Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Flyer

LFD Workshop Flyer

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

Center for Complex Biological Systems

CMC Logo

Globals Software

ISS Inc.

Leica Microsystems

Mathematical, Computational and Systems Biology

Carl Zeiss Inc.

Photos

Group Photo

Computer Training

Abbreviations

  • FCS: Fluorescence Correlation Spectroscopy
  • FLIM: Fluorescence Lifetime Imaging
  • FRET: Förster Resonance Energy Transfer
  • ICS: Image Correlation Spectroscopy
  • kICS: k-space Image Correlation Spectroscopy
  • N&B: Number and Brightness
  • PCH: Photon Counting Histogram
  • PSF: Point Spread Function
  • PT: Particle Tracking
  • RICS: Raster Image Correlation Spectroscopy
  • STICS: Spation-Temporal Image Correlation Spectroscopy
  • more...

Notes

  • Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
  • Adobe Flash Player 9 is required to view the recordings.

2nd LFD Workshop (2007)

The "2nd LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 22–26, 2007, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.

Lecture Recordings

Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.

Agenda and Downloads

Monday, October 22, 2007

Tuesday, October 23, 2007

  • Breakfast
  • 09:00-09:45 – Lecture 4. Enrico Gratton: Introduction to ICS PDF
  • 09:45-10:30 – Lecture 5. Paul Wiseman: STICS and kICS PDF
  • Break
  • 11:00-11:45 – Lecture 6. Michelle Digman: The RICS method PDF
  • 11:45-12:15 – Application 2. Michelle Digman: Applications of RICS
  • 12:15-12:45 – Fellowship talks
    • Pabak Sarkar: Photophysical study of Cerulean and Venus fluorescent proteins
    • Nick Watson: Intracellular dynamics of proteins required for the resolution of DNA replication forks blocked by genotoxic carcinogens
    • Leor Weinberger: Gene expression noise correlation spectroscopy to quantify transcriptional processes in live cells
  • Lunch
  • 13:30-15:00 – Computer based training on RICS. Download Computing tutorials PDF and data files ZIP
  • Break
  • 15:30-17:20 – Computer based training on RICS continued.
  • 17:20-18:00 – Special lecture 1. Robert M Clegg: The history of FRET PDF
  • 18:00-20:00 – Social event.

Wednesday, October 24, 2007

Thursday, October 25, 2007

Friday, October 26, 2007

  • Breakfast
  • 09:00-10:30 – Laboratory training. Download Laboratory tutorials PDF
  • Break
  • 11:00-12:45 – Laboratory training continued.
  • Lunch
  • 13:30 – Closure

Lecturers

  1. Asmahan Abu-Arish
    Department of Physics and Astronomy, McMasters University, Canada.
  2. Valeria R Caiolfa
    Fondazione Centro San Raffaele, Scientific Institute, Milan, Italy.
  3. Robert M Clegg
    Department of Physics, University of Illinois at Urbana-Champaign.
  4. Ryan A Colyer
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  5. Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  6. Enrico Gratton
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  7. Christian Hellriegel
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  8. Oliver Holub
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  9. David M Jameson
    Department of Cell and Molecular Biology, University of Hawaii at Manoa
  10. Susana A Sánchez
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  11. Jay R Unruh
    Laboratory for Fluorescence Dynamics, University of California, Irvine.
  12. Paul W Wiseman
    Department of Physics and Chemistry, McGill University, Montreal, Canada.

Award Winners

Six registration waivers, sponsored by the Cell Migration Consortium and ISS, Inc. (Champaign, IL), have been awarded to the following students:

  1. Sohashank Bharill
    University of North Texas Health Science Center, Biochemistry and Molecular Biology, Fort Worth, TX.
  2. Sergi Padilla
    Institute Jacques Monod, Cellular Biology, Paris, France.
  3. Isabell Pechtl
    University of Edinburgh, Chemistry, Edinburgh, UK.
  4. Pabak Sarkar
    University of North Texas Health Science Center, Molecular Biology and Immunology, Fort Worth, TX.
  5. Nick Watson
    University of Louisville, Pharmacology and Toxicology, Louisville, KY.
  6. Leor Weinberger
    Princeton University, Molecular Biology, Princeton, NJ.

Paid Attendees

  1. Lorenzo Albertazzi
    Scuola Normale Superiore, Biophysics, Pisa, Italy.
  2. Rachel Williams Bailey
    Brigham Young University, Physiology and Developmental Biology, Provo, UT.
  3. John D Bell
    Brigham Young University, Physiology and Developmental Biology, Provo, UT.
  4. Jonathan Brewer
    University of Southern Denmark, BMB/MEMPHYS, Odense, Denmark.
  5. Ron S Broide
    Allergan Inc, Biological Science, Irvine, CA.
  6. Christof Buehler
    Novartis Institutes for BioMedical Research, Discovery Technologies - Innovative Screening Technologies, Vienna, Austria.
  7. Brian Cai
    Allergan Inc, Biological Sciences, RD3-3B, Irvine, CA.
  8. Andrea Callegari
    Scuola Normale Superiore, Biophysics, Pisa, Italy.
  9. Marcella Gilmore
    Allergan Inc, Biological Sciences, RD3-3B, Irvine, CA.
  10. Ravi Jasuja
    Boston University School of Medicine, Physiology and Biophysics, Boston, USA.
  11. David A Johnson
    University of California, Riverside, Biomedical Sciences, Riverside, CA.
  12. Donald C Lamb
    Ludwig-Maximilians-Universität München, Physical Chemistry, Munich, Germany.
  13. Park Lee
    University of Illinois at Chicago, Chemistry, Chicago, IL.
  14. Zhiwei Li
    Allergan Inc, Biological Science, Irvine, CA.
  15. Nikita Malavia
    University of California, Irvine, Biomedical Engineering, Irvine, CA.
  16. Kaustav Nandy
    National Cancer Institute / SAIC - Frederick, Image Analysis Lab, Frederick, MD.
  17. Jennifer Nelson
    Brigham Young University, Physiology and Developmental Biology, Provo, UT.
  18. Erin D Olson
    Brigham Young University, Physiology and Developmental Biology, Provo, UT.
  19. Rajesh Ramachandran
    The Scripps Research Institute, Cell Biology, La Jolla, CA.
  20. Nina K Reitan
    Norwegian University of Science and Technology, Physics, Trondheim, Norway.
  21. Justin Ross
    University of Hawaii, Cell and Molecular Biology, Honolulu, HI.
  22. Kenneth E Sawin
    University of Edinburgh, Wellcome Trust Centre for Cell Biology, Edinburgh, United Kingdom.
  23. Parijat Sengupta
    Stony Brook University Medical Center, Physiology and Biophysics, Stony Brook, NY.
  24. Malathi Srivatsan
    Arkansas State University, Biological Sciences/ABI, Jonesboro, AR.
  25. Nicole Wakida
    University of California, Irvine, Developmental and Cell Biology, CA.
  26. Robert Walder
    University of California, Irvine, Physics and Astronomy, Irvine, CA.
  27. Mark Washburn
    Allergan Inc, Biological Sciences, RD3-3B, Irvine, CA.
  28. Janice Yoshizawa
    University of California, Irvine, Molecular Biology and Biochemistry, Irvine, CA.
  29. Tiefeng You
    ISS Inc., Champaign, IL.
  30. Moreno Zamai
    Scientific Institute, Fondazione Centro San Raffaele, Milan, Italy.
  31. Shaohua Zhou
    University of California, Irvine, Developmental and Cell Biology, Irvine, CA.