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Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- kICS: k-space Image Correlation Spectroscopy
- N&B: Number and Brightness
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- RICS: Raster Image Correlation Spectroscopy
- STICS: Spation-Temporal Image Correlation Spectroscopy
- more...
1st LFD Workshop (2006)
The "1st LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 23–26, 2006, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Agenda and Downloads
Lecture notes are for personal use only and may not be redistributed without the authors permission.
Monday, October 23, 2006
- Breakfast
- 09:00-09:45 - Lecture 1. Enrico Gratton: Introduction to advanced FCS
- 09:45-10:30 - Lecture 2. Jay Unruh: PCH and PSF
- Break
- 11:00-11:45 - Lecture 3. Theodore Hazlett: FCS applications
- 11:45-12:30 - Lecture 4. Enrico Gratton: Introduction to spatio temporal scanning FCS
- 12:30-12:40 - Fellowship talk 1. Alexandra Albanese: Fast determination of dissociation constants of PI binding proteins using FCS.
- Lunch
- 13:30-15:00 - Training on FCS. Download Laboratory and computing tutorials.
- Break
- 15:30-17:00 - Training continued.
- 17:00-17:10 - Fellowship talk 2. Ellen Gielen: Probing diffusion laws within cellular membranes by one-photon Z-scan FCS.
- 17:10-17:20 - Fellowship talk 3. Travis Moore: Mating response of S. cerevisiaein microfluidically generated mating factor gradients.
- 17:20-18:00 - Social Event.
Tuesday, October 24, 2006
- Breakfast
- 09:00-09:45 - Lecture 5. Enrico Gratton: Image correlation methods for the internal dynamics of cells
- 09:45-10:30 - Lecture 6. Enrico Gratton: Introduction to RICS
- Break
- 11:00-11:45 - Lecture 7. Michelle Digman: RICS
- 11:45-12:30 - Lecture 8. Michelle Digman: RICS2 - Applications tools for microscopy
- 12:30-12:40 - Fellowship talk 4. Asmahan AbuArish: Origins of the nuclear localization of the protein Ran.
- Lunch
- 13:30-15:00 - Training on RICS. Download Laboratory and computing tutorials, RICS data files
- Break
- 15:30-17:00 - Training continued.
- 17:00-17:20 - Invited talk. Yiwei Jia from Olympus.
- 17:20-18:00 - Social Event.
Wednesday, October 25, 2006
- Breakfast
- 09:00-09:45 - Lecture 9. Enrico Gratton: RICS3 - Number of particles and brightness by the moment analysis
- 09:45-10:30 - Lecture 10. Enrico Gratton: FLIM using the Phasor approach - Application to FRET analysis
- Break
- 11:00-11:45 - Lecture 11. Susana Sanchez: Calcium concentration in ex-vivo skin Phasor representation
- 11:45-12:30 - Lecture 12. Michelle Digman: FRET applications
- 12:30-12:40 - Fellowship talk 5. Kirsten Altenbach: Development and analysis of recombinant calcium sensors for use in live cell imaging of filamentous fungi.
- Lunch
- 13:30-15:00 - Training on FLIM and FRET. Download Laboratory and computing tutorials, FLIM data files
- Break
- 15:30-17:00 - Training continued.
- Break
- 17:20-18:00 - Special lecture. David Jameson: A Nano-History of Fluorescence
- 18:00-20:00 - Banquet.
Thursday, October 26, 2006
- Breakfast
- 09:00-09:45 - Lecture 13. Oliver Holub: Advanced instrumentation
- 09:45-10:30 - Lecture 14. Claudia Lee: Hardware for FLIM - the FLIMBox
- Break
- 11:00-11:10 - Fellowship talk 6. Christopher B. Raub: Collagen gel microstructure correlates with second harmonic generation and mechanical properties.
- 11:10-12:40 - Discussion and Closure.
Lecturers
- Ryan A Colyer
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Theodore L Hazlett
Laboratory for Fluorescence Dynamics, University of California, Irvine.
- Oliver Holub
Laboratory for Fluorescence Dynamics, University of California, Irvine. - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa. - Claudia Y Lee
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Susana A Sanchez
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Jay R Unruh
Laboratory for Fluorescence Dynamics, University of California, Irvine.
Award Winners
Six registration waivers, sponsored by the Cell Migration Consortium and the Beckman Laser Institute, have been awarded to the following students:
- Asmahan AbuArish
McMaster University, Physics & Astronomy, Hamilton, Ontario, Canada. - Alexandra Albanese
University of Illinois at Chicago, Chemistry, Chicago, IL. - Kirsten Altenbach
University of Edinburgh, School of Chemistry, Edinburgh, United Kingdom. - Ellen Gielen
Katholieke Universiteit Leuven, Biomoleculaire Dynamica, Leuven, Belgium. - Travis Moore
University of California, Irvine, Developmental and Cellular Biology, Irvine, CA. - Christopher B Raub
University of California, Irvine, Biomedical Engineering, Irvine, CA .
Paid Attendees
- Nicholas Barry
University of Colorado Health Sciences Center, Renal Division C281, Denver, CO. - Jennifer Barton
The University of Arizona, Biomedical Engineering, Tucson, AZ. - Christian Combs
National Institutes of Health, NHLBI, Bethesda, MD. - Ester Fernandez-Salas
Allergan Inc, Biological Sciences, Irvine, CA. - Cecile Fradin
McMaster University, Physics and Astronomy, Hamilton, Canada. - Cardarelli Francesco
Scuola Normale Superiore, Laboratorio di Fisica, NEST-CNR, Pisa, Italy. - Patton E Garay
Allergan Inc, Biological Sciences, Irvine, CA. - Prabhakar Gudla
SAIC/NCI-Frederick, Image Analysis, Frederick, MD. - Frank Hecht
Carl Zeiss MicroImaging GmbH, AIM, Jena, Germany. - Joseph Huff
Stowers Institute for Medical Research, Advanced Instrumentation and Physics, Kansas City, MO. - Guohua Lei
The University of Illinois at Chicago, Department of Chemistry, Chicago, IL. - Moshe Levi
University of Colorado Health Sciences Center, Medicine, Physiology and Biophysics, Denver, CO. - Yuting Lin
University of California, Irvine, Center for Functional Onco Imaging, Irvine, CA. - Nzola De Magalhaes
University of California, Irvine, Biomedical Engineering, Irvine, CA. - Matilde Marchi
Scuola Normale Superiore, Neuroscience Institute CNR, Pisa, Italy. - William Glenn McGregor
University of Louisville, Pharmacology and Toxicology, Louisville, KY. - Giampiero Mei
University of Rome Tor Vergata, Experimental Medicine and Biochemistry, Rome, Italy. - Hirdyesh Mishra
Indian Insititute of Science, Molecular Biophysics Unit, Bangalore, India. - Ralf Netz
Carl Zeiss MicroImaging GmbH, Advanced Imaging Microscopy, Jena, Germany. - Bizzarri Ranieri
Scuola Normale Superiore, Laboratorio di Fisica, NEST-CNR, Pisa, Italy. - Tilman Rosales
National Institutes of Health, NHLBI, Laboratory of Molecular Biophysics, Bethesda, MD. - Hugo Sanabria
University of Texas Health Science Center at Houston, Neurobiology and Anatomy, Houston, TX. - Joel Schwartz
Stowers Institute for Medical Research, Imaging, Kansas City, Missouri. - Debasish Sen
University of California, Irvine, Physiology and Biophysics, Irvine, California. - Brian Slaughter
Stowers Institute for Medical Research, Imaging, Kansas City, MO. - Aleksandr Smirnov
National Institutes of Health, NHLBI, Bethesda, USA. - Nick Smisdom
University Hasselt, Cell Physiology, Diepenbeek, Belgium. - Lorenzo Stella
University of Rome Tor Vergata, Department of Chemical Sciences and Technologies, Roma, Italy. - Shuo Tang
University of California, Irvine, Beckman Laser Institute, Irvine, CA. - Shang-You Tee
University of Washington, Center for Cell Dynamics, Department of Biology, Friday Harbor, WA. - Satoru Uzawa
University of California, Berkeley, HHMI, Molecular & Cell Biology, Berkeley, CA. - Jo Vercammen
Katholieke Universiteit Leuven, Biomolecular Dynamics, Leuven, Belgium. - Jerome Webber
University of California, Irvine, Molecular Biology & Biochemistry, Irvine, CA. - Leor Weinberger
Princeton University, Dept. of Molecular Biology, Princeton, NJ. - Klaus Weisshart
Carl Zeiss MicroImaging GmbH, Advanced Imaging Microscopy, Jena, Germany. - Dudley Williams
Allergan Inc, NTP Gp. Biology, Irvine, CA.