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Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Group Photo
Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- more...
6th LFD Workshop (2011)
The "6th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 24–28, 2011, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 24, 2011
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Luca Lanzanò: Introduction to scanning FCS (sFCS)
- 11:45-12:30 – Application 1. Enrico Gratton: Applications of FCS.
- 12:30-12:45 – Posters
- Lunch
- 14:00-17:30 – Computer based training on FCS. Download computing tutorials and data files
- 17:30-20:00 – Social event.
Tuesday, October 25, 2011
- Breakfast
- 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Michelle Digman: The number and brightness method (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation method (pCF)
- 11:45-12:30 – Application 2. Elizabeth Hinde: Pair correlation in the nucleus.
- 12:30-12:45 – Posters
- Lunch
- 14:00-17:30 – Computer based training on RICS. Download computing tutorials and data files
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- 18:30-20:00 – Social event.
Wednesday, October 26, 2011
- Breakfast
- 09:00-09:45 – Lecture 7. Klaus Hahn: Biosensors and computational tools
- 09:45-10:30 – Lecture 8. Klaus Hahn: Photomodulation of proteins in living cells and animals
- Break
- 11:00-11:45 – Lecture 9. David Jameson: Introduction to fluorescence lifetimes and phasors
- 11:45-12:30 – Lecture 10. Enrico Gratton: Fluorescence Lifetime Imaging (FLIM)
- 12:30-12:45 – Posters
- Lunch
- 14:00-17:00 – Computer based training on biosensors and FLIM. Download computing tutorials and data files
- 17:30-18:00 – Special lecture 2. Enrico Gratton: Kicking the Gold.
- 18:00-20:00 – Banquet dinner.
Thursday, October 27, 2011
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Michelle Digman: Cross-RICS and cross-N&B
- Break
- 11:00-11:45 – Lecture 13. Luca Lanzanò: Particle tracking
- 11:45-12:30 – Application 3. Chiara Stringari: Phasor plot applied to tissues.
- 12:30-12:45 – Posters
- Lunch
- 14:00-17:30 – Laboratory training on FCS. Download laboratory tutorials
- 17:30-18:00 – Sponsor talk. Joseph Huff: Single molecule techniques using the Zeiss LSM 780.
- 18:00-20:00 – Social event.
Friday, October 28, 2011
- Breakfast
- 09:00-12:15 – Laboratory training on RICS and FLIM. Download laboratory tutorials
- 12:15-12:45 – Closure
- Lunch
Lecturers
- Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Klaus Hahn
Department of Pharmacology, University of North Carolina at Chapel Hill, USA - Elizabeth Hinde
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Joseph Huff
Laser Scanning Microscopy, Carl Zeiss MicroImaging - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Luca Lanzanò
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Chiara Stringari
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
Registrants
- Paolo Annibale
Laboratory of Nanoscale Biology, Interfaculty Institute of Bioengineering, Ecole Polytechnique Federale Lausanne, Lausanne, Switzerland. - Seksiri Arttamangkul
Vollum Institute, Oregon Health & Science University, Portland, OR. - Jerome Ballesta
Axiom Optics, Imagine Optic Inc, Cambridge, MA. - Corina Balut
School of Medicine, Cell Biology and Physiology, University of Pittsburgh, Pittsburgh, PA. - Antonella Battisti
National Enterprise for nanoScience and nanoTechnology (NEST), Scuola Normale Superiore, Pisa, Italy. - Ranieri Bizzarri
National Enterprise for nanoScience and nanoTechnology (NEST), Scuola Normale Superiore, Pisa, Italy. - Pina Colarusso
Physiology and Pharmacology, University of Calgary, Calgary, Canada. - Andres Mauricio Cardozo Gizzi
Centro de Investigaciones en Química Biológica de Córdoba, Universidad Nacional de Córdoba, Cordoba, Argentina. - Henry Haeberle
Dermatology, Columbia University, New York, NY. - Nicole Hynson
Ecology and Evolutionary Biology, University of California, Irvine, Irvine, CA. - Sofia Johansson
Applied Physics, Royal Institute of Technology, Stockholm, Sweden. - Malgorzata Kluba
Chemistry (Biochemistry, Molecular and Structural Biology Section), Katholieke Universiteit Leuven, Heverlee, Belgium. - Xiangduo Kong
Biological Chemistry, University of California, Irvine, Irvine, CA. - Mariagrazia Di Luca
National Enterprise for nanoScience and nanoTechnology (NEST), Scuola Normale Superiore, Pisa, Italy. - Alex Macmillan
Biomedical Imaging Facility, University of New South Wales, Sydney, Australia. - Stephen Mieruszynski
School of Natural Sciences, University of Western Sydney, Richmond, Australia. - Pradeep Nair
Molecular Oncology, Genentech, Inc., South San Francisco, CA. - Yauheni Novikau
BioSciences Division, Carl Zeiss MicroImaging GmbH, Jena, Germany. - Dylan Owen
Centre for Vascular Research, University of New South Wales, Sydney, Australia. - Hande Ozgen
Cell Membrane Biology, University Medical Center Groningen, Groningen, Netherlands. - Pabak Sarkar
Laboratory of Molecular Physiology, Section of Biophotonics, National Institute of Alcohol Abuse and Alcoholism, NIH, Bethesda, MD. - Jeffrey Sasaki
Institute of Virology and Immunology, The Gladstone Institutes, San Francisco, CA. - Barbara Alcaraz Silva
Beckman Laser Institute, University of California, Irvine, Irvine, CA. - Joshua Simpson
School of Natural Sciences, University of Western Sydney, Richmond, Australia. - Rosangela Sozzani
Biology, Duke University, Durham, NC. - Antonio Trullo
Centro Nacional de Investigaciones Cardiovasculares Carlos III, Madrid, Spain. - Andrea Ursic
National Enterprise for nanoScience and nanoTechnology (NEST), Scuola Normale Superiore, Pisa, Italy. - Klaus Weisshart
BioSciences Division, Carl Zeiss MicroImaging GmbH, Jena, Germany. - Cara Winter
Biology, Duke University, Durham, NC. - Shuang Wu
Biology, University of Pennsylvania, Philadelphia, PA. - Tiefeng You
ISS Inc., Champaign, IL. - Ivan Yudushkin
Cellular and Molecular Pharmacology, University of California, San Francisco, San Francisco, CA. - Hanxiang Zeng
Chemistry, University of California, Irvine, Irvine, CA.