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Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
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Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- FSM: Fluorescence Speckle Microscopy
- ICS: Image Correlation Spectroscopy
- kICS: k-space Image Correlation Spectroscopy
- N&B: Number and Brightness
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- STICS: Spation-Temporal Image Correlation Spectroscopy
- more...
4th LFD Workshop (2009)
The "4th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 26–30, 2009, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 26, 2009
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Susana Sánchez: Introduction to scanning FCS (sFCS)
- 11:45-12:15 – Application 1. Pierre Moens: Single point FCS and N&B on a commercial confocal scanning microscope with analog detector.
- 12:15-12:45 – Fellowship talks
- Julia Philip: RICS applied to microtubule dynamics.
- Emmanuel Adu-Gyamfi: Targeting Ebola virus at the plasma membrane.
- Lunch
- 14:00-17:30 – Computer based training on FCS. Download computing tutorials and data files
- 17:30-18:00 – Social event.
Tuesday, October 27, 2009
- Breakfast
- 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Michelle Digman: The number and brightness method (N&B)
- Break
- 11:00-11:45 – Lecture 6. Paul Wiseman: STICS and kICS
- 11:45-12:15 – Application 2. Giulia Ossato: Huntingtin (Htt) oligomer formation in live cells.
- 12:15-12:45 – Application 3. David Jameson: A new twist on Dynamin.
- Lunch
- 14:00-17:30 – Computer based training on RICS. Download computing tutorials and data files
- 17:30-18:00 – Special lecture 1. David Jameson: A nano-history of fluorescence
- 18:00-20:00 – Social event.
Wednesday, October 28, 2009
- Breakfast
- 09:00-09:45 – Lecture 7. Klaus Hahn: Biosensors and computational tools to study signaling networks in living cells
- 09:45-10:30 – Lecture 8. Klaus Hahn: Recent developments: protein manipulation in vivo.
- Break
- 11:00-11:45 – Lecture 9. Louis Hodgson: Biosensor image processing
- 11:45-12:15 – Lecture 10. Michelle Digman: Cross-RICS and cross-N&B
- 12:15-12:45 – Fellowship talks
- Hui Yu: Lipid binding properties and cellular functions of Nedd4-1 and Nedd4-2.
- Suman Ranjit: Conformational dynamics of nicked and gapped DNA using fluorescence backbone modifications.
- Kapil Bambardekar: Role of protein diffusion in gonadal patterning of C. elegans.
- Lunch
- 14:00-17:30 – Computer based training on biosensors and FLIM. Download computing tutorials and data files
- 17:30-18:00 – Special lecture 2. John Eid: Real-time DNA sequencing from single polymerase molecules.
- 18:15-20:00 – Banquet.
Thursday, October 29, 2009
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Enrico Gratton: The phasor approach to FLIM and FRET
- Break
- 11:00-11:45 – Lecture 13. Luca Lanzanò: Particle tracking
- 11:45-12:15 – Application 5. Chiara Stringari: Applications of FLIM.
- 12:15-12:45 – Discussion.
- Lunch
- 14:00-17:30 – Laboratory training on FCS. Download laboratory tutorials
Friday, October 30, 2009
- Breakfast
- 09:00-12:45 – Laboratory training on RICS and FLIM. Download laboratory tutorials
- 12:45 – Closure
Lecturers
- Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Michelle A Digman
Optical Biology Core Facility, University of California, Irvine, USA - John S Eid
Pacific Biosciences Inc., Menlo Park, USA - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Klaus Hahn
Department of Pharmacology, University of North Carolina at Chapel Hill, USA - Louis Hodgson
Department of Anatomy and Structural Biology, Albert Einstein College of Medicine of Yeshiva University, New York, USA - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Luca Lanzanò
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Pierre Moens
School of Science & Technology, University of New England, Armidale, Australia. - Giulia Ossato
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Susana A Sánchez
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Chiara Stringari
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Paul W Wiseman
Department of Physics and Chemistry, McGill University, Montreal, Canada
Registrants
- Seksiri Arttamangkul
Vollum Institute, Oregon Health & Science University, Portland, OR. - Kapil Bambardekar
Center for Complex Biological Systems, University of California Irvine, Irvine, CA. - Timothy Bilash
Practice of Dr. Timothy Bilash, Del Mar, CA. - William Birdsong
Vollum Institute, Oregon Health & Science University, Portland, OR. - Bruno Castro
Centro de Química-Física Molecular and Institute of Nanoscience, Instituto Superior Técnico, Lisboa, Portugal. - Joerg Engel
Software Development BioSciences, Carl Zeiss MicroImaging GmbH, Jena, Germany. - Bjoern Falkenburger
Physiology and Biophysics, University of Washington, Seattle, WA. - Felipe Montecinos Franjola
Biología, Laboratorio de Biología Estructural y Molecular, Santiago, Chile. - Emily Gibson
Physics, University of Colorado Denver, Denver, CO. - Hector Giral-Arnal
Renal Division, University of Colorado Denver, Aurora, CO. - Greg Glazner
Renal Division, University of Colorado Denver, Aurora, CO. - Sharona Gordon
Physiology and Biophysics, University of Washington, Seattle, WA. - Emmanuel Adu Gyamfi
Chemistry and Biochemistry, University of Notre Dame, South Bend, IN. - Tim Lei
Renal Division, University of Colorado Denver, Aurora, CO. - Moshe Levi
Renal Division, University of Colorado Denver, Aurora, CO. - Holger Lorenz
Zentrum für Molekulare Biologie, University Heidelberg, Heidelberg, Germany. - Marjolein Meddens
Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, Nijmegen, Netherlands. - Julia Philip
Chemistry and Biochemistry, University of Notre Dame, Notre Dame, IN. - Christopher Price
Center for Biomedical Engineering Research, Dept. of Mechanical Engineering, University of Delaware, Newark, DE. - Suman Ranjit
Single Molecule Biophysics, Biodesign Institute, Arizona State University, Tempe, AZ. - Samantha Schwartz
Nanoscience and Microsystems, University of New Mexico, Albuquerque, NM. - Jorge Bernardino de la Serna
MEMPHYS-Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark. - Carmen Ufret-Vincenty
Physiology and Biophysics, University of Washington, Seattle, WA. - Jianrong Wu
Pharmacology, University of North Carolina at Chapel Hill, Chapel Hill, NC. - Hui Yu
Chemistry and Biochemsitry, University of Notre Dame, Notre Dame, IN. - Li Zhang
Molecular and Cell Biology, University of Texas at Dallas, Richardson, TX.