11th Weber Symposium (2023)

Agenda

Sunday, January 8, 2023

• 3:00-6:00 PM – Registration. Poster and exhibit setup.
• 7:30 PM – Welcome: Leonel Malacrida, David M Jameson and Enrico Gratton.
• 7:45 PM – Opening lecture by David M Jameson: The seminal contributions of Gregorio Weber to modern fluorescence spectroscopy and to protein chemistry.
• 8:45 PM – Cocktail reception.

Monday, January 9, 2023

• 7:30 AM – Breakfast at dinning room, followed by posters, and exhibits.
• Chair: Gregory Reinhart
• 9:00 AM – Enrico Gratton: Multiplexing spectra and lifetime in 32 channels simultaneously.
• 9:30 AM – Diego Presman: Insights on transcription factors and chromatin dynamics through single-molecule tracking in live cells.
• 10:00 AM – Francesco Cardarelli: Once in a (fluorescence) lifetime: from synthetic identity to biological function of nanoencapsulated drugs in biomedical applications.
• 10:30 AM – Break, posters and exhibits.
• Chair: Leonel Malacrida
• 11:15 AM – Jay Knutson: Fluorescence Lifetime Microscopy : Metabolic Signaling and Analysis.
• 11:45 AM – Luca Lanzanò: Lifetime-based super-resolution and its application to a model of oncogenesis.
• 12:15 PM – Jörg Enderlein: Metal- and Graphene-Induced Energy Transfer Imaging.
• 12:30 PM – Lunch.
• Chair: David Jameson
• 2:15 PM – Melike Lakadamyali: Super-resolution imaging of chromatin structure and dynamics in health and disease.
• 2:45 PM – Francisco Barrantes: The individual molecule and its tribe, the nanocluster: the case of the nicotinic acetylcholine receptor.
• 3:15 PM – Srigokul Upadhyayula: Making the invisible visible: imaging subcellular dynamics in multicellular systems.
• 3:45 PM – Break with posters and exhibits.
• Chair: Francesco Cardarelli
• 4:30 PM – Amitabha Chattopadhyay: Cholesterol-dependent Endocytosis and Trafficking of GPCRs: Implications in Pathophysiology and Therapeutics.
• 5:00 PM – Hernán Grecco - Argentina - From experiments to model and back: activity flow in the apoptotic network.

Tuesday, January 10, 2023

• 7:30 AM – Breakfast at dinning room, followed posters, and exhibits.
• Chair: Alberto Diaspro
• 9:00 AM – Fernando Stefani: RASTMIN: an alternative to MINFLUX that enables nanometre resolution in a laser-scanning (confocal) microscope.
• 9:30 AM – Ilaria Testa: From STARSS: measuring rotational diffusivity with reversible photo-switching.
• 10:00 AM – Adán Guerrero: Enhancing resolution within a single imaging frame.
• 10:30 AM – Break, posters and exhibits.
• Chair: Jay Knutson
• 11:15 AM – Rafael Piestun: Optimizing Super-resolution Localization Microscopy: Three Dimensions, Dense Scenes, Fast drift-less acquisition, and Multicolor/Multispectral Imaging.
• 11:45 AM – Don Lamb: Measuring protein dynamics using smFRET.
• 12:15 PM – Martin Hof: Protein hydration and dynamics seen by fluorescence: TDFS and PET-FCS.
• 12:45 PM – Free time.
• Chair: Leonel Malacrida
• 2:00 PM – Valeria Levi: An unexpected partnership between SOX2 and the Glucocorticoid receptor: nothing to do together but something (unknown) in common.
• 2:30 PM – Sua Myong: G-quadruplex as a genetic switch in transcription and translation.
• 3:00 PM – MLuke Lavis: Designing brighter dyes for advanced fluorescence microscopy and beyond.
• 3:30 PM – Break, posters and exhibits.
• Chair: David M Jameson
• 4:00 PM – Elizabeth Hinde - Australia - Fluorescence fluctuation spectroscopy of protein transport as a function of oligomeric state.
• 4:30 PM – Markita Landry - Imaging Neuromodulation in the Brain with Near-Infrared Fluorescent Nanosensors.
• 5:00 PM – Weber Thesis Award Winner. Lorenzo Scipioni: High-content determination of the cell state by Phasor S-FLIM
• 5:30 PM – Weber Lecturer. Alberto Diaspro: The multimodal microscope and the Plato’s allegory of the cave. Increasing knowledge by coupling fluorescence and label-free mechanisms of contrast.

Wednesday, January 11, 2023

• 7:30 AM – Breakfast at dinning room, followed posters, and exhibits.
• Confirmed short talks
• 9:00 AM – Christian Combs: Convolutional Neural Network Transformer (CNNT) for Fluorescence Microscopy Denoising with Improved Generalization and Fast Adaption.
• 9:15 AM – Florencia Irigoín: Study of biophysical properties of the cilium that regulate protein transport to the organelle.
• 9:30 AM – Rajesh Ramachandran: The predicted coiled-coil motif of intrinsically disordered mitochondrial Fission Factor (Mff) functions as a lipid-sensing amphipathic helix.
• 9:45 AM – Irene Carlon-Andres: Quantitative characterization of HIV-1 viruses using fluorescence lifetime correlation spectroscopy: Lifetime-based Virometry.
• 10:00 AM – Camila Oses: The dynamical organization of pluripotency transcription factors respond to differentiation cues in early S-phase.
• 10:15 AM – TBD.
• 10:30 AM – Elaine Petroniho: Can Heparin Modulate Prion Protein Liquid-Liquid Phase Separation?.
• 10:45 AM – Rest of the day free.

Thursday, January 12, 2023

• 7:30 AM – Breakfast at dinning room, followed posters, and exhibits.
• Chair: Francisco Barrantes
• 9:00 AM – Francesco Palomba: Advanced fluorescence microscopy techniques for the biophysical understanding of protein condensates.
• 9:30 AM – Jerson Silva: The chameleonic behavior of p53 in health and disease: Phase separation and aggregation of mutant p53 as an emerging target in cancer.
• 10:00 AM – Melissa Birol: Imaging disordered protein systems through different scales.
• 10:30 AM – Break, posters and exhibits.
• Chair: Debora Foguel
• 11:15 AM – Joachim Mueller: Partitioning of ribonucleoprotein complexes from the cellular actin cortex.
• 11:45 AM – Thorsten Wohland: Computational Advances and Applications in Imaging Fluorescence Correlation Spectroscopy.
• 12:15 PM – Luciano Masullo: Single-molecule localization with molecular-scale precision by raster scanning a minimum of light.
• 12:45 PM – Lunch.
• Luis Bagatolli Memorial Session
• Chair: Enrico Gratton
• 2:15 PM – Leonel Malacrida: Accessing to cellular innards water dipolar relaxation by dimethylamino-naphthalene probes and phasor analysis of multiplexing fluorescence microscopy.
• 2:45 PM – Susana Sanchez: Cell Membrane heterogeneity and media stiffness.
• 3:15 PM – David Jameson: Nanoimaging Studies on Cytoplasmic and Nuclear Arc/Arg3.1.
• 3:45 PM – Soledad Celej: Exploring the phase behavior of the amyloidogenic proteins alpha-synuclein and Tau.
• 4:15 PM – Break, posters and exhibits.
• 7:00 PM – Symposium Banquet.

Friday, January 13, 2023

• 7:30 AM – Breakfast at dinning room, followed posters, and exhibits.
• Chair: Thorsten Wohland
• 9:00 AM – Michelle Digman: The phasor approach to FLIM for Metabolic profiling and Mitometer for tracking phenotypic changes in mitochondria in cancer cells.
• 9:30 AM – Debora Foguel: The role of neutrophils and neutrophil extracellular traps (NETs) in amyloid diseases.
• 10:00 AM – Vicente Parot: All-optical neurophysiology using high-speed wide-area optical sectioning.
• 10:30 AM – Break, posters and exhibits.
• Chair: David Jameson
• 11:15 AM – Taekjip Ha: Light, CRISPR and DNA repair.
• 11:45 AM – Elliot Elson: Lipid Domain Growth Studied by iFCS.
• 12:30 PM – Closing remarks and acknowledgements.Leonel Malacrida and Enrico Gratton.
• 12:45 PM – Lunch.