Single plane illumination microscopy (SPIM) in an upright configuration allows for fast 3D imaging of single cells grown on glass coverslips, and of large, multicellular organisms such as zebrafish embryos.
The custom-built SPIM system at the LFD can simultaneously image two color channels, e.g. the nucleus (blue, H2B-EGFP) and mitochondria (green, TMRE) of live CHO-K1 cells (shown).
The Laboratory for Fluorescence Dynamics (LFD) is a national research resource center for biomedical fluorescence spectroscopy, supported by the National Institute of General Medical Sciences (NIGMS P41GM103540) divisions of the National Institutes of Health (NIH) and the University of California, Irvine (UCI).
The founder and Principal Investigator of the LFD is Enrico Gratton. The Co-Investigator is Michelle Digman.
The LFD's main activities are:
Read more about the LFD's activities, history, funding, and organization.
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