Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Big Data Workshop
The Big Data Image Processing and Analysis workshop will be held on October 31 – November 4, 2016.
Calendars
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- more...
11th LFD Workshop (2016)
The "11th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 24–28, 2016, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 24, 2016
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Margaux Bouzin: Scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Fluorescence polarization
- 12:15-12:35 – Posters.
- Lunch
- 13:30-14:00 – Application talk 2. Per Niklas Hedde: Single plane illumination microscopy (SPIM)
- 14:00-14:20 – Sponsor talk 1. Mark Eastman (Olympus): Title TBA.
- 14:20-17:30 – Computer training on FCS. Download computing tutorials and data files .
- 17:30-18:00 – Sponsor talk 2. Nimesh Juthani (PCO): Title TBA.
- 18:00-18:30 – Sponsor talk 3. Scott Metzler (Photometrics): Title TBA.
- Social event
Tuesday, October 25, 2016
- Breakfast
- 09:00-09:45 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Per Niklas Hedde: Number and brightness analysis (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation approach (pCF)
- 11:45-12:15 – Application talk 3. Enrico Gratton: The iMSD approach to diffusion analysis.
- 12:15-12:35 – Application talk 4. Suman Ranjit: Applications of FCS.
- Lunch
- 13:30-14:00 – Application talk 5. Enrico Gratton: Applications of N&B.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- Social event
Wednesday, October 26, 2016
- Breakfast
- 09:00-09:45 – Lecture 7. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 8. Enrico Gratton: 3-Dimensional phasors in DIVER
- Break
- 11:00-11:45 – Lecture 9. Leonel Malacrida: Spectral phasors
- 11:45-12:15 – Application talk 6. Agua Sobrino: Applications of FLIM.
- 12:15-12:35 – Application talk 7. Rupsa Datta: ROS detected by FLIM.
- Lunch
- 13:30-14:00 – Application talk 8. Hongtao Chen: FLIM with cameras.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 18:30-20:00 – Banquet at Las Brisas, Laguna Beach.
Thursday, October 27, 2016
- Breakfast
- 09:00-09:45 – Lecture 10. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 11. Enrico Gratton: Particle tracking
- Break
- 11:00-11:45 – Application talk 9. Alexander Dvornikov: Deep tissue imaging (DIVER).
- 11:45-12:15 – Application talk 10. Suman Ranjit: Applications of DIVER.
- 12:15-12:35 – Lecture 12. Michelle Digman: Cross-RICS and cross-N&B
- Lunch
- 13:30-14:00 – Application talk 11. Ning Ma and Rachel Cinco: FLIM in tissues.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 17:30-18:00 – Application talk 12. Enrico Gratton: Tracking in the nucleus.
- Social event
Friday, October 28, 2016
- Breakfast
- 09:00-12:15 – Laboratory training. Download laboratory tutorials .
- 12:15-12:35 – Symposium.
- Lunch
- 13:30-15:30 – Group presentations.
- 15:30-16:00 – Conclusion.
Lecturers
- Margaux Bouzin
Laboratory for Fluorescence Dynamics, University of California, Irvine - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine - Rachel Cinco
Developmental & Cell Biology, University of California, Irvine - Rupsa Datta
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Mark Eastman
Olympus America Inc (Sponsor) - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Nimesh Juthani
PCO-TECH Inc (Sponsor) - Ning Ma
Laboratory for Fluorescence Dynamics, University of California, Irvine - Leonel Malacrida
Laboratory for Fluorescence Dynamics, University of California, Irvine - Scott Metzler
Photometrics Inc (Sponsor) - Suman Ranjit
Laboratory for Fluorescence Dynamics, University of California, Irvine - Agua Sobrino
Molecular Biology & Biochemistry, University of California, Irvine
Registrants
- Alessio Andreoni
LMB - Optical Spectroscopy Section, NHLBI/NIH, ND. - Priyam Banerjee
Thoracic/ head & Neck Medical Oncology, MD Anderson Cancer Center, TX. - Paolo Beuzer
Protein Biology, Vertex Pharmaceuticals, CA. - Richard Brizendine
Department of Pharmacology, University of Nevada Reno School of Medicine, NV. - Yun Seok Choi
Biochemistry and Molecular Biology, Colorado State University, CO. - Natalie Clark
Department of Plant and Microbial Biology, North Carolina State University, NC. - Clarence Dunn
Clinical Biomarker Discovery & Development, Amgen Inc., CA. - Angel German Velasco Felix
Laboratorio Nacional de Microscopia Avanzada, Instituto de Biotecnologia, UNAM, Mexico. - Claudia Leticia Gomez Flores
Laboratorio de Biofisica de Proteínas, Instituto de Fisica/ Universidad Autonoma de San Luis Potosi, Mexico. - Roxanne Glazier
Department of Biomedical Engineering, Georgia Institute of Technology and Emory University , GA. - Taras Golota
tRED R&D Technology and Applied Research, Ventana Medical Systems, AZ. - Daniel Holland
Fraser Lab / Translational Imaging Center, USC, CA. - Brett A. Johnson
Pathology & Laboratory Medicine, University of California, Irvine, CA. - Jason Junge
University of Southern California, CA. - Kevin Keomanee-Dizon
Translational Imaging Center, University of Southern California, CA. - Eun Sang "Daniel" Koo
Translational Imaging Center, University of Southern California, CA. - Erik Malarkey
Core, Vertex Pharmaceuticals, CA. - Courtney McCann
Physiology, Johns Hopkins School of Medicine, MD. - Molly Molloy
NHLBI, National Institutes of Health, MD. - Felipe Montecinos-Franjola
NICHD, National Institutes of Health, MD. - Rodrigo Alain Migueles Ramirez
Laboratorio Nacional de Microscopia Avanzada (LNMA), Instituto de Biotecnologia - UNAM, Mexico. - Francesco Reina
Weatherall Institute of Molecular Medicine, University of Oxford, United Kingdom. - Brendon Riekert
Biochemistry, University of Stellenbosch, South Africa. - Suparna Sarkar
Integrated Biology and Pharmacology, University of Texas Health Science Center at Houston, TX. - Francesco Scavone
Biochemistry & Molecular Biology, Colorado State University, CO. - Bjarne Thorsted
Department of Biochemistry and Molecular Biology, University of Southern Denmark, Denmark. - Josh Titlow
Biochemistry, Oxford University, United Kingdom.