Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Calendars
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- more...
10th LFD Workshop (2015)
The "10th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 26–30, 2015, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 26, 2015
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Michelle Digman: Scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Fluorescence polarization
- 12:15-12:35 – Posters.
- Lunch
- 13:30-14:00 – Application talk 2. Per Niklas Hedde: Single plane illumination microscopy (SPIM)
- 14:00-14:20 – Sponsor talk 1. Shane Andrews (Olympus): New developments at Olympus.
- 14:20-17:30 – Computer training on FCS. Download computing tutorials and data files .
- 17:30-18:00 – Sponsor talk 2. Chris Shumate (Etaluma): Microscopy simplified.
- Social event
Tuesday, October 27, 2015
- Breakfast
- 09:00-09:45 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Per Niklas Hedde: Number and brightness analysis (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation approach (pCF)
- 11:45-12:15 – Application talk 3. Enrico Gratton: The iMSD approach to diffusion analysis.
- 12:15-12:35 – Application talk 4. Suman Ranjit: Applications of FCS.
- Lunch
- 13:30-14:00 – Application talk 5. Michelle Digman: Applications of N&B.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- Social event
Wednesday, October 28, 2015
- Breakfast
- 09:00-09:45 – Lecture 7. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 8. Enrico Gratton: 3-Dimensional phasors in DIVER
- Break
- 11:00-11:45 – Lecture 9. Enrico Gratton: Spectral phasors
- 11:45-12:15 – Application talk 6. Agua Sobrino: Applications of FLIM.
- 12:15-12:35 – Application talk 7. Rupsa Datta: ROS detected by FLIM.
- Lunch
- 13:30-14:00 – Application talk 8. Hongtao Chen: FLIM with camera.
- 14:00-17:00 – Laboratory training. Download laboratory tutorials .
- 17:00-17:15 – Application talk 9. Leonel Malacrida: Laurdan spectral phasors.
- 18:30-20:00 – Banquet at Las Brisas, Laguna Beach.
Thursday, October 29, 2015
- Breakfast
- 09:00-09:45 – Lecture 10. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 11. Enrico Gratton: Cross-RICS and cross-N&B
- Break
- 11:00-11:45 – Lecture 12. Paolo Annibale: Particle tracking
- 11:45-12:15 – Application talk 10. Alexander Dvornikov: Deep tissue imaging (DIVER).
- 12:15-12:35 – Application talk 11. Suman Ranjit: Applications of DIVER.
- Lunch
- 13:30-14:00 – Application talk 12. Rachel Cinco: FLIM in tissues.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 17:30-18:00 – Application talk 13. Alessandro Rossetta: The FLIMbox principle.
- 18:00-18:30 – Application talk 14. Paolo Annibale: Tracking in the nucleus.
- Social event
Friday, October 30, 2015
- Breakfast
- 09:00-12:15 – Laboratory training. Download laboratory tutorials .
- 12:15-12:35 – Symposium.
- Lunch
- 13:30-14:00 – Symposium.
- 14:00-15:30 – Group presentations.
- 15:30-16:00 – Conclusion.
Lecturers
- Shane Andrews
Olympus America (Sposnsor) - Paolo Annibale
Laboratory for Fluorescence Dynamics, University of California, Irvine - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine - Rachel Cinco
Developmental & Cell Biology, University of California, Irvine - Rupsa Datta
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Leonel Malacrida
Laboratory for Fluorescence Dynamics, University of California, Irvine - Suman Ranjit
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alessandro Rossetta
Laboratory for Fluorescence Dynamics, University of California, Irvine - Chris Shumate
Etaluma, Inc. (Sponsor) - Agua Sobrino
Molecular Biology & Biochemistry, University of California, Irvine
Registrants
- Saif Al-Juboori
Electrical Engineering, University of Colorado Denver, CO. - Jorge Isaac Ceballos Alfonso
Microscopy, Smithsonian Tropical Research Institute, Panamá. - Filippo Begarani
Biophyiscs, Scuola Normale Superiore di Pisa, Italy. - Colton Gerard Boudreau
Physiology, McGill University, Canada. - Brian Cai
Biological Sciences, Allergan, Inc., CA. - Ulas Coskun
ISS Inc., IL. - Rhonda Davey
School of Science & Technology, University of New Englande, Armidale, Australia. - Evgenia Dobrinskikh
School of Medicine, Renal Diseases and Hypertension, University of Colorado Denver, CO. - Stefania Forner
Neurobiology and Behavior, University of California, Irvine, CA. - Walter Gabriel Gonzalez
Chemistry and Biochemistry, Florida International University, FL. - Andrew Emon Heidari
Beckman Laser Institute, University of California, Irvine, CA. - Megumi Katoh
Ph.D. Program in Human Biology, University of Tsukuba, Japan. - Wonbae Lee
Physics and Astronomy, University of California, Irvine, CA. - Sakura Eri Maezono
Graduate School of Integrative and Global Majors, University of Tsukuba, Japan. - Sara Makaremi
School of Biomedical Engineering, McMaster University, Canada. - Alessandra Cadete Martini
Neurobiology and Behavior, University of California, Irvine, CA. - Denys Marushchak
Physics and Astronomy, University of California, Irvine, CA. - Ingrid Linnea Olofsson
Institute for Research in Cancer and Immunology, Montreal University, Canada. - Jorge Bernardino de la Serna
Central Laser Facility, Research Complex at Harwell, United Kingdom. - Kazuyo Takeda
Microscopy and Imaging Core Facility, Food and Drug Administration, MD. - Steven J. Tobin
Molecular Medicine, Beckman Research Institute - City of Hope, CA. - Eszter Toth
Ph.D. Program in Human Biology, University of Tsukuba, Japan. - Tse-Luen Wee
Advanced BioImaging Facility, McGill University, Canada. - Lijuan Zhang
Campus Science Support Facilities GmbH, Austria.