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Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Group Photo
Abbreviations
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- more...
7th LFD Workshop (2012)
The "7th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 22–26, 2012, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 22, 2012
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Luca Lanzanò: Introduction to scanning FCS (sFCS)
- 11:45-12:15 – Application 1. Susana Sánchez: Applications of FCS.
- 12:15-12:35 – Application 2. Marcia Levitus: Applications of FCS.
- Lunch
- 14:00-17:30 – Computer based training on FCS. Download computing tutorials and data files
- 17:30-18:30 – Sponsor talk. Scott Jordan: Recent advancements in high-throughput interfacing of relevance to fluorescence microscopy.
- 18:30-20:00 – Social event.
Tuesday, October 23, 2012
- Breakfast
- 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Michelle Digman: The number and brightness method (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation method (pCF)
- 11:45-12:15 – Application 3. Francesco Cardarelli: Pair correlation in the nucleus.
- 12:15-12:35 – Posters
- Lunch
- 14:00-17:30 – Computer based training on RICS. Download computing tutorials and data files
- 17:30-18:30 – Special lecture. David Jameson: A nano-history of fluorescence
- 18:30-20:00 – Social event.
Wednesday, October 24, 2012
- Breakfast
- 09:00-09:45 – Lecture 7. Klaus Hahn: Biosensors and computational tools.
- 09:45-10:30 – Lecture 8. Klaus Hahn: Protein manipulation in vivo.
- Break
- 11:00-11:45 – Lecture 9. David Jameson: Introduction to fluorescence lifetimes and phasors
- 11:45-12:15 – Lecture 10. Enrico Gratton: Fluorescence Lifetime Imaging (FLIM)
- 12:15-12:35 – Application 4. Chiara Stringari: Phasor plot applied to tissues.
- Lunch
- 14:00-17:30 – Computer based training on biosensors and FLIM. Download computing tutorials and data files
- 18:30-20:00 – Banquet dinner.
Thursday, October 25, 2012
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Michelle Digman: Cross-RICS and cross-N&B
- Break
- 11:00-11:45 – Lecture 13. Luca Lanzanò: Particle tracking
- 11:45-12:15 – Discussion.
- 12:15-12:35 – Posters.
- Lunch
- 14:00-17:30 – Laboratory training on FCS. Download laboratory tutorials
- 17:30-20:00 – Social event.
Friday, October 26, 2012
- Breakfast
- 09:00-12:15 – Laboratory training on RICS, pCF and FLIM. Download laboratory tutorials
- 12:15-12:45 – Closure
- Lunch
Lecturers
- Francesco Cardarelli
Center for Nanotechnology Innovation, Istituto Italiano di Tecnologia, Pisa, Italy - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Klaus Hahn
Department of Pharmacology, University of North Carolina at Chapel Hill, USA - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Scott Jordan
Physik Instrumente, San Jose, California, USA - Luca Lanzanò
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Marcia Levitus
Department of Chemistry and Biochemistry, Arizona State University, USA - Susana Sánchez
Microscopy Unit, Centro Nacional de Investigaciones Cardiovasculares, Madrid, Spain - Chiara Stringari
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA
Registrants
- Ikram Blilou
Moleculaire Genetics, Utrecht University, Utrecht, The Netherlands. - Ron Broide
Biological Sciences, Allergan Inc., Irvine, CA. - Brian Cai
Biological Sciences, Allergan Inc., Irvine, CA. - Marcos Cantu
Interdepartmental Neuroscience Program, University of California, Irvine, Irvine, CA. - Jeffrey L Caplan
Delaware Biotechnology Institute, University of Delaware, Newark, DE. - Di Rienzo Carmine
National Enterprise for nanoScience and nanoTechnology (NEST), Scuola Normale Superiore, Pisa, Italy. - Stefano Coppola
Physics, Sapienza University of Rome, Rome, Italy. - Ulas C Coskun
Microscopy/Research, ISS Inc., Champaign, IL. - Patton Garay
Biological Sciences, Allergan Inc., Irvine, CA. - Tracy Handel
SSPPS/Pharmacology, University of California, San Diego, San Diego, CA. - Nicholas James
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Hongjiang Li
Plant Systems Biology, VIB - Ghent University, Gent, Belgium. - Sherry Liu
Biological Sciences, Allergan Inc., Irvine, CA. - Verena Mackowiak
R&D, Thorlabs GmbH, Dachau, Germany. - Pierre Moens
Science & Technology, University of New England, Armidale, Australia. - R Carlos Munoz-Garay
Instituto de Biotecnología, Cuernavaca, Mexico. - Aaron K Neumann
Pathology, University of New Mexico Health Sciences Center, Albuquerque, NM. - Christian Peter Poulsen
Plant Biology and Biotechnology, University of Copenhagen, Frederiksberg C, Denmark. - Diego Martin Presman
National Cancer Institute, National Institute of Health, Bethesda, MA. - Thomas E Rasmussen
Center for Biomembrane Physics (Memphys), University of Southern Denmark, Odense M, Denmark. - Catherine Rheaume
Biological Sciences, Allergan Inc., Irvine, CA. - Gordon Ruthel
PennVet Imaging Core, University of Pennsylvania School of Veterinary Medicine, Philadelphia, PA. - Bryan Stephens
Pharmacology, University of California, San Diego, San Diego, CA. - Haibing Teng
Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, PA. - Damaris De La Torre
Center of Drug Discovery and Biodiversity, INDICASAT AIP Panama Research Institute of Science and Medicine, Panama, Panama. - Carissa Vetromile
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Matthew Weitzman
Biological Sciences, University of Delaware, Newark, DE. - Robert T Youker
Renal-Electrolyte Division, University of Pittsburgh, Pittsburgh, PA.