Theodore L. Hazlett
Curriculum Vitae

Laboratory for Fluorescence Dynamics • Department of Physics
University of Illinois at Urbana-Champaign
1110 W. Green St. • Urbana, IL 61801-3080
E-Mail: thazlett@uiuc.edu
Telephone: (217) 244-5620 • Telefax: (217) 244-7187

Personal

Born: Pittsburgh, PA (August 28, 1957)

Education

Employment

Teaching Activities

Invited Lectures

Professional Societies

Scientific Journal and Manuscript Reviews

Publications

  1. N.Y. Lee, T.L. Hazlett and J.G. Koland. Structure and dynamics of the epidermal growth factor receptor C-terminal phosphorylation domain. Protein Sci. (in press) (2006)
  2. Tyler, K.M., K.M., Toriello, C.L. Olson, J.A. Cieslak, A. Fridberg, K.Y. Buchanan, E. Gratton, T.L. Hazlett, and DM Engman. Lipid Raft Association is a Mechanism for Flagellar Membrane Targetting. PNAS (submitted).
  3. Tricerri MA, Toledo JD, Sanchez SA, Hazlett TL, Gratton E, Jonas A, Garda HA. Visualization and analysis of apolipoprotein A-I interaction with binary phospholipid bilayers. J. Lipid Res. 46(4), 669-78. (2005).
  4. Hazlett, T., Q. Ruan, S. Tetin. Application of Fluorescence Correlation Spectroscopy to Hapten-Antibody Binding. In Protein – Ligand Interactions (Ed. UG Nienhaus), Humana Press (2005).
  5. Tetin, S., and T. Hazlett. Measuring Antibody Affinity Using Fluorescence in Measuring Immunity: The Immunologic Surrogates Handbook, First Edition. (2005).    
  6. Barcellona, M. L., S. Gammon, T. Hazlett, M. Digman and E. Gratton.  Polarized fluorescence correlation spectroscopy of DNA-DAPI complexes.  Microscopy Research and Technique  65(4-5), 205-217 (2004).
  7. Czeslik, C., G. Jackler, T. Hazlett, E. Gratton, R. Steitz, A. Wittemann and M. Ballauff. Salt-induced protein resistance of polyelectrolyte brushes studied using fluorescence correlation spectroscopy and neutron reflectometry.  Phys . Chem. Chem. Phys . 6, 5557–5563 (2004).
  8. Janosch, S., C. Nicolini, B. Ludolf, C. Peters, M. Volkert, T.L. Hazlett, E. Gratton, H. Waldmann, and R. Winter. Partitioning of Dual-Lipidated Peptides into Membrane Microdomains - Lipid Sorting vs. Peptide Aggregation. J. Amer. Chem. Soc. 126(24), 7496-7503 (2004).
  9. Czeslik, C., R. Jansen, M. Ballauff, A. Wittmann, C. A. Royer, E. Gratton and T. Hazlett.  Mechanism of protein binding to spherical polyelectrolyte brushes studied in situ using two-photon excitation fluorescence fluctuation spectroscopy.  Phys. Rev. E  69, 021401-021409 (2004).
  10. Bell, J.D., S.A.Sanchez, and T.L. Hazlett. Use of Liposomes to Study Phospholipase A2 Activity. Methods in Enzymology, Liposomes Part E, Vol. 361 (N. Duzgunes, Ed.) (2003).
  11. Campanini, B., S. Raboni, S. Vaccari, L. Zhang, P.F. Cook, T.L. Hazlett, A. Mozzarelli, and S. Bettati. Surface-exposed Tryptophan Residues Are Essential for O-Acetylserine Sulfhydolase Structure, Function and Stability. J. Biol. Chem. 37511-37519 (2003).
  12. Czeslik, C., C. Royer, T. Hazlett and W. W. Mantulin.   Reorientational dynamics of enzymes adsorbed on quartz:  A temperature dependent time-resolved TIRF anisotropy study. Biophysical J. 84, 2533-2541 (2003).
  13. Bagatolli, L.A., S.A.Sanchez, T.L. Hazlett, And E. Gratton. Giant Vesicles, Laurdan and Two-Photon Fluorescence Microscopy: Direct Evidence of Lipid Lateral Separation in Bilayers.  Methods in Enzymology 360, 481-500 (2003).
  14. Patel, R. C; U. Kumar, D. C. Lamb, J. S. Eid, M. Rocheville, M. Grant, A. Rani, T. Hazlett, S. C. Patel, E. Gratton, Y. C. Patel. Ligand binding to somatostatin receptors induces receptor-specific oligomer formation in live cells. Proc. of the National Academy of Sciences of the USA 99(5), 3294-3299 (2002).
  15. Bettati, S., B.Campanini, S. Vaccari, A. Mozzarelli, G. Schianchi, T. L. Hazlett, and E. Gratton. Unfolding of pyridoxal 5'-phosphate-dependent O-acetylserine sulfhydrylase probed by time-resolved tryptophan fluorescence. Biochim. Biophys. Acta 1596 (1), 47-54 (2002).
  16. Best, K.B., Ohran, A.J., Hawes, A.C., Hazlett, T.L., Gratton, E., Judd, A.M., Bell, J.D. Relationship between Erythocyte Membrane Phase Properties and Susceptibility to Secretory Phospholipase A2 Biochemistry 41(47), 13982-13988 (2002).
  17. Fahsel, S., E.-M. Pospiech, M. Zein, T. L. Hazlet, E. Gratton, and R. Winter.  Modulation of Concentration Fluctuations in Phase-Separated Lipid Membranes by Polypeptide Insertion. Biophys. J. 83, 334-344 (2002).
  18. Sanchez, S. A., L. A. Bagatolli, E. Gratton and T. L. Hazlett. A Two Photon View of an Enzyme at Work: C. atrox Venom PLA2 Interaction with Single-Lipid and Mixed-Lipid Giant Unilamellar Vesicles. Biophys. J. 82 2232-2243 (2002).
  19. Jasuja R., T.L. Hazlett, M.K. Helms, S.H. Lee, D.M. Jameson and R.W. Larsen. Temperature Dependence of Photoinduced Electron Transfer within Self-Associated Porphyrin: Guanine Monophosphate Complexes. Chem. Phys. Let. 350, 515-521 (2001).
  20. Sanchez, S. A., Y. Chen, J. D. Mueller, E. Gratton, T. L. Hazlett. Solution and Interface Aggregation States of C. atrox Venom Phospholipase A2 by 2-Photon Excitation Fluorescence Correlation Spectroscopy. Biochemistry, 40, 6903-6911 (2001) .
  21. Bettati, S., S. Benci, B. Campanini, S. Raboni, G. Chirico, S. Beretta, K. D. Schnackerz, T. L. Hazlett, E. Gratton, and A. Mozzarelli. Role of Pyridoxal 5'-Phosphate in the Structural Stabilization of 0-Acetylserine Sulfhydrylase. J.Biol.Chem., 275, 40244-40251 (2000).
  22. Tetin, S. Y. and T. L. Hazlett. Optical Spectroscopy in Studies of Antibody-Hapten Interactions. Methods 20, 341-36 (2000)1.
  23. Davidson, W. S., K. Arnvig-McGuire, A. Kennedy, J. Kossman, T. L. Hazlett and A. Jonas. Structural Organization of the N-Terminal Domain of Apolipoprotein A-I: Studies of Tryptophan Mutants. Biochemistry 38, 14387-14395 (1999).
  24. Gilmore, A. M., V. P. Shinkarev, T. L. Hazlett, and Govindjee. Quantitative Analysis of the Effects of Intrathylakoid pH and Xanthophyll Cycle Pigments on Chlorophyll a: Fluorescence Lifetime Distributions and Intensity in Thylakoids. Biochemistry 37(39) 13582-13593 (1998).
  25. Helms, M. K., T. L. Hazlett, H. Mizuguchi, C. A. Hasemann, K. Uyeda, and D. M. Jameson. Site-Directed Mutants of Rat Testis Fructose-6-Phosphate, 2-Kinase: Fructose-2,6-Bisphosphatase Localization of Conformation Alterations Induced by Ligand Binding. Biochemistry 40, 14057-14064 (1998).
  26. Brune, M., J. L. Hunter, S. A. Howell, S. R. Martin, T. L. Hazlett, J. E. T. Corrie, and M. R. Webb. Mechanism of Inorganic Phosphate Interaction with Phosphate Binding Protein from Escherichia coli. Biochemistry 37, 14057-14064 (1998).
  27. Sanchez, S. A., T. L. Hazlett, J. E. Brunet, and D. M. Jameson. Aggregation States of Mitochondrial Malate Dehydrogenase. Protein Science 7, 1-6 (1998).
  28. Gilmore, A. M., T. L. Hazlett, P. G. Debrunner and Govindjee. Photosystem II Cholorophyll Fluorescence Lifetimes are Independent of the Antenna Size Differences between Barley Wild-Type and Chlorina Mutants; Comparison of Xanthophyll-Cycle Dependent and Photochemical Quenching. Photosynth. Res. 48, 171-187 (1996).
  29. Gilmore, A. M., T. L. Hazlett, P. G. Debrunner, and Govindjee. Comparative Time-Resolved Photosystem II Chlorophyll Fluorescence Analyses Reveal Distinctive Differences between Photoinhibitory Reaction Center Damage and Xanthophyll Cycle-Dependent Energy Dissipation. Photochem. Photobiol. 64, 552-563 (1996).
  30. Gilmore, A. M., T. L. Hazlett, O. Bjorkman, and Govindjee. Xanthophyll Cycle Dependent Non-Photochemical Quenching of Chlorophyll Fluorescence at Low Physiological Temperatures, Proc. 10th International Congress of Photosynthesis (Mathis, P., ed. (1996)).
  31. Davidson, W. S., T. Hazlett, W. W. Mantulin and A. Jonas. The Role of Apolipoprotein AI Domains in Lipid Binding. Proc. Natl. Acad. Sci., US, 93, 13605-13610.
  32. Watson, B. S., T. L. Hazlett, J. F. Eccleston, C. Davis, D. M. Jameson, and A. E. Johnson. (1995) Macromolecular Arrangement in the Aminoacyl-tRNA-Elongation Factor Tu-GTP Ternary Complex. A Fluorescence Energy Transfer Study. Biochemistry 34, 7 904-7912 (1996).
  33. Bernsdorff, C., R. Winter, T. L. Hazlett, and E. Gratton. Influence of Cholesterol and b-Sitosterol on the Dynamic Behaviour of DPPC as Detected by TMA-DPH and PyrPc Fluorescence: A Fluorescence Lifetime Distribution and Time-Resolved Anisotropy Study, Ber. Bunsenges. Phys. Chem. 99, 1479-1488 (1995).
  34. Gilmore, A. M., T. L. Hazlett, Govindjee.  Xanthophyll Cycle Dependent Quenching of Photosystem II Chlorophyll a Fluorescence: Formation of a Quenching Complex with a Short Fluorescence Lifetime. Proc. Nat. Acad. of Sci. USA 92, 2273-2277(1995).
  35. Hazlett, T.L., K. J. M. Moore, P. N. Lowe, D. M. Jameson, and J. F. Eccleston. Solution Dynamics of p21ras Proteins Bound with Fluorescence Nucleotides: A Time-Resolved Fluorescence Study, Biochemistry 32, 13575-13583 (1993).
  36. Tetin, S.Y., C. A. Rumbley, T. L. Hazlett, and E. W. Voss, Jr. Elucidation of Anti-ssDNA Autoantibody BV 04-01 Binding Interactions with Homonucleotides. Biochemistry 32, 9011-9017 (1993) .
  37. Hazlett, T. L., T. Hagashijima, and D. M. Jameson. Examination of Elongation Factor Tu for Aluminum Fluoride Binding Sites using Fluorescence and 19F-NMR Methodologies. FEBS Lett. 278, 225-22 (1991)8.
  38. Jameson, D. M. and T. L. Hazlett. Time-Resolved Fluorescence in Biology and Biochemistry, in Biophysical and Biochemical Aspects of Fluorescence Spectroscopy (Dewey, Ed.) Plenum Press, New York (1991).
  39. Hazlett, T. L.,R. A. Deems, and E. A. Dennis. Activation, Aggregation, Inhibition and the Mechanism of Phospholipase A2, in Biochemistry. Molecular Biology and Physiology of Phospholipase A2 and Its Regulatory Factors, (Mukheerjee, Ed.) Plenum Press, New York (1990).
  40. Hazlett, T. L., A. J. Johnson, and D. M. Jameson. Time-Resolved Fluorescence Studies on the Ternary Complex Formed Between Bacterial Elongation Factor Tu, Guanosine 5'-Triphosphate, and Phenylalanyl-tRNAPhe. Biochemistry 28, 4109-4117 (1989).
  41. Hazlett, T. L. and D. M. Jameson. Time-Resolved Fluorescence Studies on Components of the Prokaryotic Protein Elongation System. Time-Resolved Laser Spectroscopy in Biochemistry: Proceedings of SPIE 209, 412-419 (1988).
  42. Hazlett, T. L. and E. A. Dennis. Lipid-Induced Aggregation of Phospholipase A2: Sucrose Density Gradient Ultracentrifugation and Cross-Linking Studies. Biochim. Biophys. Acta. 961, 22-29 (1988).
  43. Jameson, D. M., and Hazlett, T. L. Fluorescence Investigations on the Elongation Factor Tu System, in Fluorescence Biomolecules: Methodologies and Applications, (Jameson and Reinhart, Eds.) Plenum Press, New York (1988).
  44. Hazlett, T. L. and E. A. Dennis. Effect of Phospholipids on Fluorescence Polarization and Lifetimes of Fluorescein-Labeled Phospholipase A2. Biochim. Biophys. Acta 958, 172-178 (1987)..
  45. Hazlett, T. L. and E. A. Dennis. Aggregation Studies on Fluorescein-Coupled Cobra Venom Phospholipase A2. Biochemistry 24, 6152-6157 (1985).
  46. Dennis, E. A., T. L. Hazlett, R. A. Deems, M. I. Ross, and R. J. Ulevitch. Phopholipases in the Macrophage, in Prostaglandins, Leukotrienes, and Lipoxins, IV International Washington Spring Symposium, Plenum Press, New York, (Bailey, Ed.), 213-220 (1985).
  47. Hazlett, T. L. and E. A. Dennis. Affinity Chromatography of Phospholipase A2 from Naja naja naja (Indian Cobra) Venom. Toxicon 23, 457-466 (1985).
  48. Young, A. M. and T. L. Hazlett. The Effect of Salinity and Temperature on the Larva Development of Clibanarius vittatus (Bosc) (Crustacea: Decapoda: Diogenidae). J. Exp. Mar. Biol. Ecol. 34, 131-141 (1978).

Abstracts Not Published in Full

  1. Hazlett, T.L. and E. Gratton (2006) Characterization of Particle Angular Fluctuations by Intensity and Polarization Trajectory Plots. Biophys J. 90.
  2. Hazlett, T.L., and E. Gratton, (2004) Photon Counting and Analog Data Acquisition in Fluorescence Correlation Spectroscopy, Issues of Sensitivity and Dynamic Range. Biophys J. 86, 157a.
  3. Hazlett, T.L. and E. Gratton (2003) Punctuated Lipid Mass Loss During Enzyme-Mediated Lipid Hydrolysis on Giant Unilamellar Veiscles. Biophys. J. 84.
  4. Tetin, S.Y., K. M. Swift, T.L.Hazlett, J. Herron, and E. D. Matayoshi. (2002) Studying Interactions of Anti-Fluorescyl Antibodies with Fluorescein Derivatives at the Single Molecule Level. Biophys. J. 82, 334a.
  5. Hazlett, T.L., S.A. Sanchez and E. Gratton. (2002) Exploring Transient Binding and Diffusion of Molecules on Bilayer Surfaces with Two-Photon Fluorescence Microscopy. Biophys. J. 82, 546a.
  6. Tyler, K.M., K.T. Buchanan, K.M.Torelli, J. Ceislak, T. Hazlett, E. Gratton and D.M. Engman. (2001) Liquid Ordered and Detergent Resistant Membranes – The Flagellar Connection.
  7. Sanchez, S.A., J. Mueller, T. Hazlett, & E. Gratton. (2001) Protein Dissociation under Pressure Using Fluctuation Correlation Spectroscopy. Biophys J., 80(1), A556.
  8. Sanchez, S., M. V. Yeditha, and T. Hazlett. (1998) The Influence of Cofactors and Environmental Conditions on the Monomer Exchange Rate Kinetics of a Dimeric Phospholipase A2. Biophys. J. 74(2), A173.
  9. Pilas, B., S. Sanchez, T. L. Hazlett, and G. Durack. (1999) Measurements of Cellular Chloride Concentrations with 6-Methoxy-n-Ethylquinolinium-Iodide using Conventional and Lifetime-Based Flow Cytometry. Biophys. J. 76, A449.
  10. Hazlett, T. L. and J. F. Eccleston. (1995) Pressure- and Salt-Induced Perturbations of the Neurofibromin*ras p21*GTP Ternary Complex. Biophys. J. 68, A70.
  11. Muller, J. D., C. D. Eng, S. Y. Tetin, T. L. Hazlett, E. W. Voss, Jr., and U. Nienhaus. (1994) Influence of Viscosity and Temperature on the Conformational Drift in Monoclonal Anti-Fluorescyl Antibodies (Mab) 4-420. Biophys. J. 66, A69.
  12. Tetin, S. Y., E. W. Voss, Jr., and T. L. Hazlett. (1993) Evidence for Conformation Drift in the Interaction of Antibodies with Haptens. Biophys. J. 66, A341.
  13. Cregar, L., K. Heldt, and T. L. Hazlett. (1991) Investigations into the Solution Dynamics of Phospholipase A2 using Phase and Modulation Fluorometry. Biomedical Sciences Symposium, John A. Burns School of Medicine, Univ. of Hawaii.

Theodore L. Hazlett