Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Group Photo
Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- kICS: k-space Image Correlation Spectroscopy
- N&B: Number and Brightness
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- STICS: Spation-Temporal Image Correlation Spectroscopy
- more...
2nd LFD Workshop (2007)
The "2nd LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 22–26, 2007, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 22, 2007
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to advanced FCS
- 09:45-10:30 – Lecture 2. Jay Unruh: The photon counting histogram
- Break
- 11:00-11:45 – Lecture 3. Enrico Gratton: Introduction to spatio temporal scanning FCS
- 11:45-12:15 – Application 1. Susana Sánchez: Applications of FCS
- 12:15-12:45 – Fellowship talks
- Shashank Bharill: Dissecting the immune response in asthma using fluorescence technology
- Sergi Padilla: Using fluorescence microscopy to understand cytoskeleton dynamics in living cells
- Isabell Pechtl: Fluorescence resonance energy transfer as a tool for structural investigation of macromolecules
- Lunch
- 13:30-15:00 – Computer based training on FCS. Download Computing tutorials and data files
- Break
- 15:30-17:20 – Computer based training on FCS continued.
- 17:20-18:00 – Social event.
Tuesday, October 23, 2007
- Breakfast
- 09:00-09:45 – Lecture 4. Enrico Gratton: Introduction to ICS
- 09:45-10:30 – Lecture 5. Paul Wiseman: STICS and kICS
- Break
- 11:00-11:45 – Lecture 6. Michelle Digman: The RICS method
- 11:45-12:15 – Application 2. Michelle Digman: Applications of RICS
- 12:15-12:45 – Fellowship talks
- Pabak Sarkar: Photophysical study of Cerulean and Venus fluorescent proteins
- Nick Watson: Intracellular dynamics of proteins required for the resolution of DNA replication forks blocked by genotoxic carcinogens
- Leor Weinberger: Gene expression noise correlation spectroscopy to quantify transcriptional processes in live cells
- Lunch
- 13:30-15:00 – Computer based training on RICS. Download Computing tutorials and data files
- Break
- 15:30-17:20 – Computer based training on RICS continued.
- 17:20-18:00 – Special lecture 1. Robert M Clegg: The history of FRET
- 18:00-20:00 – Social event.
Wednesday, October 24, 2007
- Breakfast
- 09:00-09:45 – Lecture 7. Enrico Gratton: N&B analysis
- 09:45-10:30 – Lecture 8. Enrico Gratton: The phasor approach to FLIM and FRET
- Break
- 11:00-11:45 – Lecture 9. Ryan Colyer: Digital frequency domain FLIM
- 11:45-12:15 – Application 3. Valeria Caiolfa: Applications of the FLIM phasor
- 12:15-12:45 – Application 4. Asmahan Abu-Arish: Applications of N&B
- Lunch
- 13:30-15:00 – Computer based training on FLIM. Download Computing tutorials and data files
- Break
- 15:30-16:40 – Computer based training on FLIM continued.
- 17:00-18:00 – Special lecture 2. David M Jameson: A nano-history of fluorescence
- 18:00-20:00 – Banquet.
Thursday, October 25, 2007
- Breakfast
- 09:00-09:45 – Lecture 10. Oliver Holub: Advanced instrumentation
- 09:45-10:30 – Lecture 11. Christian Hellriegel: Particle tracking
- Break
- 11:00-12:45 – Discussion.
- Lunch
- 13:30-15:00 – Laboratory training. Download Laboratory tutorials
- Break
- 15:30-17:20 – Laboratory training continued.
- 17:20-18:00 – Social event.
Friday, October 26, 2007
- Breakfast
- 09:00-10:30 – Laboratory training. Download Laboratory tutorials
- Break
- 11:00-12:45 – Laboratory training continued.
- Lunch
- 13:30 – Closure
Lecturers
- Asmahan Abu-Arish
Department of Physics and Astronomy, McMasters University, Canada. - Valeria R Caiolfa
Fondazione Centro San Raffaele, Scientific Institute, Milan, Italy. - Robert M Clegg
Department of Physics, University of Illinois at Urbana-Champaign. - Ryan A Colyer
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Christian Hellriegel
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Oliver Holub
Laboratory for Fluorescence Dynamics, University of California, Irvine. - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Susana A Sánchez
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Jay R Unruh
Laboratory for Fluorescence Dynamics, University of California, Irvine. - Paul W Wiseman
Department of Physics and Chemistry, McGill University, Montreal, Canada.
Award Winners
Six registration waivers, sponsored by the Cell Migration Consortium and ISS, Inc. (Champaign, IL), have been awarded to the following students:
- Sohashank Bharill
University of North Texas Health Science Center, Biochemistry and Molecular Biology, Fort Worth, TX. - Sergi Padilla
Institute Jacques Monod, Cellular Biology, Paris, France. - Isabell Pechtl
University of Edinburgh, Chemistry, Edinburgh, UK. - Pabak Sarkar
University of North Texas Health Science Center, Molecular Biology and Immunology, Fort Worth, TX. - Nick Watson
University of Louisville, Pharmacology and Toxicology, Louisville, KY. - Leor Weinberger
Princeton University, Molecular Biology, Princeton, NJ.
Paid Attendees
- Lorenzo Albertazzi
Scuola Normale Superiore, Biophysics, Pisa, Italy. - Rachel Williams Bailey
Brigham Young University, Physiology and Developmental Biology, Provo, UT. - John D Bell
Brigham Young University, Physiology and Developmental Biology, Provo, UT. - Jonathan Brewer
University of Southern Denmark, BMB/MEMPHYS, Odense, Denmark. - Ron S Broide
Allergan Inc, Biological Science, Irvine, CA. - Christof Buehler
Novartis Institutes for BioMedical Research, Discovery Technologies - Innovative Screening Technologies, Vienna, Austria. - Brian Cai
Allergan Inc, Biological Sciences, RD3-3B, Irvine, CA. - Andrea Callegari
Scuola Normale Superiore, Biophysics, Pisa, Italy. - Marcella Gilmore
Allergan Inc, Biological Sciences, RD3-3B, Irvine, CA. - Ravi Jasuja
Boston University School of Medicine, Physiology and Biophysics, Boston, USA. - David A Johnson
University of California, Riverside, Biomedical Sciences, Riverside, CA. - Donald C Lamb
Ludwig-Maximilians-Universität München, Physical Chemistry, Munich, Germany. - Park Lee
University of Illinois at Chicago, Chemistry, Chicago, IL. - Zhiwei Li
Allergan Inc, Biological Science, Irvine, CA. - Nikita Malavia
University of California, Irvine, Biomedical Engineering, Irvine, CA. - Kaustav Nandy
National Cancer Institute / SAIC - Frederick, Image Analysis Lab, Frederick, MD. - Jennifer Nelson
Brigham Young University, Physiology and Developmental Biology, Provo, UT. - Erin D Olson
Brigham Young University, Physiology and Developmental Biology, Provo, UT. - Rajesh Ramachandran
The Scripps Research Institute, Cell Biology, La Jolla, CA. - Nina K Reitan
Norwegian University of Science and Technology, Physics, Trondheim, Norway. - Justin Ross
University of Hawaii, Cell and Molecular Biology, Honolulu, HI. - Kenneth E Sawin
University of Edinburgh, Wellcome Trust Centre for Cell Biology, Edinburgh, United Kingdom. - Parijat Sengupta
Stony Brook University Medical Center, Physiology and Biophysics, Stony Brook, NY. - Malathi Srivatsan
Arkansas State University, Biological Sciences/ABI, Jonesboro, AR. - Nicole Wakida
University of California, Irvine, Developmental and Cell Biology, CA. - Robert Walder
University of California, Irvine, Physics and Astronomy, Irvine, CA. - Mark Washburn
Allergan Inc, Biological Sciences, RD3-3B, Irvine, CA. - Janice Yoshizawa
University of California, Irvine, Molecular Biology and Biochemistry, Irvine, CA. - Tiefeng You
ISS Inc., Champaign, IL. - Moreno Zamai
Scientific Institute, Fondazione Centro San Raffaele, Milan, Italy. - Shaohua Zhou
University of California, Irvine, Developmental and Cell Biology, Irvine, CA.