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Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
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Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- FSM: Fluorescence Speckle Microscopy
- ICS: Image Correlation Spectroscopy
- kICS: k-space Image Correlation Spectroscopy
- N&B: Number and Brightness
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- STICS: Spation-Temporal Image Correlation Spectroscopy
- more...
3rd LFD Workshop (2008)
The "3rd LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 27–31, 2008, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 27, 2008
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 11:45-12:15 – Application 1. Susana Sánchez: Applications of FCS.
- 12:15-12:45 – Fellowship talks
- Melissa Wong: The dynamics of the Human Cytomegalovirus (HCMV) Major Immediate-Early (MIE) circuit and implications for latency.
- Agnes Foeglein: Using FRAP to investigate host range interaction of influenza A virus.
- Nicholas Grant James: Dissecting the intrinsic tryptophan quenching events of human serum transferrin during iron binding and release.
- Lunch
- 14:00-17:30 – Computer based training on FCS. Download computing tutorials and data files
- 17:30-18:00 – Social event.
Tuesday, October 28, 2008
- Breakfast
- 09:00-09:45 – Lecture 4. Michelle Digman: The number and brightness (N&B) method
- 09:45-10:30 – Lecture 5. Enrico Gratton: Introduction to spatio-temporal scanning FCS (sFCS)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: Image correlation spectroscopy (ICS) methods
- 11:45-12:15 – Application 2. Giulia Ossato: Applications of N&B.
- 12:15-12:45 – Fellowship talks
- Kaushik Gurunathan: Understanding nucleosome dynamics using correlation analysis.
- Shan Tao: Role of protein self-association in the membrane-deforming activity of ENTH domain.
- Neil Anthony: Intracellular dynamics and interactions of nuclear import receptor proteins by sFCS and RICS.
- Lunch
- 14:00-17:30 – Computer based training on RICS. Download computing tutorials and data files
- 17:30-18:00 – Special lecture 1. David M Jameson: A nano-history of fluorescence
- 18:00-20:00 – Banquet.
Wednesday, October 29, 2008
- Breakfast
- 09:00-09:45 – Lecture 7. Gaudenz Danuser: Fluorescence speckle microscopy (FSM)
- 09:45-10:30 – Lecture 8. Klaus Hahn: Biosensor designs
- Break
- 11:00-11:45 – Lecture 9. Klaus Hahn: Practical aspects of biosensors
- 11:45-12:15 – Application 3. Gaudenz Danuser: Computational multiplexing.
- 12:15-12:45 – Application 4. Klaus Hahn: Biosensor applications.
- Lunch
- 14:00-17:30 – Computer based training on biosensors. Download biosensor computing tutorials and data files
- 17:30-18:00 – Special lecture 2. Valeria Caiolfa: Living colors
- 18:00-18:30 – Social event.
Thursday, October 30, 2008
- Breakfast
- 09:00-09:45 – Lecture 10. Oliver Holub: Advanced instrumentation
- 09:45-10:30 – Lecture 11. Enrico Gratton: The phasor approach to FLIM and FRET
- Break
- 11:00-11:45 – Lecture 12. Christian Hellriegel: Particle tracking
- 11:45-12:15 – Application 4. Moreno Zamai: Applications of FLIM.
- 12:15-12:45 – Discussion.
- Lunch
- 14:00-17:30 – Laboratory training on FCS. Download laboratory tutorials
- 17:30-18:00 – Social event.
Friday, October 31, 2008
- Breakfast
- 09:00-12:45 – Laboratory training on RICS and FLIM. Download laboratory tutorials
- Break
- 12:45 – Closure
Lecturers
- Valeria R Caiolfa
Fondazione Centro San Raffaele, Scientific Institute, Milan, Italy and Microscopy Unit, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain - Gaudenz Danuser
Laboratory for Computational Cell Biology, The Scripps Research Institute, La Jolla, USA - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Klaus Hahn
Department of Pharmacology, University of North Carolina at Chapel Hill, USA - Christian Hellriegel
Microscopy Unit, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain - Louis Hodgson
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, New York, USA - Oliver Holub
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Giulia Ossato
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Susana A Sánchez
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Moreno Zamai
Fondazione Centro San Raffaele, Scientific Institute, Milan, Italy
Award Winners
Six registration waivers, sponsored by the Cell Migration Consortium (CMC), have been awarded to the following students:
- Neil Anthony
Physics, Emory University, Atlanta, GA. - Agnes Foeglein
Virology, University of Cambridge, Cambridge, United Kingdom. - Kaushik Gurunathan
Chemistry and Biochemistry, Arizona State University, Tempe, AZ. - Nicholas Grant James
Biochemistry, University of Vermont, Burlington, VT. - Shan Tao
Chemistry, University of Illinois at Chicago, Chicago, IL. - Melissa Wong
Chemistry and Biochemistry, University of California, San Diego, San Diego, CA.
Paid Attendees
- Salim Abdisalaam
Bioengineering, University of Texas at Arlington, Arlington, TX. - Luis Bagatolli
MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, Odense M, Denmark. - Caroline Clerte
Centre de Biochimie Structurale, Montpellier, France. - Samuel Connell
Cell and Tissue Imaging Center, St. Jude Children's Research Hospital, Memphis, TN. - Fabio Formiggini
Dynamic Imaging Microscopy, CEINGE biotecnologie avanzate Scarl, Naples, Italy. - Marcella Gilmore
Allergan Inc, Irvine, CA. - Andre Marco Oliveira Gomes
Medical Biochemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. - Chyong Jy
Ophthalmology, University of California, Irvine, Orange, CA. - Syed J Khundmiri
Renal Medicine, University of Louisville, Louisville, KY. - Jianwei Li
Allergan Inc, Irvine, CA. - Robert Mahen
Oncology, Hutchison/MRC Research Centre, University of Cambridge, Cambridge, United Kingdom. - Will Marshall
Imaging, Stowers Institute for Medical Research, Kansas City, MO. - Andrea Cheble de Oliveira
Medical Biochemistry, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil. - Kurt Short
Chemistry, New Mexico Highlands University, Las Vegas, NM. - Yong Wang
Chemistry, University of California, Irvine, Irvine, CA. - Dudley Williams
Allergan Inc, Irvine, CA. - Guikai Wu
Biological Chemistry, University of California, Irvine, Irvine, CA.