Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine


LFD Workshop Flyer


We gratefully acknowledge the sponsorship of the following organizations:

UCI Skin Logo

ISS Inc.

Leica Microsystems

Globals Software

University of California, Irvine

14th LFD Workshop in Advanced Fluorescence Imaging and Dynamics

October 21–25, 2019 iCalendar

Laboratory for Fluorescence Dynamics
University of California, Irvine
Department of Biomedical Engineering
3208 Natural Sciences II
Irvine, CA, 92697-2715, USA

Dates and Updates


Once a year the Laboratory for Fluorescence Dynamics (LFD) organizes an advanced workshop, which outlines the advanced concepts of fluorescence imaging techniques and instrumentation.

The workshop consists of:

The workshop is designed for students who use fluorescence microscopy instrumentation and techniques, as well as for researchers and industrial scientists who intend to extend their knowledge of these fields.

Topics on the agenda:

  • Single Point and Scanning Fluorescence Correlation Spectroscopy (FCS)
  • Raster Image Correlation Spectroscopy (RICS)
  • Fluorescence Lifetime Imaging (FLIM) and Förster Resonance Energy Transfer (FRET)
  • Number and Brightness (N&B)
  • Pair Correlation Function (pCF)
  • Spectral Phasors
  • Particle Tracking (PT) in three dimensions
  • Deep tissue imaging, DIVER
  • Single plane illumination microscopy (SPIM)
  • Image Mean Square Displacement (iMSD)
  • Data analysis and simulations using Globals for Images · SimFCS software

For details about the topics refer to the lecture recordings and notes of the previous workshops in 2019, 2018, 2017, 2016, 2015, 2014, 2013, 2012, 2011, 2010, 2009, 2008, 2007, and 2006.


The laboratory training is limited to 20 participants, whereas enrollment in the lectures and the computer based training is open.

It is recommended that participants have at least a bachelor's degree in the life sciences, physical sciences or engineering before attending this course.

A personal notebook computer with Microsoft Windows operating system is required for running the data analysis and simulation software, Globals for Images · SimFCS 2, during the computer based training. Minimum system requirements are: Intel Core 2 CPU, 4 GB RAM, 1 free USB port, Windows 7 Pro operating system or compatible. Windows XP, Home and Tablet editions are not supported. Administrator rights are required for installing the software. A PDF reader software is required for viewing electronic lecture notes and tutorials.

The main lecture presentations will be recorded (audio and video) and published on this website.

Registration and Payment

Registration fees are:

  • $350 for students
  • $650 for academia/university researchers
  • $1100 for industry/private researchers

The fees include meals (breakfast, lunch and dinner).

The registration and payment process is:

  1. Submit the online registration form and print the confirmation page for your records. Please note that this page merely confirms that we have received your registration information; it is not a confirmation of participation in the workshop or the laboratory training.
  2. Pay your registration fees:
    • by credit card using the UC Irvine Marketplace LFD Workshop store,
    • or by mailing a check, payable to the "UC Regents" and referencing the "LFD Workshop", to the following address. We cannot process payment by bank transfer.
LFD Workshop 2019
c/o Enrico Gratton
University of California, Irvine
Biomedical Engineering Department
3208 Natural Sciences II
Irvine, CA, 92697-2715
  1. Once we receive your online registration and payment, your participation is reserved. We will send an email confirming your participation in the workshop and laboratory training (if applicable) by September 23, 2019.

Registration Awards for Students

There will be up to 6 registration waivers for students. The winners will have the opportunity to present their work as a poster during the workshop. Please submit a short abstract of your work (half page) and a support letter from your advisor before September 20, 2019, to:

LFD Workshop 2019
c/o Enrico Gratton
University of California, Irvine
Biomedical Engineering Department
3208 Natural Sciences II
Irvine, CA, 92697-2715

Accommodation and Directions

As in the previous years, we will try to negotiate special rates with a hotel near the Orange County airport.

Refer to the visitors guide for other lodging options and directions to the LFD.


The LFD workshop relies on the generosity of sponsors.

We offer several levels of sponsorship:

  • Gold Sponsorship: $2000 or more
    Gold sponsors receive two registration passes and may present a 30 minute scientific talk at the workshop.
  • Silver Sponsorship: $1500
    Silver sponsors recieve one registration pass and may present a 20 minute scientific talk at the workshop.
  • Table-top Sponsorship: $500
    Table-top sponsors will be given a table to exhibit marketing materials. Registration for the workshop is not included.

These options are not exhaustive. Please contact us to sponsor the student waiver program, demonstrate an instrument, provide USB drives, or to pay for the banquet, lunch or dinner.

Contact Information

Contact the LFD front desk for questions and comments regarding the 17th LFD Workshop:

Enrico Gratton
Phone: +1‑949‑824‑7085