Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Calendars
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- more...
12th LFD Workshop (2017)
The "12th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 23–27, 2017, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 23, 2017
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Margaux Bouzin: Scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Introduction to Phasors
- 12:15-12:35 – Posters.
- Lunch
- 13:30-14:00 – Application talk 2. Suman Ranjit: Applications of FCS.
- 14:00-17:30 – Computer training on FCS. Download computing tutorials and data files .
- Dinner
Tuesday, October 24, 2017
- Breakfast
- 09:00-09:45 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Per Niklas Hedde: Number and brightness analysis (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation approach (pCF)
- 11:45-12:15 – Lecture 7. Michelle Digman: Cross-RICS and cross-N&B
- 12:15-12:35 – Application talk 3. Per Niklas Hedde: Single plane illumination microscopy (SPIM).
- Lunch
- 13:30-14:00 – Application talk 4. Michelle Digman: Applications of N&B.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- Dinner
Wednesday, October 25, 2017
- Breakfast
- 09:00-09:45 – Lecture 8. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 9. Enrico Gratton: 3-Dimensional phasors in DIVER
- Break
- 11:00-11:45 – Lecture 10. Leonel Malacrida: Spectral phasors
- 11:45-12:15 – Application talk 5. Rachel Cinco-Hedde: Applications of FLIM.
- 12:15-12:35 – Application talk 6. Ning Ma: Embryo development by FLIM.
- Lunch
- 13:30-14:00 – Application talk 7. Hongtao Chen: FLIM with camera.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 18:30-20:00 – Banquet at Las Brisas, Laguna Beach.
Thursday, October 26, 2017
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Enrico Gratton: Particle tracking
- Break
- 11:00-11:45 – Application talk 8. Alexander Dvornikov: Deep tissue imaging (DIVER).
- 11:45-12:15 – Application talk 9. Suman Ranjit: Applications of DIVER.
- 12:15-12:35 – Application talk 10. Enrico Gratton: The iMSD approach to diffusion analysis
- Lunch
- 13:30-14:00 – Application talk 11. Michael Murata: FLIM in cells.
- 14:00-17:30 – Laboratory training. Download laboratory tutorials .
- 17:30-18:00 – Application talk 12. Enrico Gratton: Applications of the Airy detector.
- Dinner
Friday, October 27, 2017
- Breakfast
- 09:00-12:15 – Laboratory training. Download laboratory tutorials .
- 12:15-12:35 – Symposium.
- Lunch
- 13:30-15:30 – Group presentations.
- 15:30-16:00 – Conclusion.
Lecturers
- Margaux Bouzin
Laboratory for Fluorescence Dynamics, University of California, Irvine - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine - Rachel Cinco-Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Ning Ma
Laboratory for Fluorescence Dynamics, University of California, Irvine - Leonel Malacrida
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michael Murata
Laboratory for Fluorescence Dynamics, University of California, Irvine - Suman Ranjit
Laboratory for Fluorescence Dynamics, University of California, Irvine
Registrants
- Eduardo Anaya
Pathology, University of New Mexico, NM. - Hadel Al Asafen
Chemical and Biomolecular Engineering, NC State University, NC. - Carlos Cabrera
Facultad de Ingenieria, Universidad Autónoma de Baja California, Mexico. - Samir Pereira da Costa Campos
Instituto de Bioquímica Médica, Universidade Federal do Rio de Janeiro, Brazil. - Matthew Ferguson
Physics, Boise State University, ID. - Tara Gallagher
Molecular Biology & Biochemistry, University of California, Irvine, CA. - Vimal Gangadharan
Zeiss Customer Center NY, Carl Zeiss Microscopy, NY. - Mehran Ghafari
Computer Science, University of Tennessee at Chattanooga, TN. - Neeraj Gohad
Carl Zeiss Microscopy, NY. - Melissa Goodlad
Chemistry & Chemical Biology, University of California, Merced, CA. - David Silverio Moreno Gutiérrez
Departamento de Medicina Molecular y Bioprocesos, Instituto de Biotecnología - UNAM, Mexico. - Liu Jun
Mechanobiology Institute, National University of Singapore, Singapore. - Rosa Kaviani
Bioengineering, McGill University, Canada. - Sumire Matsumoto
Human Biology, School of Integrative and Global Majors, University of Tsukuba, Japan. - Zbigniew Mikulski
Microscopy Core, La Jolla Institute for Allergy and Immunology, CA. - Timothy Murphy-Stevens
Microscopy & Image Processing Pipeline, Allen Institute for Cell Science, WA. - Peng Qiwen
Mechanobiology Institute, National University of Singapore, Singapore. - Vanessa Pimenta Rocha
Departamento de Bioquímica, Universidade Federal do Estado do Rio de Janeiro, Brazil. - Joann Rodriguez
Civil and Environmental Engineering, University of Massachusetts Amherst, MA. - Florencia Sardi
Redox Biology of Trypanosomes, Institut Pasteur de Montevideo, Uruguay. - Shiqun Shao
Chemistry, University of California, Riverside, CA. - Wen Shi
Translational Imaging Center, University of Southern California, CA. - Rebika Shrestha
Ras Initiative, Leidos Biomedical Research, Inc, MD. - Diana Stavreva
Laboratory of Receptor Biology and Gene Expression, National Cancer Institute (NCI), National Institutes of Health (NIH), MD. - Dominik Stich
Pharmacology, University of Colorado Anschutz Medical Campus, CO. - Min Xue
Chemistry, University of California, Riverside, CA.