Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Calendars
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- STED: Stimulated Emission Depletion
- more...
13th LFD Workshop (2018)
The "13th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 22–26, 2018, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 22, 2018
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Lorenzo Scipioni: Scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Introduction to Phasors.
- 12:15-12:35 – Posters.
- Lunch
- 13:30-14:00 – Application talk 2. Suman Ranjit: Applications of FCS.
- 14:00-17:30 – Computer training on FCS. Download computing tutorials and data files .
- 17:30-17:50 – Sponsor talk 1. Giulia Ossato (Leica): SP8 FALCON, beyond spectral imaging.
- 17:50-18:10 – Sponsor talk 2. Scott Metzler (PCO Tech): sCMOS, the most versatile imaging tool in science.
- Dinner
Tuesday, October 23, 2018
- Breakfast
- 09:00-09:45 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Lorenzo Scipioni: Number and brightness analysis (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation approach (pCF)
- 11:45-12:15 – Lecture 7. Michelle Digman: Cross-RICS and cross-N&B
- 12:15-12:35 – Application talk 3. Per Niklas Hedde: Single plane illumination microscopy (SPIM).
- Lunch
- 13:30-14:00 – Application talk 4. Michelle Digman: Applications of N&B.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- Dinner
Wednesday, October 24, 2018
- Breakfast
- 09:00-09:45 – Lecture 8. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 9. Michelle Digman: FLIM
- Break
- 11:00-11:45 – Lecture 10. Leonel Malacrida: Spectral phasors
- 11:45-12:15 – Application talk 5. Rachel Cinco-Hedde: Applications of FLIM.
- 12:15-12:35 – Application talk 6. Ning Ma: Embryo development by FLIM.
- Lunch
- 13:30-14:00 – Application talk 7. Hongtao Chen: FLIM with cameras.
- 14:00-17:30 – Laboratory training.
- 18:30-20:00 – Banquet at Avila's El Ranchito, Newport Beach.
Thursday, October 25, 2018
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Alexander Vallmitjana Lees: Particle tracking
- Break
- 11:00-11:45 – Application talk 8. Alexander Dvornikov: Deep tissue imaging (DIVER).
- 11:45-12:15 – Application talk 9. Suman Ranjit: Applications of DIVER.
- 12:15-12:35 – Application talk 10. Enrico Gratton: The iMSD approach to diffusion analysis.
- Lunch
- 13:30-14:00 – Application talk 11. Enrico Gratton: Orbital tracking in cells.
- 14:00-17:30 – Laboratory training.
- 17:30-17:45 – Sponsor talk 3. Beniamino Barbieri (ISS Inc): A short presentation of our products and their application.
- 17:45-18:30 – Application talk 12. Lorenzo Scipioni: Applications of the Airy detector.
- Dinner
Friday, October 26, 2018
- Breakfast
- 09:00-12:15 – Laboratory training.
- 12:15-12:35 – Symposium.
- Lunch
- 13:30-15:30 – Group presentations.
- 15:30-16:00 – Conclusion.
Lecturers
- Beniamino Barbieri
ISS (Instrumenzione Scientificia Sperimentale) Inc., Champaign, IL - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine - Rachel Cinco-Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Alexander Vallmitjana Lees
Laboratory for Fluorescence Dynamics, University of California, Irvine - Ning Ma
Laboratory for Fluorescence Dynamics, University of California, Irvine - Leonel Malacrida
Laboratory for Fluorescence Dynamics, University of California, Irvine - Scott Metzler
PCO-Tech, WA - Giulia Ossato
Leica Microsystems Inc., Germany - Suman Ranjit
Laboratory for Fluorescence Dynamics, University of California, Irvine - Lorenzo Scipioni
Laboratory for Fluorescence Dynamics, University of California, Irvine
Registrants
- Rayna Addabbo
Chemistry, University of Wisconsin-Madison, WI. - Basello Davide Alessandro
Laboratory of RNA Biology, Institute of Molecular Genetics, Czech Republic. - Tala Allbutt
ACMM, University of Sydney, Australia. - Josue A Benjamin-Rivera
Chemistry, University of Puerto Rico - Rio Piedras, Puerto Rico. - Timothy Bilash
La Jolla, CA. - Chris Bjornsson
Carl Zeiss Microscopy LLC, NY. - Shanshan Cai
Biomedical Engineering, University of Southern California, CA. - Hsiao Ju Chaing
Translational Imaging Center, University of Southern California, CA. - Elizabeth Hinde
University of Melbourne, Australia. - Vidanage Nuwan Charitha Karunaratne
Costa Mesa, CA. - Sharon King
Light Microscopy Center, St Jude Children's Research Hospital, TN. - Nikita Kumari
School of Molecular Sciences, Arizona State University, AZ. - Adán Lara
Cellular and Molecular Neurobiology, Universidad Nacional Autónoma de México, México. - Kaitao Li
Biomedical Engineering, Georgia Institute of Technology, GA. - Maximilian Matthies
Cardiovascular and Renal Research, University of Southern Denmark, Institute for Molecular Medicine, Denmark. - Mehrnaz Mehrabi
Biomedical Engineering, University of California, Irvine, CA. - Fatemeh Jelvhei Moghaddam
Pharma, MultiOmyx, NeoGenomics Laboratories, CA. - Abira Rajah
Physiology, McGill University, Canada. - Saba Ranjbar
Chemical Engineering and Materials Science, University of California, Irvine, CA. - Yevgeniy Romin
Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, NY. - Jorge Bernardino de la Serna
Rutherford Appleton Laboratory, United Kingdom Research and Innovation, United Kingdom. - Wen Shi
Translational Imaging Center, University of Southern California, CA. - Ashleigh Solano
Biochemistry and Molecular Biology, University of Melbourne, Australia. - Peiyu Wang
Biomedical Engineering, University of Southern California, CA. - Zhongying Wang
The Bridge Institute, University of Southern California, CA. - Joyce Jia Wen Wong
Structural Biology, Stanford University, CA.