Fluorescence Lifetime Phasor Plot Applet

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Legend:

• The green and red plots (—) are the observed decays in the green (donor) and red (acceptor) channels.
• The filled circles (●) are the phasors corresponding to these plots.
• The open circles (○) are the phasors of the donor alone/acceptor alone.

By Jay R Unruh, Laboratory for Fluorescence Dynamics, April 4, 2007.