Internal
Microscope Setups
As of May 2008
The Laboratory for Fluorescence Dynamics (LFD) maintains nine microscope setups, many of which can be used for multiple types of experiments such as FCS, FLIM, RICS, GP, N&B and particle tracking.
| M1 | M2 | M3 | M4 | M5 | |
|---|---|---|---|---|---|
| Type | Two-photon scanning FCS | Two-photon scanning FCS | Two-photon scanning FCS | Two-photon scanning FCS | Two-photon scanning FCS |
| Model | Custom | Custom | Custom | Custom | Custom |
| Microscope | Zeiss Axiovert 35 | Zeiss Axiovert 135TV | Zeiss Axiovert S100TV | Nikon Eclipse TE300 | Olympus IX70 |
| Ti:Sapphire LASER | Coherent Mira 900 | Spectra-Physics Mai Tai HP with integrated Millennia |
Spectra-Physics Tsunami 3941D-M11BB | Coherent Chameleon Ultra with integrated Verdi | |
| Pump LASER | Coherent Verdi-V5 | Spectra-Physics Millennia VsJs | |||
| Additional LASER | Coherent HeNe (1 mW) at 543.5 nm | ||||
| XY Scanner | Cambridge Technology Mirror scanner 6350 with controller series 603X servo system 6033 FM | P-730.20 with controller E-500.00 (1x E-509.C2A + 2x E-505.00) |
Cambridge Technology Mirror scanner 6350 with controller series 603X servo system 60335 FM | MCL xyz-piezo stage Nano-PDQ MCLS 01338 with NanoDrive85 controller | Cambridge Technology Mirror scanner 6350 with controller |
| Z Scanner | PI z-piezo objective drive P-721.10 with controller E-662 LVPZT-Amplifer/Servo | PI z-piezo objective drive P-721.LLQ PIFOC with E-509.X1+ E-505.00 controller | |||
| Detectors | Hamamatsu HC120-08 3963 PMTs |
Perkin Elmer APD Photon counting module SPCM-AQR-13-FC or SPCM-AQR-15 |
Hamamatsu H7422P-40 PMT |
Hamamatsu H7422P-40 PMT | Hamamatsu H7422P-40 PMT |
| Signal Amplifiers | MIT F-100T Pre-Amplifier | Becker & Hickel ACA-4-35-N 35 dB; 1.8 GHz | Becker & Hickel ACA-4-35-N 35 dB; 1.8 GHz | Becker & Hickel ACA-4-35-N 35 dB; 1.8 GHz | |
| Discriminators | Phillips Scientific CF Timing Discriminator 6915 | Phillips Scientific CF Timing Discriminator 6915 | Phillips Scientific CF Timing Discriminator 6915 | ||
| Cameras | Pixelink PL-A742 | Princeton Instruments, Roper Scientific 7386-0001 with controller 7513-0002 Pixelink PL-A641 |
Roper Scientific Photometrics Cascade 128+ Pixelink PL-A641 |
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| Interface Devices | ISS 3-Axis DAC v1.0 PCI ISS FCS v1.x PCI |
ISS 3-Axis DAC v1.0 PCI ISS FCS v1.x PCI |
ISS 3-Axis DAC v1.0 PCI ISS FCS v1.x PCI Becker & Hickel SPC-830 FlimBox USB |
ISS 3-Axis DAC v1.0 PCI ISS FCS v1.x PCI FlimBox USB |
ISS 3-Axis DAC v1.0 PCI ISS FCS v1.x PCI FlimBox USB |
| Computer | ASUS P4P800-E, Pentium IV 2.6 GHz, 2 GB RAM, 250 GB disk, 1 Gbps LAN | ASUS P4P800-E, Pentium IV 2.6 GHz, 2 GB RAM, 250 GB disk, 1 Gbps LAN | ASUS P4P800-E, Pentium IV 2.8 GHz, 2 GB RAM, 250 GB disk, 1 Gbps LAN | ASUS P4P800-E-Dlx, Pentium IV 2.6 GHz, 1 GB RAM, 250 GB disk, 1 Gbps LAN | ASUS P4P800, Pentium IV 2.8 GHz, 2 GB RAM, 250 GB disk, 1 Gbps LAN |
Software |
SimFCS | SimFCS | SimFCS | SimFCS | SimFCS |
Extras |
ISS Alba box 95305 | Motorized xyz-stage ASI Multi-Scan4 |
ISS Alba box 95305 |
Motorized xyz-stage ASI MS-4LS Harvard Apparatus Cell temp control PDMI-2 open perfusion micro-incubator |
|
| TIRF | OLYMPUS | ZEISS | ALBA | |
|---|---|---|---|---|
| Type | One-photon confocal | One-photon confocal | One-photon confocal | One or two-photon |
| Model | Custom | Olympus FluoView FV1000 | Zeiss LSM 510 META Confocor 3 | ISS ALBA FLIM |
| Microscope | Olympus IX81 | Olympus IX81 |
Zeiss Axiovert 200M |
Nikon Eclipse TE2000-U |
| LASERs | WorldStarTech TECBL diode (30 mW): 405 nm Melles Griot Argon Ion (150 mW): 457.9, 488, 514.5 nm HeNe (2 mW): 543.5 nm HeNe (10 mW): 594.1 nm |
Melles Griot Argon Ion (150 mW): 457.9, 488.0, 514.5 nm HeNe (5 mW): 543.5 nm HeNe (30 mW): 632.8 nm |
Argon Ion (150 mW): 457.9, 476.5, 488, 514.5 nm DPSS 561-10 (15 mW): 561 nmHeNe (5 mW): 633 nm |
Spectra-Physics Millennia/Tsunami |
| XY Scanner | A402 Scanning mirrors module with A401 scanning mirrors driver | |||
| Z Scanner | Piezo z-stage with A260 piezo stage controller z-axis | |||
| Detectors | Perkin Elmer SPCM-AQR-15 APD exchangable with Hamamatsu H7422P-40 PMT |
|||
| Cameras | Roper Scientific Photometrics Cascade 512B and 128+ EMCCD | |||
| Interface Devices | ISS 3-Axis DAC v1.0 PCI ISS FCS v1.x PCI ISS A320 FastFLIM (FlimBox) USB |
|||
| Computer | Intel S875WP1-E, Pentium IV 3.0 GHz CPU, 2 GB RAM, 1 Gbps LAN, 250 GB + 1.3 TB RAID0 disks | Dell Precision 390, Core Duo 1.8 GHz CPU, 2 GB RAM, 1Gbps LAN, 160 GB disk | ||
| Software | ISS Vista, SimFCS | |||
Extras |
Magnification changer (1x, 2x, 4x) Olympus TIRFM illuminator Olympus UPlanApoN 60x/1.45 Oil TIRFM Olympus Apo 100x/1.65 Oil HR [0.15 sapp] |
A211 Synchronization modulePower tower |
Schematic of a two-photon scanning FCS microscope:
