Laboratory for Fluorescence Dynamics

A NIH research resource center for biomedical fluorescence spectroscopy at the University of California, Irvine

General Information

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LFD is supported by

National Center for Research Resources

University of California, Irvine


Picture Tour

The Laboratory for Fluorescence Dynamics (LFD) occupies a ~8000 sq. ft. facility at the Biomedical Engineering Department in the interdisciplinary Natural Sciences 2 building of the University of California, Irvine. It maintains a state-of-the-art laboratory for fluorescence measurements with resources for spectroscopy, microscopy, biochemistry, cell culture, and data analysis.

Welcome

The Natural Sciences 2 building. The LFD is located in the 3rd floor:

Natural sciences 2 building

Laboratory members, May 2007:

People

LFD offices:

LFD offices

The LFD is a biomedical technology research center sponsored by the NIH:

NCRR center

The Biomedical Engineering Department laboratory tract:

BME laboratories

Computer Laboratory

Data analysis workstations, file servers, and printers:

Computer lab

Computer lab

Imaging and backup workstation:

Image workstation

Biochemistry Laboratory

Laboratory benches:

Biochemistry lab

Biochemistry lab

Chemical fume hoods:

Hoods

pH Meters and scales:

pH meters and scales

Ultracentrifuge and -80°C freezer:

Centrifuge and freezer

Cell and tissue culture laboratory:

Cell culture lab

Spectroscopy Instrumentation

Perkin Elmer UV-Vis absorption spectrometer:

UV-Vis spectrometer

Horiba Jobin-Yvon FluoroMax-4 fluorimeter:

FluoroMax-4

ISS PC1 fluorimeter:

Fluorimeter

A custom multifrequency cross-correlation phase and modulation fluorometer:

L1

A custom time-domain fluorometer with Fianium Supercontinuum laser and Becker & Hickl TCSPC card:

L2

Microscopy Instrumentation

Microscope 1 – Fluorescence correlation and generalized polarization imaging:

Microscope 1

Microscope 3 – Fluorescence lifetime imaging in the frequency and time domain:

Microscope 3

Microscope 4 with ISS Alba box– Fluorescence correlation spectroscopy and lifetime imaging:

Microscope 4

Microscope 5 – Particle tracking in three dimensions:

Microscope 5

Olympus TIRF microscope with Photometrics Cascade cameras:

TIRF microscope

Zeiss LSM 510 META ConfoCor 3 microscope:

Zeiss microscope

Olympus FV1000 confocal laser scanning microscope:

Olympus microscope

Other Facilities

Electronics laboratory:

Electronics laboratory