Laboratory for Fluorescence Dynamics

A NIH research resource center for biomedical fluorescence spectroscopy at the University of California, Irvine

Service

LFD is supported by

National Center for Research Resources

University of California, Irvine


Note

Inquiries about the availability of resources should be directed to the LFD User Coordinator.

Page Contents

Resources and Facilities

As of October 2006.

Microscopy

The LFD maintains nine microscope setups, many of which can be used for multiple types of experiments such as FCS, FLIM, RICS, N&B and particle tracking. All setups are using two-photon excitation and most are controlled by SimFCS software developed in the LFD. Refer to the description of microscope setups for technical details.

M1

M2

M3

M4

M5

TIRF

Users of this instrument are required to provide their own external USB hard drive for permanent data storage. One day of measurements typically generates 10-50 GB of uncompressible video data.

Olympus FV1000

Zeiss

ISS Alba

Spectroscopy

UV/visible Spectrophotometer

  • Perkin-Elmer Lambda 40

Steady-State Fluorescence

  • PC1
  • PC2
  • PC3

Time-Resolved Fluorescence

  • L1
  • L2: A time-domain setup based on a white light Laser and 4 Becker & Hickl SPC cards.
  • L3

Biochemistry Resources

  • Tissue culture room
  • Cold room
  • Cryofreezer ( -80°C)
  • Chromatography area
  • FPLC (Pharmacia)
  • Electrophoresis (Pharmacia)
  • Centrifuges (Beckman GPC & L8–70)
  • pH meter
  • pH-stat
  • Analytical balance
  • PhastSystem (Pharmacia)
  • Lipid extruder (Lipex)
  • Sonicator
  • Wet chemistry benches
  • Four fume hoods

Computing Resources

The LFD maintains more than 50 computers and printers, a computer lab, dozens of networking services, and hundreds of software packages. The computing facilities are regularly used by more than 25 people. The computer lab, data acquisition computers, servers, and printers are networked via a 1 Gbps Ethernet and are organized in a Windows Active Directory. Detailed documentation is available at the LFD Internal Computing Web site.

Users of the LFD facilities will be provided with a user account and network connections for personal computers. The LFD does not provide any software for private computers.

Computer Lab

The computer lab provides facilities for data storage and backup, data analysis and visualization, multimedia creativity, office productivity, and software development. The facilities are available to all members and guests of the LFD.

Hardware

  • Nine desktop computers operating under Windows XP Pro.
  • Two TB of networked data storage space with automated backup.
  • Black and white Laser printer and color InkJet printer.
  • Backup to LTO tape, CD and DVD media.
  • Audio, image, and video acquisition and processing.

Commercial Software

  • Globals for Images and Spectroscopy
  • Microsoft Office Pro 2003
  • Microsoft Visual Studio 2005
  • Adobe Acrobat 7
  • Adobe Creative Studio 2
  • Macromedia Studio 8
  • Thomson Endnote 9
  • IDM UltraEdit 10
  • Mercury Amira 4.1
  • Microcal Origin 7.5
  • Wavemetrics Igor Pro 5
  • MathWorks Matlab 7.5
  • Wolfram Mathematica 6.0
  • Nero Burning ROM 7
  • Camtasia Studio 2.1
  • ACD Canvas X
  • Visual Numerics PVWave 6.2
  • Fortner Noesys 2.4

Free or Open Source Software

  • OpenOffice
  • ImageJ, MIPAV, MeVisLab, VisBio, Slicer, MayaVi, ParaView
  • R, GnuPlot, Scilab
  • VMD, PyMol, RasMol
  • GIMP, Paint.NET, IrfanView, Blender, POV-Ray, OpenDX
  • VirtualDub, Audacity, VLC
  • Emacs, VIM, jEdit
  • Eclipse, Java SDK, MinGW
  • Python (inc wxPython, Numpy, Scipy, matplotlib, IPython)
  • MikTeX, WinEdit, LyX
  • Cygwin
  • many more...