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Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Group Photo
Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- GFP: Green Fluorescent Pasta
- ICS: Image Correlation Spectroscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- STICS: Spation-Temporal Image Correlation Spectroscopy
- more...
5th LFD Workshop (2010)
The "5th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 25–29, 2010, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 25, 2010
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Susana Sánchez: Introduction to scanning FCS (sFCS)
- 11:45-12:15 – Application 1. Enrico Gratton: Applications of FCS and ccFCS.
- 12:15-12:45 – Fellowship talks
- Xiaolin Tang: Vesicular stomatitis virus glycoprotein microdomain organization.
- Pei-I Ku: Live imaging of ESCRT recruitment in virus budding.
- Madeline Burgoyne: Application of RICS to examine the effect of RC3 on the diffusion of calmodulin.
- Lunch
- 14:00-17:30 – Computer based training on FCS. Download computing tutorials and data files
- 17:30-20:00 – Social event.
Tuesday, October 26, 2010
- Breakfast
- 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Michelle Digman: The number and brightness method (N&B)
- Break
- 11:00-11:45 – Lecture 6. Francesco Cardarelli: The pair correlation approach (pCF)
- 11:45-12:15 – Application 2. Elizabeth Hinde: Pair correlation on chromosomes.
- 12:15-12:45 – Application 3. Enrico Gratton: Measuring binding in vivo.
- Lunch
- 14:00-17:30 – Computer based training on RICS. Download computing tutorials and data files
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- 18:30-20:00 – Social event.
Wednesday, October 27, 2010
- Breakfast
- 09:00-09:45 – Lecture 7. Klaus Hahn: Biosensors and computational tools
- 09:45-10:30 – Lecture 8. Klaus Hahn: Recent developments: protein manipulation in vivo.
- Break
- 11:00-11:45 – Lecture 9. Enrico Gratton: Ratiometric FRET imaging
- 11:45-12:15 – Lecture 10. Michelle Digman: Cross-RICS and cross-N&B
- 12:15-12:45 – Fellowship talks
- Carlos Alberto Marques de Carvalho: Shedding light on the entry of a new world alphavirus into host cells.
- Julia Gunzenhäuser: Quantitative analysis of HIV-1 Gag clusters and the impact of fluorescently labelled Gag.
- Moussa Barhoum: Utilizing spot size analysis (SSA) in investigation of single molecule trajectories.
- Lunch
- 14:00-17:00 – Computer based training on biosensors and FLIM. Download computing tutorials and data files
- 17:00-17:45 – Special lecture 2. Kerry Hanson: Glowing skin: how two-photon fluorescence imaging is improving sun protection.
- 18:15-20:00 – Banquet dinner.
Thursday, October 28, 2010
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Enrico Gratton: The phasor approach to FLIM and FRET
- Break
- 11:00-11:45 – Application 4. David Jameson: Applications of phasors to in vitro studies of biomolecules.
- 11:45-12:15 – Application 5. Chiara Stringari: Phasor plot applied to tissues.
- 12:15-12:45 – Lecture 13. Luca Lanzanò: Particle tracking
- Lunch
- 14:00-17:30 – Laboratory training on FCS. Download laboratory tutorials
Friday, October 29, 2010
- Breakfast
- 09:00-12:15 – Laboratory training on RICS and FLIM. Download laboratory tutorials
- 12:15-12:45 – Closure
- Lunch
Lecturers
- Francesco Cardarelli
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Michelle A Digman
Optical Biology Core Facility, University of California, Irvine, USA - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Klaus Hahn
Department of Pharmacology, University of North Carolina at Chapel Hill, USA - Kerry Hanson
Department of Chemistry, University of California, Riverside, USA - Elizabeth Hinde
Optical Biology Core Facility, University of California, Irvine, USA - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Luca Lanzanò
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Susana A Sánchez
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Chiara Stringari
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
Registrants
- Moussa Barhoum
Chemistry, University of Utah, Salt Lake City, UT - Carol Bratt
Dental Research, University of Iowa, Iowa City, IA - Madeline Burgoyne
Biomedical Science, Neurobiology and Anatomy, University of Texas, Houston, TX - Carlos Alberto Marques de Carvalho
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Rio de Janeiro, Brazil - Eva Arnspang Christensen
MEMPHYS Center for Biomembrane Physics, University of Southern Denmark, Odense, Denmark - Rose Ellen Dixon
Physiology and Biophysics, University of Washington, Seattle, WA - Patton Garay
Biological Sciences, Allergan Inc., Irvine, CA - Harry Gill
Phyiology and Biophysics, Case Western Reserve University, Cleveland, OH - Julia Gunzenhäuser
Laboratoire de Biophysique Expérimenta, École Polytechnique Fédérale de Lausanne (EPFL), Lausanne, Switzerland - Christopher Hammang
School of Natural Sciences, University of Western Sydney, Sydney, Australia - Yanfei Jiang
Biochemistry and Molecular Biophysics, Washington University in St. Louis, St. Louis, MO - Ajit Joglekar
Biology, University of North Carolina, Chapel Hill, NC - Sander de Jong
Research and Development, Lambert Instruments, Roden, The Netherlands - Daichi Kamiyama
Biology, University of Miami, Miami, FL - Yujin Kim
Cellular Biochemistry, Max-Planck-Institut für Biochemie, Martinsried, Germany - Krzysztof Krawiec
Faculty of Physics, Division of Biophysics, Warsaw University, Warszawa, Poland - Pei-I Ku
Physics, University of Utah, Salt Lake City, UT - Moshe Levi
Renal Diseases and Hypertension, Health Sciences Center, University of Colorado at Denver, Aurora, CO - M Pilar Lillo
Quimica Física Biológica, Inst. Quimica Fisica, CSIC, Madrid, Spain - Xiangyi Luo
Physics/Biology, University of Utah, Salt Lake City, UT - Omid Masihzadeh
Bioengineering, University of Colorado at Denver, Aurora, CO - Joseph Mazurkiewicz
Center for Neuropharmacology and Neuroscience, Albany Medical College, Albany, NY - Radu Moldovan
Advanced Light Microscopy Core Facility, School of Medicine, University of Colorado at Denver, Aurora, CO - Rajesh Ramachandran
Physiology and Biophysics, Case Western Reserve University School of Medicine, Cleveland, OH - Justin Ross
Cell and Molecular Biology, University of Hawaii, Honolulu, HI - Fabrizio Salomone
Laboratorio NEST, Scuola Normale Superiore di Pisa, Pisa, Italy - Diana Schlamadinger
Chemistry and Biochemistry, University of California, San Diego, La Jolla, CA - Xiaolin Tang
Physics/Biology and Astronomy, University of Utah, Salt Lake City, UT - Dudley Williams
Biological Sciences, Allergan Inc., Irvine, CA - Ding Xu
Cellular and Molecular Medicine, University of California, San Diego, La Jolla, CA - Melanie Pribisko Yen
Chemistry, California Institute of Technology, Pasadena, CA - Tiefeng You
ISS Inc., Champaign, IL