Oliver Holub |
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| ollihol at web.de Phone: +55 21 81 27 74 81 Permanent Address: |
Present Address: Laboratório de Biofísica
Química de Proteínas |
Education
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Publications
- Fluorescence lifetime imaging (FLI) in real-time - a new technique in photosynthesis research. Oliver Holub, Manfredo J. Seufferheld, Christoph Gohlke, Govindjee and Robert M. Clegg. Photosynthetica 38(4) 581-599, 2000. Download PDF (4.5 MB).
- Fluorescence Lifetime-resolved Imaging. Measuring lifetimes in an image. Why do it? How to do it. How to interpret it. Robert M. Clegg, Oliver Holub and Christoph Gohlke. Methods in Enzymology, Biophotonics; Part A (two-volume issue); Edited by Gerard Marriot and Ian Parker; Vol. 360 (361 A); Sect. C. Imaging Microscopy; Chap. 22; p. 509-542 (pp.680); ISBN 0-12-182263-X; Academic Press, San Diego 2003. Download PDF (8.6 MB)
- Binding of ethidium to yeast tRNAPhe : A new perspective on an old Bromide. Marc Tramier, Oliver Holub, John C. Croney, Takahisa Ishii, Steven E. Seifried and David M. Jameson. Fluorescence Spectroscopy, Imaging and Probes; Editors: R. Kraayenhof, A.J.W.G. Visser and H.C. Gerritsen; Springer-Verlag Berlin, Heidelberg, New York; 2002; p. 111-121; ISBN 3-540-42768-6; on the occasion of the "Seventh International Conference on Methods and Applications of Fluorescence Spectroscopy, Imaging and Probes", Amsterdam, The Netherlands, September 16-19, 2001. Download PDF (710 kB).
Posters
- Stereochemistry of DNA molecules by homo-energy transfer. Oliver
Holub and Robert M. Clegg. Presented at the "Second Conference
on Fluorescence Microscopy & Fluorescent Probes", Prague, Czechia,
April 9-12, 1997; and "42nd Annual Meeting of the Biophysical Society",
Kansas City, Missouri, February 22-26, 1998. Download
PDF (1.1 MB).
- Real-Time Fluorescence Lifetime-Resolved Imaging. Peter C. Schneider, Oliver
Holub, and Robert M. Clegg. Presented at the "Second Conference
on Fluorescence Microscopy & Fluorescent Probes", Prague, Czechia,
April 9-12, 1997. Download PDF (1.5 MB).
- Real-Time Fluorescence Lifetime-Resolved Images of 5-Aminolevulinic
Acid-Induced Protoporphyrin IX in Cells. Peter C. Schneider, Oliver
Holub, and Robert M. Clegg. Presented at the "42nd Annual Meeting
of the Biophysical Society", Kansas City, Missouri, February 22-26,
1998. Download PDF (2.4 MB).
- "Second order" sequence specificity of DAPI-binding to AT-regions
in DNA. Oliver Holub and Robert M. Clegg. Presented at the "43rd
Annual Meeting of the Biophysical Society", Baltimore, Maryland,
February 13-17, 1999. Download
PDF (430 KB).
- Want to know something about your fluorescent samples that optics
cannot resolve? Long working-distance stage-scanning instrument for Real-Time
Fluorescence Lifetime-Resolved Imaging. Oliver Holub, Christoph
Gohlke, Manfredo Seufferheld, Govindjee, and Robert M. Clegg. Presented
at the "44th Annual Meeting of the Biophysical Society",
New Orleans, Louisiana, February 12-16, 2000. Download
PDF (1.2 MB).
- New perspectives on an old bromide Ethidium binding to yeast tRNAPhe.
Marc Tramier, Oliver Holub, John Croney, Takahisa Ishii, Steven
E. Seifried, David M. Jameson. Presented at the "44th Annual Meeting
of the Biophysical Society", New Orleans, Louisiana, February 12-16,
2000. Download PDF (630 KB).
- Real-time fluorescence lifetime-resolved images of individual cells
of wild type and NPQ mutants of Chlamydomonas reinhardtii. Oliver
Holub, Manfredo Seufferheld, Christoph Gohlke, Robert M. Clegg and
Govindjee. Presented at the 9th International Conference on the Cell
and Molecular Biology of Chlamydamonas, Amsterdam, The Netherlands, May
21-26, 2000. Download PDF (1.1 MB).
- FlimFast - Fluorescence Lifetime Image Acquisition and Visualization
at Video-Rate. Christoph Gohlke, Oliver Holub, and Robert
M. Clegg. Presented at the "45th Annual Meeting of the Biophysical
Society", Boston, Massachusetts, February 17-21, 2001. Download
PDF (2.1 MB).
- Application of real-time fluorescence lifetime-resolved imaging in
photosynthesis: Studies of maize leaves (Zea Mays),
small mustard leaves (Arabidopsis thaliana), and of individual
wild-type and mutant cells of the green alga Chlamydomonas reinhardtii. Oliver
Holub, Manfredo J. Seufferheld, Christoph Gohlke, Govindjee, and
Robert M. Clegg. Presented at the "45th Annual Meeting of the Biophysical
Society", Boston, Massachusetts, February 17-21, 2001. Download
PDF (1.8 MB).
- Fast, Macroscopic Chlorophyll Fluorescence Lifetime Imaging of Apple Fruit Skin. Martin J. Vandeven, Oliver Holub, Christoph Gohlke, Govindjee, Roland Valcke, Marcel Ameloot, and Robert M. Clegg. Presented at the "46th Annual Meeting of the Biophysical Society", San Francisco, California, February 23-27, 2002.
Oral Presentations
- Fluorescence lifetime imaging at video rate - applications in photosynthesis.
Presented by Oliver Holub. Co-authored by M.J. Seufferheld, C. Gohlke,
Govindjee and R.M. Clegg. Presented at the "Annual Photosynthesis
Conference & Retreat", Robert Allerton House, Monticello, IL,
March 3-4, 2001.
- Real-time fluorescence lifetime-resolved imaging - why we do it, how
it's done and, applications for biology and medicine. Presented by
R.M. Clegg. Co-authored by Oliver Holub, C. Gohlke, S. Breusegem,
N. Barry and E. Gratton. Presented at "CYTO 2001 FLIM, FRET and
Multiphoton Microscopy"; St Catherine's College, University of Oxford,
July 1-3, 2001.
- Fluorescence lifetime imaging microscopy (FLIM) of non-photochemical quenching mutants of C. reinhardtii. Presented by Govindjee. Co-authored by Oliver Holub, M.J. Seufferheld, C. Gohlke, and R.M. Clegg. Presented at "Light stress and photosynthesis UV-B and visible light effects" (a satellite meeting for PS2001, The 12th International Congress on Photosynthesis); Heron Island Resort, Queensland, Australia, August 13-17, 2001.
Movies
- FlimFast - Fluorescence Lifetime Imaging at Video Rate: Demonstration of continuous fluorescence lifetime image measurements with concurrent analysis and visualization at 3-26 Hz. by Christoph Gohlke and Oliver Holub. Movie 1 2 3 4 (WMV8, 7-18 MB)
Oliver Holub, June 2003