Laboratory for Fluorescence Dynamics

A national research center for biomedical fluorescence spectroscopy at the University of California, Irvine



We gratefully acknowledge the sponsorship of the following organizations:

ISS Inc.


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    LFD Workshop Agenda

18th LFD Workshop in Advanced Fluorescence Imaging and Dynamics

October 21–24, 2024 iCalendar

Laboratory for Fluorescence Dynamics
University of California, Irvine
Department of Biomedical Engineering
3208 Natural Sciences II
Irvine, CA, 92697-2715, USA

Dates and Updates

  • October 21, 2024: Workshop starts at 9:00 AM PST.
  • August 231, 2024: Deadline for submitting UCI student early registration.
  • April 10, 2024: Online registration opened.
  • March 13, 2024: Online registration will open soon


Once a year the Laboratory for Fluorescence Dynamics (LFD) organizes an advanced workshop, which outlines the advanced concepts of fluorescence imaging techniques and instrumentation.

The workshop is designed for students who use fluorescence microscopy instrumentation and techniques, as well as for researchers and industrial scientists who intend to extend their knowledge of these fields.

The workshop consists of:

Topics on the agenda:

  • Single Point and Scanning Fluorescence Correlation Spectroscopy (FCS)
  • Raster Image Correlation Spectroscopy (RICS)
  • Fluorescence Lifetime Imaging (FLIM) and Förster Resonance Energy Transfer (FRET)
  • Number and Brightness (N&B)
  • Pair Correlation Function (pCF)
  • Spectral Phasors
  • Particle Tracking (PT) in three dimensions
  • Deep tissue imaging, DIVER
  • Single plane illumination microscopy (SPIM)
  • Image Mean Square Displacement (iMSD)
  • Data analysis and simulations using Globals for Images · SimFCS software
  • Featuring bioluminescent tools for real-time imaging of immunometabolism

The main lecture presentations are recorded (audio and video) and published on this website.

For details about the topics refer to the lecture recordings and notes of the previous workshops in 2023, 2022, 2021, 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011, 2010, 2009, 2008, 2007, and 2006.

Tentative Agenda



It is recommended that participants have at least a bachelor's degree in the life sciences, physical sciences or engineering before attending this course.

Registration and Payment

Registration fees are:

  • $1000 for industry/private researchers
  • $650 for academia/university researchers
  • $350 for students

The registration and payment process is:

  1. Submit the online registration form and print the confirmation page for your records. Please note that this page merely confirms that we have received your registration information; it is not a confirmation of participation in the workshop or the laboratory training.
  2. Pay your registration fees by credit card using the UC Irvine Marketplace LFD Workshop store.
  3. Once we receive your online registration and payment, your participation is reserved. We will send an email confirming your participation in the workshop and laboratory training (if applicable) by October 6, 2024.


  • Platinum: $4000.
  • Gold: $3000.
  • Silver: $2000.

Contact Information

Contact the LFD for questions and comments regarding the 18th LFD Workshop by email