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Calendars
Abbreviations
- AI: Artificial Intelligence
- DIVER: Deep Imaging Via Enhanced-photon Recovery
- FCS: Fluorescence Correlation Spectroscopy
- FFS: Fluorescence Fluctuation Spectroscopy
- FLIM: Fluorescence Lifetime Imaging
- FRET: Förster Resonance Energy Transfer
- ICS: Image Correlation Spectroscopy
- iMSD: Image Mean Square Displacement
- MPM: Multiphoton microscopy
- N&B: Number and Brightness
- pCF: Pair Correlation Function
- PCH: Photon Counting Histogram
- PSF: Point Spread Function
- RICS: Raster Image Correlation Spectroscopy
- SPIM: Single Plane Illumination Microscopy
- more...
15th LFD Workshop (2021)
The "15th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 25–29, 2021, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.
Due to the COVID-19 pandemic, lectures, application talks, symposium, and computer-based training were held virtually via Zoom. The hands-on laboratory training at the LFD facility were limited to UCI members (faculty, staff, students, postdocs) and corporate registrants.
Lecture Recordings
Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.
Agenda and Downloads
Monday, October 25, 2021
- 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
- 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
- Break
- 11:00-11:45 – Lecture 3. Lorenzo Scipioni: Scanning FCS (sFCS)
- 11:45-12:15 – Application talk 1. David Jameson: Fluorescence polarization
- 12:15-12:35 – Application talk 2. Francesco Palomba: Application of FCS.
- Break
- 13:30-17:30 – Computer training on FCS. Download computing tutorials , software and data files .
Tuesday, October 26, 2021
- 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
- 09:45-10:30 – Lecture 5. Per Niklas Hedde: Number and brightness analysis (N&B)
- Break
- 11:00-11:45 – Lecture 6. Francesco Palomba: The pair correlation approach (pCF)
- 11:45-12:15 – Application talk 3. Enrico Gratton: The iMSD approach to diffusion analysis.
- 12:15-12:35 – Application talk 4. Per Niklas Hedde: Single plane illumination microscopy (SPIM).
- Break
- 13:30-14:00 – Application talk 5. Giulia Tedeschi: Application of FFS to membrane proteins.
- 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials , software and data files .
- 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence.
Wednesday, October 27, 2021
- 09:00-09:45 – Lecture 7. Enrico Gratton: The Phasor approach to FLIM and FRET
- 09:45-10:30 – Lecture 8. Michelle Digman: FLIM biosensors and metabolic index.
- Break
- 11:00-11:45 – Lecture 9. Leonel Malacrida: Spectral phasors
- 11:45-12:15 – Application talk 6. Lorenzo Scipioni: Spectral FLIM.
- 12:15-12:35 – Application talk 7. Leonel Malacrida: Laurdan spectral phasors.
- Break
- 13:30-14:00 – Application talk 8. Suman Ranjit: Lifetime analysis of tissue samples.
- 14:00-17:30 – Laboratory training at LFD facility.
Thursday, October 28, 2021
- 09:00-09:45 – Lecture 10. Alexander Vallmitjana Lees: Clustering and unmixing in the phasor space
- 09:45-10:30 – Application talk 9. Austin Lefebvre: Software mitometer.
- Break
- 11:00-11:45 – Lecture 11. Enrico Gratton: AI for the analysis of phasors.
- 11:45-12:05 – Application talk 10. Alexander Dvornikov: Deep tissue imaging (DIVER)
- 12:05-12:35 – Lecture 12. Mihaela Balu: Instrumentation for skin studies.
- Break
- 13:30-14:00 – Application talk 11. Belén Torrado: Linear filter.
- 14:00-17:30 – Laboratory training at LFD facility.
- 17:30-18:00 – Application talk 12. Mihaela Balu: Skin studies.
Friday, October 29, 2021
- 09:00-12:15 – Laboratory training at LFD facility.
- 12:35-15:30 – Student Symposium.
- 15:30-16:00 – Conclusion.
Lecturers
- Mihaela Balu
Beckman Laser Institute, University of California, Irvine - Michelle A Digman
Laboratory for Fluorescence Dynamics, University of California, Irvine - Alexander Dvornikov
Laboratory for Fluorescence Dynamics, University of California, Irvine - Enrico Gratton
Laboratory for Fluorescence Dynamics, University of California, Irvine - Per Niklas Hedde
Laboratory for Fluorescence Dynamics, University of California, Irvine - David M Jameson
Department of Cell and Molecular Biology, University of Hawaii at Manoa - Leonel Malacrida
Institut Pasteur de Montevideo-Universidad de la República del Uruguay - Suman Ranjit
Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, D.C. - Alexander Vallmitjana Lees
Biomedical Engineering, University of California, Irvine - Austin Lefebvre
Laboratory for Fluorescence Dynamics, University of California, Irvine - Francesco Palomba
Laboratory for Fluorescence Dynamics, University of California, Irvine - Lorenzo Scipioni
Laboratory for Fluorescence Dynamics, University of California, Irvine - Giulia Tedeschi
Laboratory for Fluorescence Dynamics, University of California, Irvine - Belén Torrado
Laboratory for Fluorescence Dynamics, University of California, Irvine
Registrants
- Ashwin Balakrishnan
Rudolf Virchow Center, Molecular Microscopy, University of Würzburg, Germany. - Francesco Giovanni Barone
Molecular Physiology and Cell Signalling, University of Liverpool, United Kingdom. - Sungjun Beck
School of Medicine, University of California, Irvine, CA. - Jose Bermudez
Mathematical, Computational and Systems Biology, University of California, Irvine, CA. - William Bodeen
Developmental Biology, St. Jude, TN. - Peng Chen
Bioengineering, Clemson University, SC. - Susobhan Choudhury
Molecular Microscopy, University of Würzburg, Germany. - Sean Cleary
Cell and Molecular Physiology, Loyola University Chicago, IL. - Robert Cohen
Biochemistry and Molecular Biology, Colorado State University, CO. - Ulas Coskun
Microscopy, ISS Inc., IL. - Ozlem Dilek
Chemistry, University of the District of Columbia, DC. - Amanda Durkin
Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA. - Janusz Franco-Barraza
Cancer Signaling and Epigenetics Program, Fox Chase Cancer Center, PA. - Mike Friedrich
Rudolf Virchow Center, University of Würzburg, Germany. - Rajen Kumar Goutam
Physics and Astronomy, Clemson University, SC. - F. Javier Díez Guerra
Molecular Biology, Universidad Autonoma de Madrid, Spain. - Daniele Gulli
Physics and Chemistry, Universuty of Palermo, Italy. - George Hamilton
Physics and Astronomy, Clemson University, SC. - Alan K. Hauser
Arvetas Biosciences, CA. - Alex Hsu
Biological Chemistry, University of California, Irvine, CA. - Nan Hultgren
Ophthalmology, University of California, Los Angeles, CA. - Peter Johnson
Oncology, Georgetown University Medical Center, DC. - Chelsea Jones
Chemistry, University of California, Irvine, CA. - Satya Karri
School of Medicine, Biological Chemistry, University of California, Irvine, CA. - Adil Khan
Bioengineering, University of Texas at Dallas, TX. - Ajeet Kumar
Biological Sciences, Korea Advanced Institute of Science and Technology, South Korea. - Gretchen Kunz
Biomolecular Sciences, Boise State University, ID. - Danielle Latham
Physics and Astronomy, Clemson University, SC. - Francesco Marangoni
Physiology, University of California, Irvine, CA. - Michael Nelson
Biomedical Engineering, University of Wisconsin - Madison, WI. - Shivashankar Othy
Physiology and Biophysics, University of California, Irvine, CA. - Carolina dos Santos Passos
Biochemistry and Molecular Biology, Colorado State University, CO. - Ricardo Santana
Biomedical Engineering, University of California, Irvine, CA. - Kristina Shrestha
Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA. - Janet Elise Sorrells
Bioengineering, University of Illinois at Urbana-Champaign, IL. - Juvinch Vicente
Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA. - Bijing Xiong
Environmental Microbiology, Helmholtz Center for Environmental Research, Germany. - Tingting Yao
Biochemistry and Molecular Biology, Colorado State University, CO. - Qingrong Zhang
Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Belgium.