Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
LFD Workshop tutorials & lectures
- 15th LFD Workshop (2021)
- 14th LFD Workshop (2019)
- 13th LFD Workshop (2018)
- 12th LFD Workshop (2017)
- 11th LFD Workshop (2016)
- 10th LFD Workshop (2015)
- 9th LFD Workshop (2014)
- 8th LFD Workshop (2013)
- 7th LFD Workshop (2012)
- 6th LFD Workshop (2011)
- 5th LFD Workshop (2010)
- 4th LFD Workshop (2009)
- 3rd LFD Workshop (2008)
- 2nd LFD Workshop (2007)
- 1st LFD Workshop (2006)
SimFCS sponsor talks
Other tutorials
- pCF analysis of big images using Python
- LFD Colloquium notes, August and September 2016
Globals Software Tutorials
Tutorials for the Globals Software for Spectroscopy and Images, written by Enrico Gratton and other members of the Laboratory for Fluorescence Dynamics (LFD).
- Acquiring and calibrating FLIM files
- Creating NADH FLIM color coded images and performing region of interest image segmentation
- Reading the PicoQuant format PT3 for the analysis of FCS data
- Calibration of the B scale in the NB analysis using a Chroma slide
- Single point fluctuations analysis with the LSM880 Airy detector
- LFD-Globals-Software-Tutorials
- pCF cross-correlation
- L-STICS correlation function analysis
- iMSD analysis with scripts
- 2D pair correlation analysis
- Select arbitrary objects in images and save as sub images
- Determining the absolute concentration of NADH
- The Distance program
- Converting very large Zeiss LSM files into BIN files for spectral and 3D analysis
- Pair correlation for images (2D maps and connectivity maps)
- Reading and analyzing FBD files acquired with ISS Vista software
- Using the correlation of the B carpet
- Brightness-ACF and pCF analysis
- Spectral phasor analysis
- The RICS-scan analysis
- Analyzing tICS using exponentials or phasors
- Using the hand-draw tool to create masks
- Using PicoQuant PT3 format for FLIM
- The SimFCS64 file formats
- Digital output of the IOtech card
- Reading SDT image files for phasor analysis
- Using masks in the phasor analysis
- The FIT file format
- The iMSD screen and description of the algorithms
- Reading TIF files for Phasor-FLIM analysis
- Calculation and display of z-phasors
- The compare-next option in the phasor FLIM analysis
- Using the batch option in the RICS screen
- Using intensity masks for the FLIM analysis
- Using multiple points in a pixel or multiple lines in a frame for data acquisition using the IOtech 3000 card
- Reading text spectral files and calculate the spectral phasor
- Using the diffusion phasors for the analysis of tICS
- Using the concentration track bar to calculate the concentration of ions or pH in the phasor plot
- N&B analysis using Gaussian cursors
- New file structure for the FLIMbox FBD format
- Code for the trap formula and j-analysis
- Calculating volumes from LSM 3D image stacks
- Deriving the laser frequency from PicoQuant BIN files
- Reading the PicoQuant BIN file
- Using phasors to analyze single point FCS images using the multiple dwell
- Opening PicoqQant line scans in PT3 format
- 3D reconstructions of z-stacks and phasor masks
- Reading SPC files
- Reading STD file for spectral detector
- GP imaging
- Reading SDT files
- iMSD scan analysis
- Analyzing TIRF BIN files for iMSD
- Using separate stage drivers for x-y and z motion
- Changing laser power during the z-stack
- Simulating flow
- New RICS scan-image
- Using the program FORMULA to display 3D surfaces and masks from phasor analysis
- Processing data acquired in the edge tracking mode
- Simulating rotating sticks
- Using the sensitivity parameter in particle tracking
- Reading phosphorescence files
- Correcting photon counts due to detector pile up
- The new scanner waveform
- Using ramps in the tracking screen
- Spectral phasor simulation
- Moving averages columns in the Scanning FCS screen
- Phasor analysis with a heterogeneous reference
- The IOtech 3001 PCI card
- Segmentation of trajectories according to the J parameter
- Reading RAW files from the LFD-Lambert acquisition program
- Reading Lambert camera files in RAW format and convert to phasor
- Brightness and number cross-variance
- Using Becker & Hickl data for phasor analysis
- Calculating the spectral phasor
- Autocorrelation and pair correlation analysis of a line scan
- New intensity scales for the Phasors page
- Using weights in FCS fits
- Reading FLIM line data from the Olympus FV1000
- Dividing the huge vector in two parts when tracking 2 particles simultaneously
- Using a single file for frequency domain fitting of intensity and anisotropy decay
- Using the cell phasor in a time sequence
- Processing FBD files averaging a number of files
- Acquiring a number of frames using the FLIMbox screen
- Using the ISS TDU unit
- Hardware programming of the ISS motor controller box
- BIFL analysis
- Displaying two objects in the modulation tracking screen
- The BIFL page
- Changes to the track screen
- Calibrating galvo and piezo
- Calibrating the phase and amplitude of the scanner to compensate for its slow response at high frequency
- Processing FLIM line files
- Function readthePICOQUANTfile
- Analyzing N&B in AVI movies
- Analyzing FCS and PCH raw data from the Zeiss Confocor 2
- FLIM-FRET phasor analysis of FBD files
- Extracting ramp segments in MT nanoimaging
- Decoding the FBD file
- FLIM measurements using the Lambert camera
- Using the Ti Sapphire laser and the FLIMbox
- Fitting time domain anisotropy data in Globals for Spectroscopy
- Calibrating the time response of the scanner
- Using the FLIMbox with the image acquire screen
- Using linked cursors in the Phasor plot and obtain the histogram of fractional intensities
- Straighten up lines in scanning FCS
- Analyzing single point time-time domain data using phasors
- Reading PicoQuant PT3 files for FCS and lifetime FCS
- Analyzing anisotropy decay using associative models in Globals for Spectroscopy
- Simulating cylinders of elliptical shape for 3D tracking
- Simulating actin flow in cell protrusion
- Functions in the Scanning FCS analysis page for the 3D reconstruction using modulation tracking
- Assessing the effect of background counts on the determination of the correct radius of a cylinder using the MT method
- Merging images in the RICS and scanning FCS screen
- Analyzing cell protrusions using Modulation Tracking
- Simulating regions of different diffusion for testing pCF
- Reading and processing image frames larger than 256 in the FLIMbox screen
- Determining the laser repetition rate for the FLIMbox when using the TiSapphire laser
- Reading FCS files (ISS format) for FCS and images. RICS and N&B with the Leica SP5
- Measure MSD using correlation
- Reading M format ASCII files in Globals
- Reading BIN files from the Olympus TIRF
- GP analysis of TIRF dual view images
- F-FCS, Lifetime resolved FCS using the FLIMbox card
- Calibrating the 3-axis card after January 15, 2010
- Tracking surfaces (villi) in 3D
- Tracking surfaces in xz and yz planes
- Analyzing TIRF data
- FLIMbox connections
- Simplified alignment procedures for the ALBA system
- BIFL analysis
- External clock for synchronization
- Improvements to GP analysis
- Calculating and displaying GP images and histograms
- Code for the N&B functions
- Nanometer resolution Circular Tracking (nrCT)
- Extracting lines in an image for pair correlation analysis
- Fitting autocorrelation curves using the anomalous diffusion term
- Selecting ROI in the phasor analysis
- Saving mask and histogram images in the phasor screen
- Exporting N&B primitives for further display and analysis
- Installing the FLIMbox in a new computer
- The multiple-cursor selector for phasors and multiple files processing
- FLIM with multiple channels
- Temporal image correlation spectroscopy (tICS)
- GP images selection using the FRET screen
- The FLIMbox as of January 27, 2009
- Calibrating the ALBA box
- Phasor analysis
- Reading PicoQuant BIN files
- The FLIMbox as of November 11, 2008
- N&B analysis using the analog mode in the LSM510
- Particle tracking for images
- FRET intensity image calculations
- Using the ISS ALBA with the two-photon laser
- Using the FLIMbox with the ISS ALBA microscope
- Reading Lambert instrument files
- Reading PixeLINK camera files in RAW format
- Cross-N&B
- ALBA box commands
- Using frame freezing for FLIMbox acquisition with the Zeiss 510
- Using the SCAN function in the RICS page
- GP image displays in the RICS screen
- RICS cross-correlation
- Reading RAW files in the RICS screen
- RICS cross-correlation analysis
- Using the Zeiss Confocor 3 with the FLIMbox
- Particle Tracking
- Using the tracking routine with an image stack
- Using calibration equations in FLIM
- Concepts in Fluorescence Correlation Spectroscopy
- Simulating FCS and particle trajectories
- Phasor autofluorescence subtraction
- Line scan analysis
- N&B analysis for the line scan of Paxillin-EGFP
- GP image analysis
- The brightness and N button in the RICS screen
- FLIM-FRET phasor analysis of files from the Lambert instrument
- The time derivative and velocity buttons
- Calibrating the PSF volume for PCH analysis