Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Calendars
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- more...
9th LFD Workshop (2014)
The "9th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 27–31, 2014, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 27, 2014
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Introduction to fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Michelle Digman: Introduction to scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Fluorescence polarization.
- 12:15-12:35 – Posters.
- Lunch
- 13:30-14:00 – Application talk 2. Per Niklas Hedde: Single plane illumination microscopy (SPIM)
- 14:00-17:30 – Computer training on FCS. Download computing tutorials and data files .
- 17:30-18:00 – Sponsor talk 1. Carlos Alonso (Leica): Super resolution imaging with GSD (ground state depletion).
- 18:00-18:30 – Sponsor talk 2. Nick J Dolman (Life Technologies): Engineering fluorescence.
- Social event
Tuesday, October 28, 2014
- Breakfast
- 09:00-09:45 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Per Niklas Hedde: The number and brightness method (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation approach (pCF)
- 11:45-12:15 – Application talk 3. Carmine Di Rienzo: The iMSD approach to diffusion analysis.
- 12:15-12:35 – Application talk 4. Suman Ranjit: Applications of FCS.
- Lunch
- 13:30-14:00 – Application talk 5. Michelle Digman: Applications of N&B.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence.
- Social event
Wednesday, October 29, 2014
- Breakfast
- 09:00-09:45 – Lecture 7. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 8. Enrico Gratton: 3-Dimensional phasors in DIVER
- Break
- 11:00-11:45 – Lecture 9. Enrico Gratton: Spectral phasors
- 11:45-12:15 – Application talk 6. Marian Waterman: Applications of FLIM.
- 12:15-12:35 – Application talk 7. Rupsa Datta: FLIM in cardiomyocytes.
- Lunch
- 13:30-14:00 – Application talk 8. Andrea Anzalone: Applications of FLIM.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 17:30-18:00 – Sponsor talk 3. Jeremy Pronchik (Horiba): Fluorescence applications.
- 18:00-18:30 – Sponsor talk 4. Eunah Lee (Horiba): Confocal Raman microscopy.
- Social event
Thursday, October 30, 2014
- Breakfast
- 09:00-09:45 – Lecture 10. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 11. Enrico Gratton: Cross-RICS and cross-N&B
- Break
- 11:00-11:45 – Lecture 12. Paolo Annibale: Particle tracking
- 11:45-12:15 – Application talk 9. Chi-Li Chiu: Applications of particle tracking.
- 12:15-12:35 – Application talk 10. Alexander Dvornikov: Deep tissue imaging (DIVER).
- Lunch
- 13:30-14:00 – Application talk 11. Rachel Cinco: FLIM in tissues.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 17:30-18:00 – Application talk 12. Alessandro Rossetta: The FLIMbox principle.
- 18:00-18:30 – Application talk 13. Paolo Annibale: Orbital tracking in the nucleus.
- 18:30-20:00 – Banquet dinner.
Friday, October 31, 2014
- Breakfast
- 09:00-12:15 – Laboratory training. Download laboratory tutorials .
- 12:15-12:35 – Symposium.
- Lunch
- 13:30-14:00 – Symposium.
- 14:00-15:30 – Group presentations.
- 15:30-16:00 – Conclusion.
Lecturers
- Carlos Alonso
Leica Microsystems (Sponsor) - Paolo Annibale
Laboratory for Fluorescence Dynamics, University of California, Irvine - Andrea Anzalone
Laboratory for Fluorescence Dynamics, University of California, Irvine - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine - Chi-Li Chiu
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Rachel Cinco
Developmental & Cell Biology, University of California, Irvine - Rupsa Datta
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Nick J Dolman
Life Technologies (Sponsor) - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Eunah Lee
Horiba Scientific (Sponsor) - Jeremy Pronchik
Horiba Scientific (Sponsor) - Carmine Di Rienzo
National Enterprise for Nano-Science and Nano-Technology, Italy - Alessandro Rossetta
Laboratory for Fluorescence Dynamics, University of California, Irvine - Marian Waterman
Microbiology and Molecular Genetics, University of California, Irvine
Registrants
- Swathi Bagilthaya
Biomedical Engineering, University of California, Irvine - Lee Bardwell
Developmental & Cell Biology, University of California, Irvine - Arunima Bhattacharjee
Chemical Engineering and Materials Science, University of California, Irvine - Arianna Ceccarelli
Experimental Medicine and Surgery, University of Rome Tor Vergata, Rome, Italy - Anna Celli
University of California, San Francisco - Angela Garcia
Hematology/Oncology, University of California, Irvine - Michael Ignatov
BDAS, Allergan LLC, Irvine, CA - Joseph Larkin
Physics, Northeastern University, Dorchester, MA - Feng Liu-Smith
Epidemiology, University of California, Irvine - Emma Mah
Chemical Engineering, University of California, Irvine - Viktor Malyarchuk
Microscopy, ISS Inc, Champaign, IL - Guillermo Marques
University Imaging Centers, University of Minnesota, Minneapolis, MN - Maria Bloksgaard Moelgaard
Cardiovascular and Renal Research, University of Southern Denmark, Institute of Molecular Medicine, Odense C, Denmark - Michael Murata
Biomedical Engineering, University of California, Irvine - Steffen B Petersen
Health Science and Technology, Ualborg University, Aalborg, Denmark - Ramon Roca Pinilla
Biomedical Engineering, University of California, Irvine - Jennifer Sasaki Russell
Neuroscience, Tufts University, Boston, MA - Krzysztof Sozanski
Department of Soft Condensed Matter, Institute of Physical Chemistry, Polish Academy of Sciences, Warsaw, Poland - Yuansheng Sun
Microscopy, ISS Inc, Champaign, IL - Katherine Barbara Szarama
Cell and Molecular Biology, St. Jude Children's Research Hospital, Memphis, TN - Andrew Trinh
Biomedical Engineering, University of California, Irvine - Betty Wong
Chemistry, California Institute of Technology, Pasadena, CA - Henry Wu
Medicine, Mount Sinai Hospital, New York, NY