 1989; 93(3): 387-391.

1988

  1. van de Sande JH, Ramsing NB, Germann MW, Elhorst W, Kalisch BW, von Kitzing E, Pon RT, Clegg RM, Jovin TM.
    Parallel stranded DNA.
    Science. 1988; 241(4865): 551-557.

1987

  1. MacGregor RB Jr Clegg RM.
    Ethidium-DNA binding kinetics in an agarose gel.
    Biopolymers. 1987; 26(12): 2103-6.
  2. MacGregor RB Jr, Clegg RM, Jovin TM.
    Viscosity dependence of ethidium-DNA intercalation kinetics.
    Biochemistry. 1987; 26(13): 4008-16.
  3. Regenfuss P Clegg RM.
    Diffusion-controlled association of a dye, 1-anilinonaphthalene-8-sulfonic acid, to a protein, bovine serum albumin, using a fast-flow microsecond mixer and stopped-flow.
    Biophys Chem. 1987; 26(1): 83-9.

1986

  1. Clegg RM.
    Derivation of diffusion controlled chemical rate constants with the help of Einstein's original derivation of the diffusion constant.
    J Chem Ed. 1986; 63(7): 571-574.

1985

  1. MacGregor RB Jr, Clegg RM, Jovin TM.
    Pressure-jump study of the kinetics of ethidium bromide binding to DNA.
    Biochemistry. 1985; 24(20): 5503-10.
  2. Loontiens FG, de Boeck H, Clegg RM.
    Physicochemical aspects of carbohydrate binding to some plant lectins with binding preference for N-acetylgalactosamine and galactose.
    J Biosci. 1985; 8(1-2): 425-436.
  3. Clegg RM Vaz WL.
    Translational diffusion of proteins and lipids in artificial lipid bilayer membranes. A comparison of experiment with theory.
    Progress in Protein-Lipid Interactions (Progress in Protein-Lipid Interactions, Vol. 1). By A Watts (Editors). Elsevier Science Ltd, pp. 173-229, 1985. ISBN: 9780444806307
  4. de Boeck H, MacGregor RB Jr, Clegg RM, Sharon N, Loontiens FG.
    Binding of N-dansylgalactosamine to the lectin from Erythrina cristagalli as followed by stopped-flow and pressure-jump relaxation kinetics.
    Eur J Biochem. 1985; 149(1): 141-5.
  5. Vaz WL, Hallmann D, Clegg RM, Gambacorta A, de Rosa M.
    A comparison of the translational diffusion of a normal and a membrane-spanning lipid in L alpha phase 1-palmitoyl-2-oleoylphosphatidylcholine bilayers.
    Eur Biophys J. 1985; 12(1): 19-24.
  6. Regenfuss P, Clegg RM, Fulwyler MJ, Barrantes FJ, Jovin TM.
    Mixing liquids in microseconds.
    Rev Sci Instrum. 1985; 56(2): 283-290.
  7. Vaz WL, Clegg RM, Hallmann D.
    Translational diffusion of lipids in liquid crystalline phase phosphatidylcholine multibilayers. A comparison of experiment with theory.
    Biochemistry. 1985; 24(3): 781-6.

1984

  1. Clegg RM.
    Qualitative exact diagnostic tests for two common relaxation kinetic models.
    J Chem Phys. 1984; 81(12): 5546 -5551.

1983

  1. Loontiens FG, Clegg RM, van Landschoot A.
    Some physicochemical aspects of oligosaccharide binding to Concanavalin A and wheat germ agglutinin.
    J Biosci. 1983; 5(Suppl 1): 105-120.
  2. Clegg RM, Loontiens FG, Sharon N, Jovin TM.
    Dynamic evidence for an extended subsite structure of the ligand combining site on wheat germ agglutinin: temperature-jump relaxation with fluorescence detection.
    Biochemistry. 1983; 22(20): 4797-804.

1981

  1. Smagowicz WJ, Castell JV, Clegg RM, Scheit KH.
    Properties of P3 esters of nucleoside triphosphates as substrates for RNA polymerase from Escherichia coli.
    Biochemistry. 1981; 20(19): 5538-46.
  2. Clegg RM, Loontiens FG, van Landschoot A, Jovin TM.
    Binding kinetics of methyl alpha-D-mannopyranoside to Concanavalin A: temperature-jump relaxation study with 4-methylumbelliferyl alpha-D-mannopyranoside as a fluorescence indicator ligand.
    Biochemistry. 1981; 20(16): 4687-92.

1980

  1. van Landschoot A, Loontiens FG, Clegg RM, Jovin TM.
    Binding kinetics of 4-methylumbelliferyl alpha-mannobioside to Concanavalin A by fluorescence stopped-flow measurements.
    Eur J Biochem. 1980; 103(2): 313-21.

1978

  1. Clegg RM Fulwyler MJ.
    A solution mixer with 10-μs resolution.
    Biophys J. 1978; 24(1): 57-58.
  2. van Landschoot A, Clegg RM, Loontiens FG.
    Kinetics of binding of 4-methylumbelliferyl alpha-mannobioside to Concanavalin A: stopped-flow measurements.
    Arch Int Physiol Biochim. 1978; 86(2): 464-6.

1977

  1. van Landschoot A, Loontiens FG, Clegg RM, Sharon N, de Bruyne CK.
    Binding of 4-methylumbelliferyl n-acetyl-chitooligosaccharides to wheat-germ agglutinin. A reinvestigation of equilibrium studies.
    Eur J Biochem. 1977; 79(1): 275-83.
  2. Loontiens FG, Clegg RM, van Landschoot A, Jovin TM.
    Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to tetrameric Concanavalin A Fluorescence temperature-jump relaxation study.
    Eur J Biochem. 1977; 78(2): 465-9.
  3. van Landschoot A, Clegg RM, Loontiens FG, Jovin TM.
    Binding of the 4 methylumbelliferyl glycosides of alpha-mannobiose and mannotriose to tetrameric Concanavalin A: equilibrium and fast kinetic studies using fluorescence quenching.
    Arch Int Physiol Biochim. 1977; 85(1): 203-4.
  4. Loontiens FG, Clegg RM, Jovin TM.
    Binding of 4-methylumbelliferyl alpha-d-mannopyranoside to dimeric and tetrameric Concanavalin A: equilibria and kinetic study using temperature-jump relaxation of fluorescence quenching.
    Arch Int Physiol Biochim. 1977; 85(1): 184-6.
  5. Clegg RM, Loontiens FG, Jovin TM.
    Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to dimeric Concanavalin A: fluorescence temperature-jump relaxation study.
    Biochemistry. 1977; 16(2): 167-75.
  6. Loontiens FG, Clegg RM, Jovin TM.
    Binding of 4-methylumbelliferyl alpha-D-mannopyranoside to tetrameric and unmodified or derivatized dimeric Concanavalin A: equilibrium studies.
    Biochemistry. 1977; 16(2): 159-66.

1976

  1. Clegg RM Maxfield BW.
    Chemical kinetic studies by a new small pressure perturbation method.
    Rev Sci Instrum. 1976; 47(11): 1383-1393.

1975

  1. Clegg RM.
    Relaxation kinetics applying repetitive pressure perturbations.
    PhD in Chemistry, Cornell University, 1975.
    Advisor: Elliot L Elson

1968

  1. Hammaker RM, Clegg RM, Patterson LK, Rider PE, Rock SL.
    Hydrogen-bonded dimers and the 2.86-μ band in alcohols.
    J Phys Chem. 1968; 72(5): 1837-1839.

1967

  1. Hammaker RM Clegg RM.
    The effect of halide salts on the NMR spectrum of methanol.
    J Mol Spectroscopy. 1967; 22(1-4): 109-112.