Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Flyer

LFD Workshop Flyer

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

Carl Zeiss Inc.

Center for Complex Biological Systems

CMC Logo

Globals Software

University of California, Irvine

Group Photo

Group Photo

Notes

  • Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
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Abbreviations

  • FCS: Fluorescence Correlation Spectroscopy
  • FLIM: Fluorescence Lifetime Imaging
  • FRET: Förster Resonance Energy Transfer
  • GFP: Green Fluorescent Pasta
  • ICS: Image Correlation Spectroscopy
  • N&B: Number and Brightness
  • pCF: Pair Correlation Function
  • PCH: Photon Counting Histogram
  • PSF: Point Spread Function
  • PT: Particle Tracking
  • RICS: Raster Image Correlation Spectroscopy
  • STICS: Spation-Temporal Image Correlation Spectroscopy
  • more...

5th LFD Workshop (2010)

The "5th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 25–29, 2010, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.

Lecture Recordings

Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.

Agenda and Downloads

Monday, October 25, 2010

Tuesday, October 26, 2010

Wednesday, October 27, 2010

  • Breakfast
  • 09:00-09:45 – Lecture 7. Klaus Hahn: Biosensors and computational tools PDF
  • 09:45-10:30 – Lecture 8. Klaus Hahn: Recent developments: protein manipulation in vivo.
  • Break
  • 11:00-11:45 – Lecture 9. Enrico Gratton: Ratiometric FRET imaging PDF
  • 11:45-12:15 – Lecture 10. Michelle Digman: Cross-RICS and cross-N&B PDF
  • 12:15-12:45 – Fellowship talks
    • Carlos Alberto Marques de Carvalho: Shedding light on the entry of a new world alphavirus into host cells.
    • Julia Gunzenhäuser: Quantitative analysis of HIV-1 Gag clusters and the impact of fluorescently labelled Gag.
    • Moussa Barhoum: Utilizing spot size analysis (SSA) in investigation of single molecule trajectories.
  • Lunch
  • 14:00-17:00 – Computer based training on biosensors and FLIM. Download computing tutorials PDF and data files ZIP
  • 17:00-17:45 – Special lecture 2. Kerry Hanson: Glowing skin: how two-photon fluorescence imaging is improving sun protection.
  • 18:15-20:00 – Banquet dinner.

Thursday, October 28, 2010

Friday, October 29, 2010

  • Breakfast
  • 09:00-12:15 – Laboratory training on RICS and FLIM. Download laboratory tutorials PDF
  • 12:15-12:45 – Closure
  • Lunch

Lecturers

  1. Francesco Cardarelli
    Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
  2. Hongtao Chen
    Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
  3. Michelle A Digman
    Optical Biology Core Facility, University of California, Irvine, USA
  4. Enrico Gratton
    Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
  5. Klaus Hahn
    Department of Pharmacology, University of North Carolina at Chapel Hill, USA
  6. Kerry Hanson
    Department of Chemistry, University of California, Riverside, USA
  7. Elizabeth Hinde
    Optical Biology Core Facility, University of California, Irvine, USA
  8. David M Jameson
    Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA
  9. Luca Lanzanò
    Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
  10. Susana A Sánchez
    Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
  11. Chiara Stringari
    Laboratory for Fluorescence Dynamics, University of California, Irvine, USA

Registrants

  1. Moussa Barhoum
    Chemistry, University of Utah, Salt Lake City, UT
  2. Carol Bratt
    Dental Research, University of Iowa, Iowa City, IA
  3. Madeline Burgoyne
    Biomedical Science, Neurobiology and Anatomy, University of Texas, Houston, TX
  4. Carlos Alberto Marques de Carvalho
    Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil
  5. Eva Arnspang Christensen
    MEMPHYS Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark
  6. Rose Ellen Dixon
    Physiology and Biophysics, University of Washington, Seattle, WA
  7. Patton Garay
    Biological Sciences, Allergan Inc., Irvine, CA
  8. Harry Gill
    Phyiology and Biophysics, Case Western Reserve University, Cleveland, OH
  9. Julia Gunzenhäuser
    Laboratoire de Biophysique Expérimenta, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland
  10. Christopher Hammang
    School of Natural Sciences, University of Western Sydney, Sydney, Australia
  11. Yanfei Jiang
    Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO
  12. Ajit Joglekar
    Biology, University of North Carolina, Chapel Hill, NC
  13. Sander de Jong
    Research and Development, Lambert Instruments, Roden, The Netherlands
  14. Daichi Kamiyama
    Biology, University of Miami, Miami, FL
  15. Yujin Kim
    Cellular Biochemistry, Max-Planck-Institut für Biochemie, Martinsried, Germany
  16. Krzysztof Krawiec
    Faculty of Physics, Division of Biophysics, Warsaw University, Warszawa, Poland
  17. Pei-I Ku
    Physics, University of Utah, Salt Lake City, UT
  18. Moshe Levi
    Renal Diseases and Hypertension, Health Sciences Center, University of Colorado at Denver, Aurora, CO
  19. M Pilar Lillo
    Quimica Física Biológica, Inst. Quimica Fisica, CSIC, Madrid, Spain
  20. Xiangyi Luo
    Physics/Biology, University of Utah, Salt Lake City, UT
  21. Omid Masihzadeh
    Bioengineering, University of Colorado at Denver, Aurora, CO
  22. Joseph Mazurkiewicz
    Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY
  23. Radu Moldovan
    Advanced Light Microscopy Core Facility, School of Medicine, University of Colorado at Denver, Aurora, CO
  24. Rajesh Ramachandran
    Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH
  25. Justin Ross
    Cell and Molecular Biology, University of Hawaii, Honolulu, HI
  26. Fabrizio Salomone
    Laboratorio NEST, Scuola Normale Superiore di Pisa, Pisa, Italy
  27. Diana Schlamadinger
    Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA
  28. Xiaolin Tang
    Physics/Biology and Astronomy, University of Utah, Salt Lake City, UT
  29. Dudley Williams
    Biological Sciences, Allergan Inc., Irvine, CA
  30. Ding Xu
    Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA
  31. Melanie Pribisko Yen
    Chemistry, California Institute of Technology, Pasadena, CA
  32. Tiefeng You
    ISS Inc., Champaign, IL