Laboratory for Fluorescence Dynamics

Laboratory for Fluorescence Dynamics
A national research center for biomedical fluorescence spectroscopy at the University of California, Irvine

Site Menu

LFD Workshop

Flyer

LFD Workshop Flyer

Sponsors

We gratefully acknowledge the sponsorship of the following organizations:

ISS Inc.

Spectra-Physics.

Globals Software

University of California, Irvine

Allen Institute

Calendars

Abbreviations

  • AI: Artificial Intelligence
  • DIVER: Deep Imaging Via Enhanced-photon Recovery
  • FCS: Fluorescence Correlation Spectroscopy
  • FFS: Fluorescence Fluctuation Spectroscopy
  • FLIM: Fluorescence Lifetime Imaging
  • FRET: Förster Resonance Energy Transfer
  • ICS: Image Correlation Spectroscopy
  • iMSD: Image Mean Square Displacement
  • MPM: Multiphoton microscopy
  • N&B: Number and Brightness
  • pCF: Pair Correlation Function
  • PCH: Photon Counting Histogram
  • PSF: Point Spread Function
  • RICS: Raster Image Correlation Spectroscopy
  • SPIM: Single Plane Illumination Microscopy
  • more...

16th LFD Workshop (2022)

The "16th LFD Workshop in Advanced Fluorescence Imaging and Dynamics" was held on October 24–28, 2022, at the Laboratory for Fluorescence Dynamics (LFD), University of California, Irvine.

Agenda and Downloads

Monday, October 24, 2022

  • 09:00-09:45 – Lecture 1. Enrico Gratton: Fluorescence correlation spectroscopy (FCS)
  • 09:45-10:30 – Lecture 2. Enrico Gratton: The photon counting histogram (PCH)
  • Break
  • 11:00-11:45 – Lecture 3. Lorenzo Scipioni: Scanning FCS (sFCS)
  • 11:45-12:15 – Application talk 1.Francesco Palomba: Application of FCS.
  • 12:15-12:35 – Application talk 2. David Jameson: Time-Resolved Fluorescence and Phasors
  • Break
  • 13:30-17:30 – Computer training on FCS. Download computing tutorials, software and data files.

Tuesday, October 25, 2022

  • 09:00-09:45 – Lecture 4. Michelle Digman: Raster image correlation spectroscopy (RICS)
  • 09:45-10:30 – Lecture 5. Michelle Digman: Number and brightness analysis (N&B)
  • Break
  • 11:00-11:45 – Lecture 6. Francesco Palomba: The pair correlation approach (pCF)
  • 11:45-12:15 – Application talk 3. Enrico Gratton: The iMSD approach to diffusion analysis.
  • 12:15-12:35 – Application talk 4. Yu-Kai Haung: Single plane illumination microscopy (SPIM).
  • Break
  • 13:30-14:00 – Application talk 5. Giulia Tedeschi: Application of FFS to membrane proteins.
  • 14:00-17:30 – Computer training on RICS and FLIM. Download computing tutorials, software and data files.
  • 17:30-18:30 – Special lecture 1. David Jameson: A nano-history of fluorescence.

Wednesday, October 26, 2022

  • 09:00-09:45 – Lecture 7. Enrico Gratton: The Phasor approach to FLIM and FRET
  • 09:45-10:30 – Lecture 8. Leonel Malacrida: Spectral phasors
  • Break
  • 11:00-11:30 – Application talk 6. Lorenzo Scipioni: Spectral FLIM.
  • 11:30-12:15 – Lecture 9. Jennifer Prescher: Bioluminescence
  • 12:15-12:35 – Application talk 7. Lila Halbers and Mariana Navarro: Bioluminescence and Spectral phasors.
  • Break
  • 13:30-14:00 – Application talk 8. Suman Ranjit: Lifetime analysis of tissue samples.
  • 14:00-17:30 – Laboratory training at LFD facility.

Thursday, October 27, 2022

  • 09:00-09:45 – Lecture 10. Alexander Vallmitjana: Clustering and unmixing in the phasor space
  • 09:45-11:00 – Discussion Panel. Michelle Digman and Emi Embler: Addressing Equity and Inclusion.
  • 11:00-11:45 – Lecture 11. Enrico Gratton: AI for the analysis of phasors.
  • 11:45-12:05 – Application talk 9. Belen Torrado: Deep tissue imaging (DIVER)
  • 12:05-12:35 – Lecture 12. Anand Ganesan: Instrumentation for skin studies.
  • Break
  • 13:30-14:00 – Application talk 10. Belén Torrado: Sine/Cosine filter for spectral phasor analysis.
  • 14:00-17:30 – Laboratory training at LFD facility.

Friday, October 28, 2022

  • 09:00-12:15 – Laboratory training at LFD facility.
  • 13:30-14:00 – Student Symposium.
  • 14:00-15:30 – Conclusion.

Lecturers

  1. Michelle A Digman
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  2. Anand Ganesan
    Beckman Laser Institute, University of California, Irvine
  3. Enrico Gratton
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  4. Lila Halbers
    The Prescher Lab, University of California, Irvine
  5. David M Jameson
    Department of Cell and Molecular Biology, University of Hawaii at Manoa
  6. Leonel Malacrida
    Institut Pasteur de Montevideo-Universidad de la República del Uruguay
  7. Mariana Navarro
    The Prescher Lab, University of California, Irvine
  8. Suman Ranjit
    Biochemistry and Molecular and Cellular Biology, Georgetown University, Washington, D.C.
  9. Francesco Palomba
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  10. Jennifer Prescher
    The Prescher Lab, University of California, Irvine
  11. Lorenzo Scipioni
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  12. Giulia Tedeschi
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  13. Belén Torrado
    Laboratory for Fluorescence Dynamics, University of California, Irvine
  14. Alexander Vallmitjana
    Biomedical Engineering, University of California, Irvine

Registrants

Laboratory for Fluorescence Dynamics.  University of California, Irvine.  Department of Biomedical Engineering.  3208 Natural Sciences II.  Irvine, CA 92697‑2715, USA.  Phone: +1‑949‑824‑7085.  Email: lfd@uci.edu.  Copyright © 2006‑2024.