Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- more...
8th LFD Workshop (2013)
The "8th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 21–25, 2013, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 21, 2013
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Michelle Digman: Introduction to scanning FCS (sFCS)
- 11:45-12:15 – Application 1. Susana Sánchez: Applications of FCS.
- 12:15-12:35 – Poster presentations.
- Lunch
- 14:00-17:30 – Computer based training on FCS. Download computing tutorials and data files
- 17:30-18:30 – Sponsor talk. Olivier Brun: The Leica TCS SP8 microscope.
- 18:30-20:00 – Social event.
Tuesday, October 22, 2013
- Breakfast
- 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Michelle Digman: The number and brightness method (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation method (pCF)
- 11:45-12:15 – Application 2. Elizabeth Gordon: Application of image correlation analysis
- 12:15-12:35 – Application 3. Niklas Hedde: Super-resolution STED microscopy
- Lunch
- 14:00-17:30 – Computer based training on RICS and FLIM. Download computing tutorials and data files
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- 18:30-20:00 – Social event.
Wednesday, October 23, 2013
- Breakfast
- 09:00-09:45 – Lecture 7. David Jameson: Introduction to fluorescence lifetimes and phasors
- 09:45-10:30 – Lecture 8. Enrico Gratton: Fluorescence Lifetime Imaging (FLIM)
- Break
- 11:00-11:45 – Lecture 9. Alexander Dvornikov and Enrico Gratton: 3D imaging and FLIM
- 11:45-12:15 – Lecture 10. Enrico Gratton: Spectral phasors
- 12:15-12:35 – Application 4. Sohail Jahid: FLIM in animal models.
- Lunch
- 13:30-14:00 – Sponsor talk. Mark Eastman: The Olympus IX3 inverted research microscope line.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials
- 18:30-20:00 – Banquet dinner.
Thursday, October 24, 2013
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Michelle Digman: Cross-RICS and cross-N&B
- Break
- 11:00-11:45 – Lecture 13. Paolo Annibale: Particle tracking
- 11:45-12:15 – Application 5. Paolo Annibale and Andrea Anzalone: Application of particle tracking.
- 12:15-12:35 – Discussion.
- Lunch
- 14:00-17:30 – Laboratory training. Download laboratory tutorials
- 17:30-18:30 – Special lecture 2. Marcia Levitus: FCS study of the oligomerization dynamics of two DNA replication processivity clamps
- 18:30-20:00 – Social event.
Friday, October 25, 2013
- Breakfast
- 09:00-12:15 – Laboratory training. Download laboratory tutorials
- Lunch
- 12:35-14:00 – Symposium
- 14:00-15:30 – Group presentations
Lecturers
- Paolo Annibale
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Andrea Anzalone
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Olivier Brun
Capital Equipment Sales, Leica Microsystems, USA - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Mark Eastman
Microscope Sales, Olympus America, Inc., USA - Elizabeth Gordon
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - Sohail Jahid
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa, USA - Marcia Levitus
Department of Chemistry and Biochemistry. Arizona State University, USA - Susana Sánchez
Facultad de Ciencias Quimicas, Departamento de Polimeros, Universidad de Concepcion, Chile
Registrants
- Shailesh R Agarwal
Pharmacology, School of Medicine, University of Nevada, Reno, NV. - Shane Andrews
Scientific Equipment Group, Olympus America, Center Valley, CA. - Umme Ayesa
Biochemistry, Temple University, Philadelphia, PA. - Michael Dan Brenner
National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD. - Ulas Coskun
Microscopy, ISS Inc., Champaign, IL. - Kyle Ellefsen
Neurobiology & Behavior, University of California, Irvine, CA. - Patton Garay
Biological Sciences, Allergan, Irvine, CA. - Adán Guerrero
Laboratorio Nacional de Microscopía Avanzada, Universidad Nacional Autónoma de México, Cuernavaca, Mexico. - Geoffrey Harlow
Botany and Plant Sciences, University of California, Riverside, CA. - Laurel Hoffman
Neurobiology and Anatomy, University of Texas Health Science Center, Houston, TX. - Jerome Hottin
Biophysics, Centre de Biochimie Structurale (CBS), Montpellier, France. - Feng Li
Pharmaceutical Sciences, University of California, Irvine, CA. - Nadir Planes
Pharmacology, Instituto de Investigaciones Científicas y Servicios de Alta Tecnología, Panama. - Kevin W Rodriguez
Botany and Plant Sciences, University of California, Riverside, CA. - Galina Schmunk
Physiology and Biophysics, University of California, Irvine, CA. - Smita Soni
School of Medicine, Indiana University, South Bend, IN. - Elina Staaf
Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden. - Kerry Swift
Molecular Binding Characterization, Abbott Laboratories, Abbott Park, IL. - Henrik Seir Thoke
Center for Biomembrane Physics (MEMPHYS), University of Southern Denmark, Odense, Denmark. - Viviana I Torres
Centro de Microscopia Avanzada, University of Concepcion, Chile. - Jiangcheng Wang
Marketing Group, Carl Zeiss Microscopy, LLC, Thornwood, NY. - Christopher Wood
Laboratorio Nacional de Microscopía Avanzada, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Mexico. - Haisheng Yang
Basic Medical Sciences, Purdue University, West Lafayette, IN. - Ai Zhang
Integrative Structural and Molecular Biology, The Scripps Research Institute, La Jolla, CA.