Flyer
Sponsors
We gratefully acknowledge the sponsorship of the following organizations:
Calendars
Abbreviations
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- MPM: Multiphoton microscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- PT: Particle Tracking
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- STED: Stimulated Emission Depletion
- more...
14th LFD Workshop (2019)
The "14th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 21–25, 2019, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 21, 2019
- Breakfast
- 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Lorenzo Scipioni: Scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Introduction to Phasors
- 12:15-12:35 – Posters.
- Lunch
- 13:30-14:00 – Application talk 2. Francesco Palomba: Applications of FCS.
- 14:00-17:30 – Computer training on FCS. Download computing tutorials and data files .
- Dinner
Tuesday, October 22, 2019
- Breakfast
- 09:00-09:45 – Lecture 4. Per Niklas Hedde: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Lorenzo Scipioni: Number and brightness analysis (N&B)
- Break
- 11:00-11:45 – Lecture 6. Enrico Gratton: The pair correlation approach (pCF)
- 11:45-12:15 – Lecture 7. Michelle Digman: Cross-RICS and cross-N&B
- 12:15-12:35 – Application talk 3. Per Niklas Hedde: Single plane illumination microscopy (SPIM).
- Lunch
- 13:30-14:00 – Application talk 4. Michelle Digman: Applications of N&B.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence
- Dinner
Wednesday, October 23, 2019
- Breakfast
- 09:00-09:45 – Lecture 8. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 9. Michelle Digman: FLIM
- Break
- 11:00-11:45 – Lecture 10. Mihaela Balu: Multiphoton microscopy (MPM) of skin
- 11:45-12:15 – Application talk 5. Rachel Cinco-Hedde: Applications of FLIM.
- 12:15-12:35 – Application talk 6. Michelle Digman: Embryo development by FLIM.
- Lunch
- 13:30-14:00 – Application talk 7. Enrico Gratton: FLIM with cameras.
- 14:00-17:30 – Laboratory training.
- 18:30-20:00 – Banquet at Harborside Restaurant, Newport Beach.
Thursday, October 24, 2019
- Breakfast
- 09:00-09:45 – Lecture 11. Hongtao Chen: Advanced instrumentation
- 09:45-10:30 – Lecture 12. Alexander Vallmitjana Lees: Particle tracking
- Break
- 11:00-11:45 – Application talk 8. Alexander Dvornikov: Deep tissue imaging (DIVER).
- 11:45-12:15 – Application talk 9. Simon Leemans: Adaptive Optics for DIVER.
- 12:15-12:35 – Application talk 10. Tam Vu: Gene expression by FLIM.
- Lunch
- 13:30-14:00 – Application talk 11. Mihaela Balu: Multiphoton microscopy of skin.
- 14:00-17:30 – Laboratory training.
- 17:30-18:30 – Application talk 12. Lorenzo Scipioni: Airy detector.
- Dinner
Friday, October 25, 2019
- Breakfast
- 09:00-12:15 – Laboratory training.
- 12:15-12:35 – Symposium.
- Lunch
- 13:30-15:30 – Symposium presentations.
- 15:30-16:00 – Conclusion.
Lecturers
- Mihaela Balu
Beckman Laser Institute, University of California, Irvine - Hongtao Chen
Laboratory for Fluorescence Dynamics, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Simon Leemans
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Vallmitjana Lees
Laboratory for Fluorescence Dynamics, University of California, Irvine - Francesco Palomba
Laboratory for Fluorescence Dynamics, University of California, Irvine - Lorenzo Scipioni
Laboratory for Fluorescence Dynamics, University of California, Irvine - Tam Vu
Biomedical Engineering, University of California, Irvine
Registrants
- Julio A. Ayala Angulo
Molecular Biology & Biochemistry, University of California, Irvine, CA. - Johanna Buschhaus
Radiology/Biomedical Engineering, University of Michigan, MI. - Kirk James Czymmek
Integrated Microscopy Facility, Donald Danforth Plant Science Center, MO. - Tara Essock-Burns
Kewalo Marine Laboratory, University of Hawaii at Manoa, HI. - Mylene Ferrolino
Structural Biology, St. Jude Children's Research Hospital, TN. - Julianna Goelzer
Biomolecular Science, Boise State University, ID. - Agnes Janoshazi
National Institute of Environmental Health Sciences (NIEHS), National Institutes of Health, NC. - Sean Kuan
Developmental and Cell Biology, University of California, Irvine, CA. - Alan Ly
Physiology and Biophysics, University of California, Irvine, CA. - Tim Mann
Ingham Institute, Western Sydney University, Australia. - Zachary Oberholtzer
Protein Biochemistry, Regeneron Pharmaceuticals, NY. - Jennifer L. Peters
CISR, St. Jude Children's Research Hospital, TN. - Wim Pomp
Gene Regulation, Netherlands Cancer Institute, The Netherlands. - Sunil Shah
ISS Inc., IL. - Rinzhin Tshering Sherpa
Pharmacology, University of Nevada Reno, NV. - Dilip Shrestha
Weatherall Institute of Molecular Medicine, University of Oxford, United Kingdom. - Vinod Kumar Subramani
Molecular Cell Biology, Sungkyunkwan University, Republic of Korea. - Elena Thomas
Chemistry, University of Washington, WA. - Michele Tolbert
Structural Biology, St. Jude Children's Research Hospital, TN. - Leoncio Alejandro Vergara
Center for Translational Cancer Research (CTCR), Institute of Biosciences and Technology-Texas A&M University, TX.