Laboratory for Fluorescence Dynamics

A NIH research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Flyer

LFD Workshop Flyer

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

Carl Zeiss Inc. Logo

Olympus Logo

Leica Microsystems

ISS Inc.

PCO AG

Globals Software

University of California, Irvine

Calendars

Notes

  • Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
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Abbreviations

  • DIVER: Deep Imaging Via Enhanced-photon Recovery
  • FCS: Fluorescence Correlation Spectroscopy
  • FLIM: Fluorescence Lifetime Imaging
  • FRET: Förster Resonance Energy Transfer
  • ICS: Image Correlation Spectroscopy
  • iMSD: Image Mean Square Displacement
  • N&B: Number and Brightness
  • pCF: Pair Correlation Function
  • PCH: Photon Counting Histogram
  • PSF: Point Spread Function
  • PT: Particle Tracking
  • RICS: Raster Image Correlation Spectroscopy
  • SPIM: Single Plane Illumination Microscopy
  • STED: Stimulated Emission Depletion
  • more...

13th LFD Workshop (2018)

The "13th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 22–26, 2018, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.

Lecture Recordings

Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.

Agenda and Downloads

Monday, October 22, 2018

Tuesday, October 23, 2018

Wednesday, October 24, 2018

  • Breakfast
  • 09:00-09:45Lecture 8. Enrico Gratton: The Phasor approach to FLIM and FRET PDF
  • 09:45-10:30Lecture 9. Michelle Digman: FLIM PDF
  • Break
  • 11:00-11:45Lecture 10. Leonel Malacrida: Spectral phasors PDF
  • 11:45-12:15Application talk 5. Rachel Cinco-Hedde: Applications of FLIM.
  • 12:15-12:35Application talk 6. Ning Ma: Embryo development by FLIM.
  • Lunch
  • 13:30-14:00Application talk 7. Hongtao Chen: FLIM with cameras.
  • 14:00-17:30Laboratory training.
  • 18:30-20:00Banquet at Avila's El Ranchito, Newport Beach.

Thursday, October 25, 2018

  • Breakfast
  • 09:00-09:45Lecture 11. Hongtao Chen: Advanced instrumentation PDF
  • 09:45-10:30Lecture 12. Alexander Vallmitjana Lees: Particle tracking PDF
  • Break
  • 11:00-11:45Application talk 8. Alexander Dvornikov: Deep tissue imaging (DIVER).
  • 11:45-12:15Application talk 9. Suman Ranjit: Applications of DIVER.
  • 12:15-12:35Application talk 10. Enrico Gratton: The iMSD approach to diffusion analysis.
  • Lunch
  • 13:30-14:00Application talk 11. Enrico Gratton: Orbital tracking in cells.
  • 14:00-17:30Laboratory training.
  • 17:30-17:45Sponsor talk 3. Beniamino Barbieri (ISS Inc): A short presentation of our products and their application.
  • 17:45-18:30Application talk 12. Lorenzo Scipioni: Applications of the Airy detector.
  • Dinner

Friday, October 26, 2018

  • Breakfast
  • 09:00-12:15Laboratory training.
  • 12:15-12:35Symposium.
  • Lunch
  • 13:30-15:30Group presentations.
  • 15:30-16:00Conclusion.

Lecturers

  1. Beniamino Barbieri
    ISS (Instrumenzione Scientificia Sperimentale) Inc., Champaign, IL
  2. Hongtao Chen
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  3. Rachel Cinco-Hedde
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  4. Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  5. Alexander Dvornikov
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  6. Enrico Gratton
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  7. Per Niklas Hedde
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  8. David M Jameson
    Department of Cell and Molecular Biology, University of Hawaii at Manoa
  9. Alexander Vallmitjana Lees
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  10. Ning Ma
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  11. Leonel Malacrida
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  12. Scott Metzler
    PCO-Tech, WA
  13. Giulia Ossato
    Leica Microsystems Inc., Germany
  14. Suman Ranjit
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  15. Lorenzo Scipioni
    Laboratory for Fluorescence Dynamics, University of California, Irvine

Registrants

  1. Rayna Addabbo
    Chemistry, University of Wisconsin-Madison, WI.
  2. Basello Davide Alessandro
    Laboratory of RNA Biology, Institute of Molecular Genetics, Czech Republic.
  3. Tala Allbutt
    ACMM, University of Sydney, Australia.
  4. Josue A Benjamin-Rivera
    Chemistry, University of Puerto Rico - Rio Piedras, Puerto Rico.
  5. Timothy Bilash
    La Jolla, CA.
  6. Chris Bjornsson
    Carl Zeiss Microscopy LLC, NY.
  7. Shanshan Cai
    Biomedical Engineering, University of Southern California, CA.
  8. Hsiao Ju Chaing
    Translational Imaging Center, University of Southern California, CA.
  9. Elizabeth Hinde
    University of Melbourne, Australia.
  10. Vidanage Nuwan Charitha Karunaratne
    Costa Mesa, CA.
  11. Sharon King
    Light Microscopy Center, St Jude Children's Research Hospital, TN.
  12. Nikita Kumari
    School of Molecular Sciences, Arizona State University, AZ.
  13. Adán Lara
    Cellular and Molecular Neurobiology, Universidad Nacional Autónoma de México, México.
  14. Kaitao Li
    Biomedical Engineering, Georgia Institute of Technology, GA.
  15. Maximilian Matthies
    Cardiovascular and Renal Research, University of Southern Denmark, Institute for Molecular Medicine, Denmark.
  16. Mehrnaz Mehrabi
    Biomedical Engineering, University of California, Irvine, CA.
  17. Fatemeh Jelvhei Moghaddam
    Pharma, MultiOmyx, NeoGenomics Laboratories, CA.
  18. Abira Rajah
    Physiology, McGill University, Canada.
  19. Saba Ranjbar
    Chemical Engineering and Materials Science, University of California, Irvine, CA.
  20. Yevgeniy Romin
    Molecular Cytology Core Facility, Memorial Sloan Kettering Cancer Center, NY.
  21. Jorge Bernardino de la Serna
    Rutherford Appleton Laboratory, United Kingdom Research and Innovation, United Kingdom.
  22. Wen Shi
    Translational Imaging Center, University of Southern California, CA.
  23. Ashleigh Solano
    Biochemistry and Molecular Biology, University of Melbourne, Australia.
  24. Peiyu Wang
    Biomedical Engineering, University of Southern California, CA.
  25. Zhongying Wang
    The Bridge Institute, University of Southern California, CA.
  26. Joyce Jia Wen Wong
    Structural Biology, Stanford University, CA.