Laboratory for Fluorescence Dynamics

A national research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Flyer

LFD Workshop Flyer

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

Globals Software

University of California, Irvine

Calendars

Abbreviations

  • AI: Artificial Intelligence
  • DIVER: Deep Imaging Via Enhanced-photon Recovery
  • FCS: Fluorescence Correlation Spectroscopy
  • FFS: Fluorescence Fluctuation Spectroscopy
  • FLIM: Fluorescence Lifetime Imaging
  • FRET: Förster Resonance Energy Transfer
  • ICS: Image Correlation Spectroscopy
  • iMSD: Image Mean Square Displacement
  • MPM: Multiphoton microscopy
  • N&B: Number and Brightness
  • pCF: Pair Correlation Function
  • PCH: Photon Counting Histogram
  • PSF: Point Spread Function
  • RICS: Raster Image Correlation Spectroscopy
  • SPIM: Single Plane Illumination Microscopy
  • more...

15th LFD Workshop (2021)

The "15th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 25–29, 2021, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.

Due to the COVID-19 pandemic, lectures, application talks, symposium, and computer-based training were held virtually via Zoom. The hands-on laboratory training at the LFD facility were limited to UCI members (faculty, staff, students, postdocs) and corporate registrants.

Lecture Recordings

Lecture notes and recordings are for personal use only and may not be redistributed without the speaker's permission.

Agenda and Downloads

Monday, October 25, 2021

Tuesday, October 26, 2021

Wednesday, October 27, 2021

  • 09:00-09:45Lecture 7. Enrico Gratton: The Phasor approach to FLIM and FRET PDF
  • 09:45-10:30Lecture 8. Michelle Digman: FLIM biosensors and metabolic index.
  • Break
  • 11:00-11:45Lecture 9. Leonel Malacrida: Spectral phasors PDF
  • 11:45-12:15Application talk 6. Lorenzo Scipioni: Spectral FLIM.
  • 12:15-12:35Application talk 7. Leonel Malacrida: Laurdan spectral phasors.
  • Break
  • 13:30-14:00Application talk 8. Suman Ranjit: Lifetime analysis of tissue samples.
  • 14:00-17:30Laboratory training at LFD facility.

Thursday, October 28, 2021

  • 09:00-09:45Lecture 10. Alexander Vallmitjana Lees: Clustering and unmixing in the phasor space PDF
  • 09:45-10:30Application talk 9. Austin Lefebvre: Software mitometer.
  • Break
  • 11:00-11:45Lecture 11. Enrico Gratton: AI for the analysis of phasors.
  • 11:45-12:05Application talk 10. Alexander Dvornikov: Deep tissue imaging (DIVER) PDF
  • 12:05-12:35Lecture 12. Mihaela Balu: Instrumentation for skin studies.
  • Break
  • 13:30-14:00Application talk 11. Belén Torrado: Linear filter.
  • 14:00-17:30Laboratory training at LFD facility.
  • 17:30-18:00Application talk 12. Mihaela Balu: Skin studies.

Friday, October 29, 2021

  • 09:00-12:15Laboratory training at LFD facility.
  • 12:35-15:30Student Symposium.
  • 15:30-16:00Conclusion.

Lecturers

  1. Mihaela Balu
    Beckman Laser Institute, University of California, Irvine
  2. Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  3. Alexander Dvornikov
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  4. Enrico Gratton
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  5. Per Niklas Hedde
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  6. David M Jameson
    Department of Cell and Molecular Biology, University of Hawaii at Manoa
  7. Leonel Malacrida
    Institut Pasteur de Montevideo-Universidad de la República del Uruguay
  8. Suman Ranjit
    Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, D.C.
  9. Alexander Vallmitjana Lees
    Biomedical Engineering, University of California, Irvine
  10. Austin Lefebvre
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  11. Francesco Palomba
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  12. Lorenzo Scipioni
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  13. Giulia Tedeschi
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  14. Belén Torrado
    Laboratory for Fluorescence Dynamics, University of California, Irvine

Registrants

  1. Ashwin Balakrishnan
    Rudolf Virchow Center, Molecular Microscopy, University of Würzburg, Germany.
  2. Francesco Giovanni Barone
    Molecular Physiology and Cell Signalling, University of Liverpool, United Kingdom.
  3. Sungjun Beck
    School of Medicine, University of California, Irvine, CA.
  4. Jose Bermudez
    Mathematical, Computational and Systems Biology, University of California, Irvine, CA.
  5. William Bodeen
    Developmental Biology, St. Jude, TN.
  6. Peng Chen
    Bioengineering, Clemson University, SC.
  7. Susobhan Choudhury
    Molecular Microscopy, University of Würzburg, Germany.
  8. Sean Cleary
    Cell and Molecular Physiology, Loyola University Chicago, IL.
  9. Robert Cohen
    Biochemistry and Molecular Biology, Colorado State University, CO.
  10. Ulas Coskun
    Microscopy, ISS Inc., IL.
  11. Ozlem Dilek
    Chemistry, University of the District of Columbia, DC.
  12. Amanda Durkin
    Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA.
  13. Janusz Franco-Barraza
    Cancer Signaling and Epigenetics Program, Fox Chase Cancer Center, PA.
  14. Mike Friedrich
    Rudolf Virchow Center, University of Würzburg, Germany.
  15. Rajen Kumar Goutam
    Physics and Astronomy, Clemson University, SC.
  16. F. Javier Díez Guerra
    Molecular Biology, Universidad Autonoma de Madrid, Spain.
  17. Daniele Gulli
    Physics and Chemistry, Universuty of Palermo, Italy.
  18. George Hamilton
    Physics and Astronomy, Clemson University, SC.
  19. Alan K. Hauser
    Arvetas Biosciences, CA.
  20. Alex Hsu
    Biological Chemistry, University of California, Irvine, CA.
  21. Nan Hultgren
    Ophthalmology, University of California, Los Angeles, CA.
  22. Peter Johnson
    Oncology, Georgetown University Medical Center, DC.
  23. Chelsea Jones
    Chemistry, University of California, Irvine, CA.
  24. Satya Karri
    School of Medicine, Biological Chemistry, University of California, Irvine, CA.
  25. Adil Khan
    Bioengineering, University of Texas at Dallas, TX.
  26. Ajeet Kumar
    Biological Sciences, Korea Advanced Institute of Science and Technology, South Korea.
  27. Gretchen Kunz
    Biomolecular Sciences, Boise State University, ID.
  28. Danielle Latham
    Physics and Astronomy, Clemson University, SC.
  29. Francesco Marangoni
    Physiology, University of California, Irvine, CA.
  30. Michael Nelson
    Biomedical Engineering, University of Wisconsin - Madison, WI.
  31. Shivashankar Othy
    Physiology and Biophysics, University of California, Irvine, CA.
  32. Carolina dos Santos Passos
    Biochemistry and Molecular Biology, Colorado State University, CO.
  33. Ricardo Santana
    Biomedical Engineering, University of California, Irvine, CA.
  34. Kristina Shrestha
    Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA.
  35. Janet Elise Sorrells
    Bioengineering, University of Illinois at Urbana-Champaign, IL.
  36. Juvinch Vicente
    Beckman Laser Institute and Medical Clinic, University of California, Irvine, CA.
  37. Bijing Xiong
    Environmental Microbiology, Helmholtz Center for Environmental Research, Germany.
  38. Tingting Yao
    Biochemistry and Molecular Biology, Colorado State University, CO.
  39. Qingrong Zhang
    Louvain Institute of Biomolecular Science and Technology, Université Catholique de Louvain, Belgium.