Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

Carl Zeiss Inc. Logo

Globals Software

Calendars

Notes

10th Weber Symposium (2017)

The 10th International Weber Symposium on Innovative Fluorescence Methodologies in Biochemistry and Medicine was held on May 28 - June 2, 2017 at the Hotel Atlântico Búzios in Búzios, Brazil.

Agenda Calendar

Sunday, May 28, 2017

  • 15:00 – Registration, poster and exhibit setup.
  • 19:30Welcome. Jerson Silva, Andre Gomes, Andréa Cheble de Oliveira, David M Jameson, and Enrico Gratton.
  • 19:45Opening lecture. David M Jameson: The seminal contributions of Gregorio Weber to modern fluorescence spectroscopy and to protein chemistry.
  • 20:30 – Cocktail reception at swimming pool sponsored by Globals Software.

Monday, May 29, 2017

  • 07:30 – Breakfast at dining room, followed by posters and exhibits.
  • Chair: Michelle Digman
  • 09:00Lecture 2. Enrico Gratton: Correlation of molecular diffusion in live cells.
  • 09:30Lecture 3. Elliot Elson: Measurement of the kinetics of protein synthesis and degradation in non-equilibrium tteady states using "Light Jumps".
  • 10:00Lecture 4. Jerson Silva: Prion-like aggregation of proteins in cancer: the "Dr. Jekyll and Mr. Hyde" actions of p53.
  • 10:30 – Break, posters and exhibits.
  • Chair: Francesco Cardarelli
  • 11:15Lecture 5. Debora Foguel: The role of microglia and neutrophils in amyloidosis.
  • 11:45Lecture 6. Rachel Cinco-Hedde: Spatial characterization of bioenergetics and metabolism of primordial to preovulatory follicles in whole ex vivo murine ovary.
  • 12:15Lecture 7. Manuel Prieto: Advanced homo-FRET: exact analytical solutions for tetramers and their application for retrieving structural information of the KcsA channel.
  • 12:45 – Lunch at dining room.
  • Chair: David M Jameson
  • 14:00Lecture 8. Luis Bagatolli: Dynamical coupling between intracellular water relaxation and oscillating glycolysis in yeast cells.
  • 14:30Lecture 9. Andre Gomes: Cell metabolism, membrane organization and virus entry during Arboviruses infections.
  • 15:00Lecture 10. Amit Chattopadhyay: Membrane dipole potential: applications in membrane and cell biology.
  • 15:30Lecture 11. Chiara Stringari: Multicolor two-photon imaging of endogenous fluorophores in living tissues by wavelength mixing.
  • 16:00 – Break, posters and exhibits.

Tuesday, May 30, 2017

  • 07:30 – Breakfast at dining room, followed by posters and exhibits.
  • Chair: Luca Lanzanò
  • 09:00Lecture 12. Fernando Stefani: Nanometer resolution single molecule localization with minimal photon fluxes.
  • 09:30Lecture 13. Francisco Barrantes: Nanoscopy of nicotinic acetylcholine receptors: cholesterol effects on supramolecular topography and dynamics.
  • 10:00Lecture 14. Gerd Ulrich Nienhaus: Stimulated emission double depletion (STEDD) for background suppression in nanoscale imaging and fluorescence fluctuation spectroscopy.
  • 10:30 – Break, posters and exhibits.
  • Chair: Enrico Gratton
  • 11:15Lecture 15. Markus Sauer: Super-resolution fluorescence imaging by dSTORM: where next?.
  • 11:45Lecture 16. Joachim Mueller: Venturing into the nuclear envelope: lessons learned.
  • 12:15Lecture 17. Francesco Cardarelli: Probing the dynamic fingerprint of insulin secretory granules in living cells by spatiotemporal fluctuation spectroscopy.
  • 12:45 – Lunch at dining room.
  • Chair: Andre Gomes
  • 14:00Lecture 18. Gregory Reinhart: Reconciling Weber's protein-ligand ideas with contemporary data using fluorescence phasors.
  • 14:30Lecture 19. Leonel Malacrida: Water dipolar relaxation inside nucleus: taking advantage of ACDAN spectroscopic properties and spectral phasors.
  • 15:00Lecture 20. Susana Sanchez: Using Laurdan and phasor analysis to follow solubilization of rabbit erythrocytes membranes by myristyl sucrose ester.
  • 15:30Lecture 21. Soledad Celej: On the structure and membrane-binding properties of oligomeric species of the amyloidogenic protein α-synuclein.
  • 15:45Lecture 22. Sergi Padilla-Parra: Multi-faceted role of dynamin during HIV entry.
  • 16:00 – Selected posters, short presentations (to be selected).
  • 16:15 – Break, posters and exhibits.
  • Chair: Enrico Gratton
  • Weber International Thesis Prize Winner
  • 18:00Lecture 23. Rupsa Datta: Label-free fluorescence lifetime imaging microscopy (FLIM) to study metabolism and oxidative stress in biological systems.
  • Thesis Honorable Mention
  • 18:25Lecture 24. Zeno Lavagnino: Improving selective plane illumination microscopy towards enhanced spatial resolution and imaging depth performances.
  • 18:50Lecture 25. Victoria Neguembor: Investigating the chromatin fiber at the nanoscale level.

Wednesday, May 31, 2017

  • 07:30 – Breakfast at dining room, followed by posters and exhibits.
  • Chair: Jerson Silva
  • 09:00Lecture 26. Valeria Levi: Mapping the dynamics of transcription factors in living cells and whole organisms.
  • 09:30Lecture 27. Xavier Darzacq: Single molecule imaging of morphogen gradients during early fly development.
  • 10:00Lecture 28. Diane Lidke: Imaging early events in membrane receptor signaling.
  • 10:30 – Break, posters and exhibits.
  • Chair: Rajesh Ramachandran
  • 11:15Lecture 29. Maria Parajo: Spatiotemporal organization of biological membranes using nanophotonic tools.
  • 11:45Lecture 30. Per Niklas Hedde: Selective plane illumination microscopy in the conventional inverted microscope geometry.
  • 12:15Lecture 31. Keith Lidke: Single objective light-sheet microscopy for improved whole-cell 3D super-resolution.
  • 12:35 – Lunch at dining room.
  • Chair: Gregory Reinhart
  • 14:00Lecture 32. Marian Waterman: Wnt signaling links to metabolism in cancer and stem cells.
  • 14:30Lecture 33. Don Lamb: The fantastic voyage: 3D tracking of mitochondria in zebra fish embryos.
  • 15:00Lecture 34. Michelle Digman: Imaging techniques to measure spatio-temporal dynamics and metabolic alterations of p53 upon DNA damage.
  • 15:30Lecture 35. Mário Berberan-Santos: Phasors, spectral phasors andcomplementary approaches.
  • 16:00 – Break, posters and exhibits.

Thursday, June 1, 2017

  • 07:30 – Breakfast at dining room, followed by posters and exhibits.
  • Chair: Francisco Barrantes
  • 09:00Lecture 36. Rajesh Ramachandran: Cardiolipin-enriched microdomains sequester adaptors MiD49/51 and Mff to cooperatively regulate Drp1-mediated mitochondrial fission.
  • 09:30Lecture 37. Ibrahim Cisse: Capturing genome regulation with single molecule resolution in live mammalian cells.
  • 10:00Lecture 38. Luca Lanzanò: Phasor analysis of fluorescence dynamics in quantitative nanoscale optical microscopy.
  • 10:30 – Break, posters and exhibits.
  • Chair: Andréa Cheble de Oliveira
  • 11:00Lecture 39. Jose Luiz DeSouza Lopes: Exploiting the thermal stability and organic solvent tolerance of esterases.
  • 11:30Lecture 40. Carlos Lenz: Fluorescence correlation spectroscopy in crude oils and Quantum Dots.
  • Sponsor Talks
  • 12:00Lecture 41. Christian Hellriegel (Carl Zeiss Microscopy): Controls and calibrations: details that matter.
  • 12:15Lecture 42. Enrico Gratton (Globals Software): Fast spatial-correlation methods for camera based images and fast laser scanning microscopes.
  • 12:35Closing remarks and acknowledgments. Jerson Silva and Enrico Gratton.
  • 12:45 – Lunch at dining room.
  • 18:00 – Symposium Banquet.

Friday, June 2, 2017

  • Free day

Abstracts by corresponding author

Attendees in alphabetical order

Organizing Committee

  • David M. Jameson
    Professor of Cell and Molecular Biology, John A Burns School of Medicine, University of Hawaii at Manoa.
  • Enrico Gratton
    Principal Investigator of the LFD. Professor of Biomedical Engineering, Physics, and College of Medicine, University of California, Irvine.
  • Jerson L. Silva
    Federal University of Rio de Janeiro, Brazil.
  • Andre Gomes
    Federal University of Rio de Janeiro, Brazil.
  • Andréa Cheble de Oliveira
    Federal University of Rio de Janeiro, Brazil.