Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

6th Weber Symposium (2005)

The 6th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine was held in Kauai, Hawaii, on July 22-28, 2005.

The "8th International Carl Zeiss-sponsored Workshop on Fluorescence Correlation Spectroscopy (FCS) and related Methods" was held on Saturday, July 23, 2005, as part of the Weber Symposium.

This symposium will honor the fundamental and far-reaching contributions of Professor Gregorio Weber. It will provide a current overview of modern fluorescence methodologies and applications in the biological and medical sciences, and is the 6th in a series. Previous symposia were held in Italy in 1986 and 1991, and in Hawaii in 1995, 1999, and 2002.

As in the past, speakers will describe state-of-the-art and emerging technologies used in their research programs. Topics to be addressed will include multiphoton fluorescence microscopy, confocal microscopy, time-resolved fluorescence instrumentation, single molecule studies, fluctuation correlation spectroscopy, fluorescence imaging spectroscopy, fluorescence resonance energy transfer, macromolecular (nucleic acids and proteins) interactions, membrane dynamics, and quantitative cellular dynamics. 

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

Schedule

Friday, July 22

  • 3:00 – 6:00 PM: Registration and Poster and Exhibit Setup
  • 7:30 PM: Welcome by Enrico Gratton
  • 7:45 PM: Opening lecture by Dave Jameson.
    A nano-history of fluorescence (with some comments about the contributions of Gregorio Weber to modern fluorescence spectroscopy).
  • 8:30 PM: Cocktail Reception

Saturday, July 23

8th International Carl Zeiss-sponsored Workshop on Fluorescence Correlation Spectroscopy (FCS) and related Methods

  • 7:30 – 9:00 AM: Continental Breakfast, Posters, and Exhibits
  • 9:00 AM: Enrico Gratton
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL.
    Spatio-temporal correlations for the study of fluctuations in the cell interior.
  • 9:30 AM: Elliott Elson
    Department of Biochemistry and Molecular Biophysics; Washington University School of Medicine.
    Dependence of the clustering of EGF receptors on membrane cholesterol.
  • 10:00 AM: Cècile Fradin
    Department of Physics and Astronomy, McMaster University, Hamilton, ON, M5R2Y9, Canada.
    Mobility of proteins involved in nuclear transport.
  • 10:30 AM: Beverage Break
  • 11:15 AM: Joachim Mueller
    School of Physics and Astronomy, University of Minnesota, Twin Cities, 116 Church St. S.E., Minneapolis.
    Quantitative resolution of protein homo- and hetero-interactions in cells.
  • 11:45 AM: Saveez Saffarian*
    Department of Cell Biology, Harvard Medical School, Boston.
    Clathrin coat formation in vivo, studied one molecule at a time.
  • 12:15 PM: Nancy Thompson
    Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC.
    Protein dynamics near substrate-supported membrane surfaces: measurement by total internal reflection with fluorescence correlation spectroscopy.
  • 12:45 – 2:15 PM: Free for Lunch
  • 2:15 PM: Petra Schwille
    BIOTEC, Dresden, Germany.
    Perspectives for confocal single molecule analysis in cells and membrane systems.
  • 2:45 PM: Michelle Digman
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL.
    Diversity of structure and dynamics of focal adhesions revealed by orbital scanning FCS, line scanning FCS and raster image correlation spectroscopy.
  • 3:15 PM: Theodore Hazlett (invited talk)
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL.
    Rotational dynamics from the autocorrelation of the sample polarization time series.
  • 3:30 PM Zeiss Sponsor Talk
  • 3:45-5:30 PM: Posters/Exhibits
  • 6:00 – 8:00 PM: Cocktail Reception Sponsored by Zeiss

Sunday, July 24

  • 8:00 – 9:30 AM: Continental Breakfast, Posters-Exhibits
  • 9:30 AM: Luis Bagatolli
    University of Southern Denmark, Department of Biochemistry and Molecular Biology, Odense, Denmark.
    Coexistence of two fluid phases in native pulmonary surfactant membranes: cholesterol rules.
  • 10:00 AM: Susana Sanchez
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL.
    Laurdan GP, RHDL and cholesterol removal.
  • 10:30 AM: Miguel Castanho (invited talk)
    Molecular Biophysics Laboratory, Centro de Química e Bioquímica, Faculdade de Ciências da Universidade de Lisboa, Ed. C8, Campo Grande, 1749-016 Lisboa, Portugal.
    Translocation of b-galactosidase mediated by PEP-1 into large unilamellar vesicles and human hela cells is driven by membrane electrostatic potential.
  • 10:45 AM: Beverage Break
  • 11:30 AM: Globals
  • 11:45 AM: ISS
  • 12:00 AM: HORIBA Jobin-Yvon
  • 12:15 AM: Varian
  • 12:30 AM: Becker&Hickl
  • 12:45 AM: Evotec
  • 1:00 -2:30 PM: Free Time
  • 2:30 PM: George Barisas
    Department of Chemistry, Colorado State University, Fort Collins, CO.
    Continuous fluorescence depletion anisotropy measurement of protein rotation.
  • 3:00 PM: Robert Clegg
    Laboratory for Fluorescence Dynamics, Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL.
    Measurements of internal distance changes of the 30S ribosome using FRET with multiple donor-acceptor pairs: quantitative spectroscopic methods.
  • 3:30 PM: Sergey Tetin (invited talk)
    Core R&D Biotechnology, Abbott Diagnostics Div., Abbott Park, IL.
    High resolution antigenic epitope mapping using fluorescence correlation spectroscopy.
  • 3:45 – 4:15 PM: Beverage Break
  • 4:15 PM: Amitabha Chattopadhyay
    Centre for Cellular and Molecular Biology, Hyderabad 500 007, India.
    Monitoring receptor organization and dynamics using enhanced yellow fluorescent protein-tagged serotonin-1a receptor.
  • 4:45 PM: Andrew Clayton
    Ludwig Inst. for Cancer Research, Melbourne Tumour Biology Br.,Royal Melbourne Hospital, Victoria, Australia.
    Ligand induced dimer-tetramer transition during the activation of the cell surface epidermal growth factor receptor.
  • 5:15 PM: Thomas Jovin (invited talk)
    Department of Molecular Biology, Max Planck Inst. for Biophysical Chemistry, am Fassberg 11, Göttingen, Germany.
    Quantum dot (single particle) tracking of egf receptor undergoing retrograde transport on fiolopodia: single molecule sensing by cells.
  • 5:30 PM: Rob Learmonth (invited talk)
    Centre for Rural and Environmental Biotechnology, Department of Biological and Physical Sciences, U.of Southern Queensland, Toowoomba, Queensland, Australia.
    Fluorescence-based screening of microbial status during bioprocesses.
  • 5:45-7:30 PM: Free Time
  • 7:30 – 8:15 PM: Antonie Visser (2005 Weber Lecturer)
    MicroSpectroscopy Centre, Laboratories of Biochemistry and Biophysics, Wageningen University, Wageningen, The Netherlands.
    FRET adventures with flavoproteins and visible fluorescent proteins.
  • 8:15 PM Weber Prize Winners Introduction
  • 8:20 PM Harold Kim*
    Department of Physics, Stanford University, 382 Via Pueblo Mall, Stanford, CA.
    Monitoring motions of tRNAs in the ribosome using single-molecule FRET.
  • 8:50 PM Ahmet Yildiz*
    Department of Cellular and Molecular Pharmacology, University of California, SF, CA.
    Fluorescence imaging with one naometer accuracy: application to molecular motors.

Monday, July 25

  • Free all day

Tuesday, July 26

  • 8:00 – 9:30 AM Continental Breakfast Posters-Exhibits
  • 9:30 AM: Gerard Marriot
    Department of Physiology, University of Wisconsin, Madison, WI.
    Optical switching of dipolar interactions on proteins.
  • 10:00 AM: David Jameson
    University of Hawaii, Honolulu, HI.
    Protein interactions of the large GTPase Dynamin
  • 10:30 AM: Jaroslava Miksovska (invited talk)
    Deparmtent of Chemistry, Marshall University, Huntington, WV.
    Monitoring of proton release in water pools associated with reverse micelles.
  • 10:45 AM: Beverage Break
  • 11:30 AM: Pierre Moens
    School of Biological, Biomedical & Molecular Sciences, The University of New England, Armidale, NSW 2351 Australia.
    Oligomeric state and mode of self-association of thermotoga maritima ribosomal stalk protein l12 in solution.
  • 12:00 AM: Octavio Monasterio
    Departamento de Biologia, Facultad de Ciencias, Universidad de Chile, Casilla 653, Santiago, Chile.
    Fluorescence spectroscopy applied to tubulin and FTSZ unfolding/folding.
  • 12:30 PM: Moshe Levi
    University of Colorado Health Sciences Center, Depts of Medicine, Physiology and Biophysics, Denver, CO.
    Partitioning of napi cotransporter in cholesterol, sphingomyelin and glycosphingolipid enriched membrane microdomains modulates napi protein diffusion, clustering and activity.
  • 1:00 – 2:30 PM: Free Time
  • 2:30 PM: Kazuhiro Oiwa
    Kansai Advanced Research Center, Kobe, Japan.
    Molecular mechanisms of AAA-proteins, FTSH and dynein studied with total internal reflection fluorescence (TIRF) microscopy.
  • 3:00 PM: Taekjip Ha
    Department of Physics, University of Illinois, Urbana-Champaign, Urbana, IL.
    Porous nanocontainers for single molecule analysis.
  • 3:30 PM: Parkson Chong (invited talk)
    Department of Biochemistry, Temple University School of Medicine, Philadelphia, PA.
    Effect of sterol superlattice on free-radical induced sterol oxidation and on antioxidant protection in lipid membranes.
  • 3:45 – 5:30 PM: Posters/Exhibits

Wednesday, July 27

  • 8:00 – 9:30 AM: Continental Breakfast Posters-Exhibits
  • 9:30 AM: Gregory Reinhart
    Department of Biochemistry and Biophysics, Texas A&M, College Station, TX.
    Use of hybrid proteins to study oligomeric properties.
  • 10:00 AM: Paul Wiseman
    Department of Physics, McGill University, 3600 University St., Montreal, QC, Canada.
    Cellular cartography using spatio-temporal image correlation spectroscopy.
  • 10:30 AM: James Herron (invited talk)
    Department of Pharmaceutics and Pharmaceutical Chemistry, University of Utah, Salt Lake City, UT.
    Real-time detection of single base extension reactions using planar waveguide fluorescence sensors.
  • 10:45 AM: Beverage Break
  • 11:30 AM: Paul Selvin
    Department of Physics, University of Illinois at Urbana-Champaign, Urbana, IL.
    Fluorescence imaging with one nanometer accuracy (FIONA): application to molecular motors.
  • 12:00 AM: David Piston
    Department of Molecular Physiology and Biophysics, Vanderbilt University, Nashville, TN.
    Imaging the molecular pathways of glucose-stimulated insulin secretion with multimodal laser scanning microscopy.
  • 12:30 PM: David Lloyd (invited talk)
    University of Tennessee Space Institute, Tullahoma, TN.
    Accounting for saturation and triplet effects in FCS measurements.
  • 12:45 PM Closing Comments
  • 6:00 – 9:00 PM: Special Luau for Symposium Sponsored by Globals
    Each registered participant is admitted free of charge to the special luau. $60 will be required for each unregistered guest of participants who choose to attend this special luau. 

(*) Weber Prize Winners, Sponsored by UIUC Weber Prize Fund

General Information

The venue for the meeting is the same as that in 2002: the Kauai Marriott Resort and Beach Club, located on Kalapaki Beach, Lihue, Hawaii, on the Island of Kauai. The address is Kalapaki Beach, 3610 Rice Street, Lihue-Kauai, Hawaii 96766.

We have negotiated a room rate of $195 plus 11.416% tax per night, which is applicable for the dates of the symposium, plus 3 days before and 3 days after the symposium. This rate is significantly lower than the standard room rate at this beautiful resort. Please make your own arrangements with the Kauai Marriott Resort at 1-800-220-2925, or refer to the Marriott's web site for information when calling from outside the USA. Please identify yourself as being with the "6th Weber Meeting" in order to receive the special symposium rate. All participants, including invited speakers, must make reservations with the hotel. If you have difficulty reserving rooms at the special rate, please ask the reservationist to check with events management.

Speakers will describe state-of-the-art and emerging technologies used in their research programs. As in the past symposia, invited speakers will be given 25-minutes for their presentation with 5-minutes for questions and answers. Topics include multiphoton fluorescence microscopy, confocal microscopy, time-resolved fluorescence instrumentation, single molecule studies, fluctuation correlation spectroscopy, fluorescence imaging spectroscopy, fluorescence resonance energy transfer, macromolecular interactions, membrane dynamics, and quantitative cellular dynamics.

Sponsors ($4000 contribution or more) will be invited to give a 15-minute oral presentation about their company's products, in addition to the other sponsor benefits. These presentations will be during the regular day program. Exhibitors at the $2500 level will not give an oral presentation.

Exhibits will be on display throughout the symposium, in an area immediately next to the meeting room, in the same room as the coffee breaks and poster sessions. Sponsors ($4000 level and above), as well as exhibitors ($2500 level), may exhibit.

Posters will also be on display throughout the symposium. Times will be announced for the poster presentation sessions.

Ample time will be provided for all to visit the exhibit booths, posters, and to take advantage of the luxurious island amenities (see schedule).

The deadline for registration is June 1, 2005. Posters are not required for attendance.

Further information can be obtained from any of the organizers: David M. Jameson, or Enrico Gratton, or the Conference Secretary.

Registration

Payment

Registration may be paid in the following ways:

  • International check, payable to "University of Illinois," mailed to the Conference Secretary.
  • Credit card. The credit card server is a separate, secure server, operated by the University of Illinois Accounting Division.
  • On-site via cash. Checks or credit card payments cannot be accepted on-site.

Regular Participants

  • $400 Professional
  • $200 Student

Sponsors

  • $4000 Exhibit space (10x6 foot*), suitable for a stand-alone display. This includes electricity (110V, 60Hz), table, two chairs, company listing in abstract book, full-page, color advertisement in abstract book (exhibitor must supply copy) and admission to full scientific program for two persons connected with the exhibit. Sponsors will be allowed 15-minutes each to make a presentation about their company's product, will be indicated as a "Sponsor" in abstract book and their name will be prominently displayed at symposium, plus a special mention by the organizers. Includes continental breakfasts and beverage breaks for the two named representatives.

Exhibitors

  • $2500 Exhibit space (10x6 foot*), suitable for a stand-alone display. This includes electricity (110V, 60Hz), table, two chairs, company listing in abstract book, full-page, color advertisement in abstract book (exhibitor must supply copy) and admission to full scientific program for one person connected with the exhibit, also includes continental breakfasts and beverage breaks.
  • $1000 Table top display space. Approximate size is half of a 60x30-inch*conference table. It is not suitable for an equipment display. Except for lighting, electricity is not provided.

Advertising Only

  • $500 Full page (7.5x10 inch*) color advertisement in abstract book.
  • $300 Full page (7.5x10 inch*) black/white advertisement in abstract book.

Exhibits will be on display throughout the symposium. The exhibit/poster area is adjacent to the main lecture hall. Prior to the meeting, exhibition materials and instrumentation may be shipped directly to the Marriott, where they will be held in a secure area.

(*) Sizes are approximate.

Posters Information

All participants who wish to hang a poster and all speakers must submit an abstract to the Conference Secretary. More than one poster may be submitted if approved by the Committee. Abstracts and posters must be scientific in nature; posters containing advertising are not allowed. Abstracts should be no larger than to fit in a space approximately 6.5"x6.5" (width x height) , single-spaced in a 10-point font. Please submit your abstract in one of the following ways: on-line registration, on magnetic disk (in almost any format, Microsoft Word preferred), accompanied by a hard copy of the text, hand typed, or submitted via electronic mail. Abstracts will be typeset for publication in the program.

Deadline for submitting abstracts for inclusion in abstract book is June 1, 2005.

Size of poster area: Individual, free-standing poster boards will be used this year. The area allotted for each poster will be 4x4 feet. If your poster is in one large piece, it may extend below the 4-foot area, but cannot be wider than 4 feet.

Sponsor and Exhibitor Information

Purpose

The purpose of the exhibits is to further the education of the registrants by providing an area for exhibitors to present information, products, or services relevant to the theme of the symposium. The exhibits must be of an educational character and emphasize instrumentation, products, or services directly relevant to the interests of the symposium participants, i.e., teaching, research, or directly convey scientific research findings in those areas of science represented by the symposium theme.

Exhibits will be on display throughout the symposium. The exhibit area is directly adjacent to the main lecture hall. (Participants will pass through exhibitions to enter the lecture hall.)

Shipping Equipment and Materials

Ship equipment after July 8th to the Kauai Marriott. Specify on the shipping label that the material is for the 6th Weber Symposium and should be shipped to:

Kauai Marriott Resort
6th Weber Symposium
Attn: Silvia Gama
Convention Services
3610 Rice St.
Lihue, HI 96766

Any questions can be directed to Silvia Gama at (808) 246-5071.

Space Assignment

Exhibit spaces are assigned by Exhibit Management. Space assignments will be made on a first-come, first-served basis. Whenever possible, space will be allotted according to the exhibitor's choice, but the final arrangement will be determined by Exhibit Management in such a way to produce the most advantageous grouping. The symposium and the hotel will have no liability if the location assigned is not as requested. Applications for space must be received by May 01, 2005 in order to be listed in the program. A brief description (approx. 50 words) of the products or services to be displayed in your exhibit area must also be submitted (see exhibit application). This information will be published in the program.

Location Relative to Other Exhibits

Exhibitors may use the exhibit application form to request their preference for location near other companies or their wish to not be adjacent to or opposite designated companies. These requests will be honored to the greatest extent possible in conjunction with assignment priorities and requests for specific locations on the floor.

General Conduct of Exhibitors

The following are prohibited:

  • Noisy electrical apparatus or other mechanics, flashing lights, music, sounds, or gestures that would interfere with other exhibitors or that would disrupt the symposium at anytime.
  • Removing or adjusting any lighting or electrical connection that is not connected specifically with your exhibit.
  • Entering, photographing, video taping, or examining another exhibitor's equipment without permission.
  • Sub-leasing of exhibit space.
  • Dismantling exhibits and leaving before official close of the symposium.

Distribution of Giveaways

Exhibitors are encouraged to provide giveaway items or other appropriate promotional materials. Typically, these items have been assembled in a package provided for each participant of the symposium at registration. Items for distribution should be sent after July 8th to:

Kauai Marriott Resort
6th Weber Symposium
Attn: Silvia Gama
Convention Services
3610 Rice Street
Lihue, HI 96766

Any questions can be directed to Silvia Gama at (808) 246-5071.

Registration of Exhibitors

Exhibitors may register the specified number of members of their organization for each single exhibit or table space reserved. Additional attendees must register and pay the required participant registration fee. It is the responsibility of the authorized individual signing the application for space to inform all company personnel of the regulations contained herein. Name tags will be distributed to registered exhibitors. Badges are not transferable. The badge of a departing representative may be turned in at the Registration Desk and a new badge will be issued to the exhibitor taking that person's place. Official meeting badges must be worn at all times while in the exhibit area. Persons without official meeting badges will not be permitted in the symposium or exhibit area.

Guests of Exhibitors

Only properly identified and authorized registrants will be permitted to enter the exhibit area. Badges will be made available to guests of exhibitors, but all guests must register at the Registration Desk. It is the responsibility of the exhibiting company to inform all guests of the regulations contained herein.

Cancellation or Reduction

Notification of an exhibitor cancellation must be made in writing. If space is cancelled or reduced between 50 and 30 days prior to the opening date of the meeting, 10% of the rental fee will be retained by the symposium. If space is cancelled between 30 and 15 days prior to the opening date of the meeting, 50% of the rental fee will be retained by the symposium. If the space is cancelled 15 days or less before the opening of the symposium, the entire rental fee will be retained by the symposium.

Enforcement of Regulations

Each exhibitor is granted nothing more than a terminable license to exhibit, subject to all rules herein and is under the general control of Exhibit Management and the Kaua'i Marriott. If the exhibitor fails to comply with any rule herein, the license may be terminated and the exhibit closed without notice. All interpretation of the rules and regulations indicated herein remain the discretion of Exhibit Management and their decision is final.

Advertising

All advertisements will be duplicated on a professional photocopy machine direcly from the copy submitted. The area of advertisement copy must be no larger than approximately 7.5x10 inches. Finished page size will be 8x11 inches. If advertiser wishes better resolution than a copier can provide, they may send 200 prepared copies of the advertisement directly to Conference Secretary. Copies must be on paper no heavier than 20-lbs. Copies for duplication may be sent as hard copies or electronically, as a Microsoft Word document, or as a gif, jpeg file. If in doubt about the suitablity of your submission, contact the Conference Secretary. The deadline for submitting advertising copy for duplication is June 1, 2005.

The placement of advertisements in the abstract book is alphabetical in logic. In addition to the 8x11 inch page of advertisement, there will be an alphabetical listing of advertisers in the table of contents section of the abstract book, as well as an alphabetical listing of advertisers displayed and acknowledged at the beginning of the symposium (in an over-head type projection). Also, a link to advertiser's Web site will be included in the listing of advertisers on our Web site.

Conference Secretary

Julia K. Wright, Staff Secretary
Department of Physics
University of Illinois at Urbana-Champaign
1110 W. Green Street
Urbana, IL 61801-3080
phone: +1 217-244-7186
fax: +1 217-244-7187
email: wrightj@uiuc.edu