Laboratory for Fluorescence Dynamics

A national research center for biomedical fluorescence spectroscopy at the University of California, Irvine


LFD Workshop Flyer


We gratefully acknowledge the sponsorship of the following organizations:




Carl Zeiss Inc. Logo

Leica Microsystems



ISS Inc.

Globals Software

University of California, Irvine


17th LFD Workshop in Advanced Fluorescence Imaging and Dynamics

October 23–27, 2023 iCalendar

Laboratory for Fluorescence Dynamics
University of California, Irvine
Department of Biomedical Engineering
3208 Natural Sciences II
Irvine, CA, 92697-2715, USA

Dates and Updates

  • October 23, 2023: Workshop starts at 9:00 AM PST.
  • October 13, 2023: Online registration closes.
  • October 5, 2023: Financial assistance application closed.
  • March 13, 2023: Online registration opened.


Once a year the Laboratory for Fluorescence Dynamics (LFD) organizes an advanced workshop, which outlines the advanced concepts of fluorescence imaging techniques and instrumentation.

The workshop is designed for students who use fluorescence microscopy instrumentation and techniques, as well as for researchers and industrial scientists who intend to extend their knowledge of these fields.

The workshop consists of:

Topics on the agenda:

  • Single Point and Scanning Fluorescence Correlation Spectroscopy (FCS)
  • Raster Image Correlation Spectroscopy (RICS)
  • Fluorescence Lifetime Imaging (FLIM) and Förster Resonance Energy Transfer (FRET)
  • Number and Brightness (N&B)
  • Pair Correlation Function (pCF)
  • Spectral Phasors
  • Particle Tracking (PT) in three dimensions
  • Deep tissue imaging, DIVER
  • Single plane illumination microscopy (SPIM)
  • Image Mean Square Displacement (iMSD)
  • Data analysis and simulations using Globals for Images · SimFCS software
  • Featuring bioluminescent tools for real-time imaging of immunometabolism

The main lecture presentations are recorded (audio and video) and published on this website.

For details about the topics refer to the lecture recordings and notes of the previous workshops in 2022, 2021, 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011, 2010, 2009, 2008, 2007, and 2006.


It is recommended that participants have at least a bachelor's degree in the life sciences, physical sciences or engineering before attending this course.

A personal computer with Microsoft Windows 10 operating system is required for running the data analysis and simulation software, Globals for Images · SimFCS 2, during the computer-based training. Minimum system requirements are Intel Core 2 CPU, 4 GB RAM, 1 free USB port, Windows 10 Pro operating system or compatible. Windows 8, Home and Tablet editions are not supported. Administrator rights are required for installing the software. A PDF reader software is required for viewing electronic lecture notes and tutorials.

Registration and Payment

Registration fees are:

  • $1000 for industry/private researchers
  • $650 for academia/university researchers
  • $350 for students

The registration and payment process is:

  1. Submit the online registration form and print the confirmation page for your records. Please note that this page merely confirms that we have received your registration information; it is not a confirmation of participation in the workshop or the laboratory training.
  2. Pay your registration fees by credit card using the UC Irvine Marketplace LFD Workshop store.
  3. Once we receive your online registration and payment, your participation is reserved. We will send an email confirming your participation in the workshop and laboratory training (if applicable) by October 6, 2023.


  • Platinum: $5000.
  • Gold: $3000.
  • Silver: $2000.
  • Tabletop: $1000.

Contact Information

Contact the LFD front desk for questions and comments regarding the 17th LFD Workshop by email